Construct: ORF TRCN0000492312
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005241.2_s317c1
- Derived from:
- ccsbBroadEn_01083
- DNA Barcode:
- GCTGTTCGGTAGTCACGTTCATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NELL2 (4753)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492312
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4753 | NELL2 | neural EGFL like 2 | NM_001145108.1 | 100% | 100% | |
| 2 | human | 4753 | NELL2 | neural EGFL like 2 | NM_006159.2 | 100% | 100% | |
| 3 | human | 4753 | NELL2 | neural EGFL like 2 | XM_005268905.3 | 100% | 100% | |
| 4 | human | 4753 | NELL2 | neural EGFL like 2 | XM_011538396.1 | 100% | 100% | |
| 5 | human | 4753 | NELL2 | neural EGFL like 2 | XM_017019342.2 | 99.3% | 99.2% | 680_694del |
| 6 | human | 4753 | NELL2 | neural EGFL like 2 | XM_017019343.1 | 99.3% | 99.2% | 680_694del |
| 7 | human | 4753 | NELL2 | neural EGFL like 2 | XM_017019344.1 | 99.3% | 99.2% | 680_694del |
| 8 | human | 4753 | NELL2 | neural EGFL like 2 | NM_001145109.1 | 98.6% | 98% | (many diffs) |
| 9 | human | 4753 | NELL2 | neural EGFL like 2 | NM_001145110.1 | 97.2% | 97.2% | 1_69del |
| 10 | human | 4753 | NELL2 | neural EGFL like 2 | NM_001145107.1 | 94.2% | 94.2% | 1_150del |
| 11 | human | 4753 | NELL2 | neural EGFL like 2 | XM_017019341.2 | 93.6% | 93.5% | 1_150del;830_844del |
| 12 | mouse | 54003 | Nell2 | NEL-like 2 | NM_001289653.1 | 86.1% | 93.6% | (many diffs) |
| 13 | mouse | 54003 | Nell2 | NEL-like 2 | XM_006521163.2 | 86.1% | 93.6% | (many diffs) |
| 14 | mouse | 54003 | Nell2 | NEL-like 2 | XM_011245693.1 | 86.1% | 93.6% | (many diffs) |
| 15 | mouse | 54003 | Nell2 | NEL-like 2 | XM_006521164.3 | 84.7% | 91.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2514
- ORF length:
- 2448
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtctcgggtc ttactgagaa cattctgttt gatcttcggt ctcggagcag 121 tttgggggct tggtgtggac ccttccctac agattgacgt cttaacagag ttagaacttg 181 gggagtccac gaccggagtg cgtcaggtcc cggggctgca taatgggacg aaagcctttc 241 tctttcaaga tactcccaga agcataaaag catccactgc tacagctgaa cagttttttc 301 agaagctgag aaataaacat gaatttacta ttttggtgac cctaaaacag acccacttaa 361 attcaggagt tattctctca attcaccact tggatcacag gtacctggaa ctggaaagta 421 gtggccatcg gaatgaagtc agactgcatt accgctcagg cagtcaccgc cctcacacag 481 aagtgtttcc ttacattttg gctgatgaca agtggcacaa gctctcctta gccatcagtg 541 cttcccattt gattttacac attgactgca ataaaattta tgaaagggta gtagaaaagc 601 cctccacaga cttgcctcta ggcacaacat tttggctagg acagagaaat aatgcgcatg 661 gatattttaa gggtataatg caagatgtcc aattacttgt catgccccag ggatttattg 721 ctcagtgccc agatcttaat cgcacctgtc caacttgcaa tgacttccat ggacttgtgc 781 agaaaatcat ggagctacag gatattttag ccaaaacatc agccaagctg tctcgagctg 841 aacagcgaat gaatagattg gatcagtgct attgtgaaag gacttgcacc atgaagggaa 901 ccacctaccg agaatttgag tcctggatag acggctgtaa gaactgcaca tgcctgaatg 961 gaaccatcca gtgtgaaact ctaatctgcc caaatcctga ctgcccactt aagtcggctc 1021 ttgcgtatgt ggatggcaaa tgctgtaagg aatgcaaatc gatatgccaa tttcaaggac 1081 gaacctactt tgaaggagaa agaaatacag tctattcctc ttctggagta tgtgttctct 1141 atgagtgcaa ggaccagacc atgaaacttg ttgagagttc aggctgtcca gctttggatt 1201 gtccagagtc tcatcagata accttgtctc acagctgttg caaagtttgt aaaggttatg 1261 acttttgttc tgaaaggcat aactgcatgg agaattccat ctgcagaaat ctgaatgaca 1321 gggctgtttg tagctgtcga gatggtttta gggctcttcg agaggataat gcctactgtg 1381 aagacatcga tgagtgtgct gaagggcgcc attactgtcg tgaaaataca atgtgtgtca 1441 acaccccggg ttcttttatg tgcatctgca aaactggata catcagaatt gatgattatt 1501 catgtacaga acatgatgag tgtatcacaa atcagcacaa ctgtgatgaa aatgctttat 1561 gcttcaacac tgttggagga cacaactgtg tttgcaagcc gggctataca gggaatggaa 1621 cgacatgcaa agcattttgc aaagatggct gtaggaatgg aggagcctgt attgccgcta 1681 atgtgtgtgc ctgcccacaa ggcttcactg gacccagctg tgaaacggac attgatgaat 1741 gctctgatgg ttttgttcaa tgtgacagtc gtgctaattg cattaacctg cctggatggt 1801 accactgtga gtgcagagat ggctaccatg acaatgggat gttttcacca agtggagaat 1861 cgtgtgaaga tattgatgag tgtgggaccg ggaggcacag ctgtgccaat gataccattt 1921 gcttcaattt ggatggcgga tatgattgtc gatgtcctca tggaaagaat tgcacagggg 1981 actgcatcca tgatggaaaa gttaagcaca atggtcagat ttgggtgttg gaaaatgaca 2041 ggtgctctgt gtgctcatgt cagaatggat tcgttatgtg tcgacggatg gtctgtgact 2101 gtgagaatcc cacagttgat cttttttgct gccctgaatg tgacccaagg cttagtagtc 2161 agtgcctcca tcaaaatggg gaaactttgt ataacagtgg tgacacctgg gtccagaatt 2221 gtcaacagtg ccgctgcttg caaggggaag ttgattgttg gcccctgcct tgcccagatg 2281 tggagtgtga attcagcatt ctcccagaga atgagtgctg cccgcgctgt gtcacagacc 2341 cttgccaggc tgacaccatc cgCAATGACA TCACCAAGAC TTGCCTGGAC GAAATGAATG 2401 TGGTTCGCTT CACCGGGTCC TCTTGGATCA AACATGGCAC TGAGTGTACT CTCTGCCAGT 2461 GCAAGAATGG CCACATCTGT TGCTCAGTGG ATCCACAGTG CCTTCAGGAA CTGTGCCCAA 2521 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2581 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2641 TATCTTGTGG AAAGGACGAG CTGTTCGGTA GTCACGTTCA TAAACGCGTT AAGTCgacaa 2701 tcaacctctg gattacaaaa tttgtgaaag att