Construct: ORF TRCN0000492310
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021580.2_s317c1
- DNA Barcode:
- ATGCGCAGGGATAAGCAAAATGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RSPH3 (83861)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492310
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83861 | RSPH3 | radial spoke head 3 | NM_031924.6 | 100% | 100% | |
| 2 | human | 83861 | RSPH3 | radial spoke head 3 | NM_001346418.1 | 82.8% | 82.8% | 629_630ins288 |
| 3 | human | 83861 | RSPH3 | radial spoke head 3 | XR_001743668.2 | 72.6% | 1_450del;2131_2314del | |
| 4 | human | 83861 | RSPH3 | radial spoke head 3 | XM_017011347.2 | 51.4% | 51.4% | 0_1ins816 |
| 5 | human | 83861 | RSPH3 | radial spoke head 3 | XM_024446566.1 | 51.4% | 51.4% | 0_1ins816 |
| 6 | human | 83861 | RSPH3 | radial spoke head 3 | XR_001743669.2 | 51% | 1_450del;2131_3293del | |
| 7 | human | 83861 | RSPH3 | radial spoke head 3 | NR_144434.1 | 26.9% | 1_211del;1892_6237del | |
| 8 | human | 83861 | RSPH3 | radial spoke head 3 | XR_001743671.2 | 22.2% | 0_1ins202;427_428ins142;1337_5672del | |
| 9 | human | 83861 | RSPH3 | radial spoke head 3 | XR_001743670.2 | 21.5% | 1_450del;1079_1080ins288;1843_6178del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1749
- ORF length:
- 1680
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gacggtcaag ccagccaagg ccgcctccct agccaggaac ctcgcaaagc 121 gcaggcgcac ctacctgggt ggcgcggctg ggcggagtca agagccggaa gtcccttgtg 181 ccgcagtcct tcccgggaaa cctggggacc ggaactgccc ggagttcccg cccccagaca 241 gaaccctggg ttgctgggca accgatgcgg cccccgcagc tggtctctgt ggtgctggga 301 gcgagcccag tatagctccc acctcctgcg ctggtaacct cccgtcccgc ccgcctccct 361 tgttgtcgcc tctcctcgcg tcgcggaatc cttgcccttg gcactactta catctctccg 421 ggtcccacaa caccttagcg cccacctgct tcaaagccaa gctccaccga aagcgaggca 481 gtcagccccc ggacatggcc tcagcgctga ctgatcgcac ctctcgggcc ccgagcacct 541 acacctacac cagccggccc cgagcactgc cctgccagcg cagccgttac cgggacagcc 601 tgacgcagcc agatgaagaa cctatgcatt atggaaacat aatgtatgac agaagggtaa 661 ttcgaggtaa cacttatgca ctccagacag ggccactgct cggacggcct gattctctag 721 agctccagag acaacgggag gctaggaaga gggctcttgc cagaaaacaa gcccaagagc 781 agctcagacc acaaacacct gaacctgtgg aaggcagaaa gcatgtcgat gtgcaaacag 841 aattatacct tgaagaaatt gctgatcgca taatagaagt tgatatggaa tgccaaacag 901 atgcattttt ggacagacca ccaacaccac tctttattcc tgccaaaact ggcaaagatg 961 tggccaccca aatactagaa ggagagctct ttgactttga tcttgaagtt aaaccagtgt 1021 tagaagtttt ggtggggaag acaattgagc agtctcttct ggaagtaatg gaagaagaag 1081 agctggctaa cctgcgggcc agtcagcgtg agtatgaaga actacggaat agtgaacgtg 1141 ctgaagttca acgacttgaa gagcaagaga ggcgacaccg agaagaaaaa gaacggcgta 1201 agaaacagca gtgggaaata atgcacaagc acaacgagac atcacaaaaa atcgccgccc 1261 gagcatttgc acagcgttac ctggctgacc ttctcccgtc tgtttttggc agcctcaggg 1321 atagtggcta cttttatgat cccattgaaa gagatattga gataggattt cttccatggc 1381 taatgaatga agttGAAAAA ACCATGGAAT ATAGCATGGT GGGAAGAACA GTGCTTGACA 1441 TGTTGATCCG TGAGGTGGTT GAAAAGAGGC TGTGTATGTA TGAGCATGGG GAAGACACAC 1501 ATCAGTCTCC AGAACCCGAG GATGAGCCTG GTGGTCCTGG AGCAATGACA GAGTCACTGG 1561 AGGCCTCTGA ATTCCTGGAG CAGAGCATGT CACAGACACG GGAGCTGCTT TTAGATGGAG 1621 GCTACCTACA AAGAACAACA TATGACAGAA GGTCATCCCA GGAAAGGAAG TTTATGGAAG 1681 AGAGAGAACT CTTAGGGCAA GATGAAGAAA CAGCAATGAG GAAGTCCTTA GGGGAGGAAG 1741 AATTGTCATA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1801 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1861 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATG CGCAGGGATA AGCAAAATGT 1921 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t