Construct: ORF TRCN0000492315
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021127.2_s317c1
- DNA Barcode:
- AATGGCAGCTTGACTTCAATGCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MTNR1A (4543)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492315
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4543 | MTNR1A | melatonin receptor 1A | NM_005958.4 | 100% | 100% | |
| 2 | human | 4543 | MTNR1A | melatonin receptor 1A | XM_011532002.3 | 75.7% | 75.7% | 0_1ins255 |
| 3 | mouse | 17773 | Mtnr1a | melatonin receptor 1A | XM_006509291.2 | 68.3% | 72% | (many diffs) |
| 4 | mouse | 17773 | Mtnr1a | melatonin receptor 1A | XM_011242176.1 | 68% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1122
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcagggc aacggcagcg cgctgcccaa cgcctcccag cccgtgctcc 121 gcggggacgg cgcgcggccc tcgtggctgg cgtccgccct ggcctgcgtc ctcatcttca 181 ccatcgtggt ggacatcctg ggcaacctcc tggtcatcct gtcggtgtat cggaacaaga 241 agctcaggaa cgcaggaaac atctttgtgg tgagcttagc ggtggcagac ctggtggtgg 301 ccatttatcc gtacccgttg gtgctgatgt cgatatttaa caacgggtgg aacctgggct 361 atctgcactg ccaagtcagt gggttcctga tgggcctgag cgtcatcggc tccatattca 421 acatcaccgg catcgccatc aaccgctact gctacatctg ccacagtctc aagtacgaca 481 aactgtacag cagcaagaac tccctctgct acgtgctcct catatggctc ctgacgctgg 541 cggccgtcct gcccaacctc cgtgcaggga ctctccagta cgacccgagg atctactcgt 601 gcaccttcgc ccagtccgtc agctccgcct acaccatcgc cgtggtggtt ttccacttcc 661 tcgtccccat gatcatagtc atcttctgtt acctgagaat atggatcctg gttcTCCAGG 721 TCAGACAGAG GGTGAAACCT GACCGCAAAC CCAAACTGAA ACCACAGGAC TTCAGGAATT 781 TTGTCACCAT GTTTGTGGTT TTTGTCCTTT TTGCCATTTG CTGGGCTCCT CTGAACTTCA 841 TTGGCCTGGC CGTGGCCTCT GACCCCGCCA GCATGGTGCC TAGGATCCCA GAGTGGCTGT 901 TTGTGGCCAG TTACTACATG GCGTATTTCA ACAGCTGCCT CAATGCCATT ATATACGGGC 961 TACTGAACCA AAATTTCAGG AAGGAATACA GGAGAATTAT AGTCTCGCTC TGTACAGCCA 1021 GGGTGTTCTT TGTGGACAGC TCTAACGACG TGGCCGATAG GGTTAAATGG AAACCGTCTC 1081 CACTGATGAC CAACAATAAT GTAGTAAAGG TGGACTCCGT TTAAGACCCA GCTTTCTTGT 1141 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1201 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1261 GAAAGGACGA AATGGCAGCT TGACTTCAAT GCACACGCGT TAAGTCgaca atcaacctct 1321 ggattacaaa atttgtgaaa gatt