Construct: ORF TRCN0000492324
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010439.1_s317c1
- Derived from:
- ccsbBroadEn_07998
- DNA Barcode:
- AAATACGGAGCCGCTGTGTGCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGS22 (26166)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492324
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26166 | RGS22 | regulator of G protein sign... | NM_015668.5 | 99.9% | 99.8% | 1132G>A;3351A>G;3775G>T |
| 2 | human | 26166 | RGS22 | regulator of G protein sign... | NM_001286692.2 | 98.9% | 98.8% | (many diffs) |
| 3 | human | 26166 | RGS22 | regulator of G protein sign... | XM_005250856.3 | 95.7% | 95.6% | (many diffs) |
| 4 | human | 26166 | RGS22 | regulator of G protein sign... | XM_005250857.3 | 95.7% | 95.6% | (many diffs) |
| 5 | human | 26166 | RGS22 | regulator of G protein sign... | XM_005250858.3 | 95.6% | 95.5% | (many diffs) |
| 6 | human | 26166 | RGS22 | regulator of G protein sign... | XM_006716540.2 | 92.3% | 92.2% | (many diffs) |
| 7 | human | 26166 | RGS22 | regulator of G protein sign... | XM_011516957.1 | 92.3% | 92.2% | (many diffs) |
| 8 | human | 26166 | RGS22 | regulator of G protein sign... | XM_011516958.1 | 92.3% | 92.2% | (many diffs) |
| 9 | human | 26166 | RGS22 | regulator of G protein sign... | XM_017013309.2 | 91.5% | 91.4% | (many diffs) |
| 10 | human | 26166 | RGS22 | regulator of G protein sign... | XM_017013310.2 | 91.4% | 91.3% | (many diffs) |
| 11 | human | 26166 | RGS22 | regulator of G protein sign... | NM_001286693.2 | 85.6% | 85.5% | (many diffs) |
| 12 | human | 26166 | RGS22 | regulator of G protein sign... | XM_005250860.3 | 64.9% | 64.6% | (many diffs) |
| 13 | human | 26166 | RGS22 | regulator of G protein sign... | XM_017013311.1 | 63.9% | 63.9% | 0_1ins1365;1986A>G;2410G>T |
| 14 | human | 26166 | RGS22 | regulator of G protein sign... | XM_011516959.3 | 57.8% | 57.2% | (many diffs) |
| 15 | human | 26166 | RGS22 | regulator of G protein sign... | XM_024447121.1 | 51.6% | 51.5% | 0_1ins1833;1518A>G;1942G>T |
| 16 | human | 26166 | RGS22 | regulator of G protein sign... | XM_024447122.1 | 51.6% | 51.5% | 0_1ins1833;1518A>G;1942G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 68
- ORF end:
- 3860
- ORF length:
- 3792
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggaccatg cccgagaaga ggctcaccgc ggagccacca actattacag aagaagaatt 121 tgaagattct ctggcaacag atgatttcct tgtagactac tttaatgaat tcctaagcct 181 tccaaccttt tcagaggcaa ttagatttaa tgcagattat ggagtttttg aagtagctaa 241 tgatgctcca caatttctgg aaaaacaact gaaaaaaatt ctacaaaacc agcaacctcg 301 aaatcccatt tatgatgttg taaggaaagg aaagaatgag gttaaacctg ttcaaatgaa 361 tgcccccgat gaagatgaga ccattaatgt caactacaat attatgtgtc tcagtcgtga 421 agaaggtatt aagtggatca aaaaagaaag acttccagca tttctggaaa gtgattgtta 481 ctttgaatac aggttagcca aattggtctc acaagtaaga tggagcaagt ccggcatgaa 541 tttcacagta ggatcaaatt tttctccctg gatcgtgaaa aaaccaccca gtctaccacc 601 tcctgccact gaagaagata atcttgtaat tatgaaaaag ttctatgtat cacttggtga 661 ggcttcctat actcaaacaa aagattggtt tgcattagca aaacaaagtc agcaaacagt 721 atcaaccttt tcgttaccct gttgtgtacc ctacaacaaa cttaaatcac cagctatttc 781 atctgtttct gagaatttta tctttgatga tggagttcac cctaggacaa aaaaggaccc 841 atctaaaacc aacaaattga tttctgaatt tgaagaagaa gaaggagaag aagaagaggt 901 gtctgtatct ctacaagaca ctccttctca agctcttctg agagtatacc ttgaaaagaa 961 acaggatgtt gatgaaagcc tgacaatgca tttctcaaca tgtgaagaat ttttaagctc 1021 ctatatatac tttattttga gaggagcaat tcagcaaatt gttggaaagc cagttggaga 1081 aaccccagac tatataaact tcaacaatat aacaaaagtg tcatttgatg attgttttga 1141 gtctattcat ggcaagaatt ttttaagtga attagttcaa actacaaagg agaggtcaaa 1201 agagatagaa caaacaagtt taagttcaaa gaatgagagc gctggaccag agagcagggc 1261 ggactggtgt atttctcata ggacttatga cattggcaat agaaaggagt ttgaaagatt 1321 taagaaattt ataaaaggta cattgggaga gagatattgg tggctatgga tggatattga 1381 gaggttaaaa gttcttaagg atcctggaag acatcaaaga catcttgaga agatgaaaaa 1441 atgctatcta gtgagcaatg gagattacta cctttctgca gaaatcttat caaaatttaa 1501 actgttagat ggatcacagt ggaatgaaga gcatttaaga aatattcagt ctgaagtttt 1561 aaaaccttta cttctgtatt gggcaccaag attctgtgtt actcattcag cctcaacaaa 1621 atatgctagt gctgaactga aattctggca cctccgtcaa gcaaaaccaa ggaaggatat 1681 tgaccctttt ccacaaatgg caaccctctt gccattaaga cccaaatctt gcattccaca 1741 gatacctgag atccagaaag aagaattcag cttatcacaa cctcctaaat ctcccaataa 1801 atcacctgaa gtaaaaacag caactcaaaa gccttggaag cgggagcttt tgtatccagg 1861 ttcttctaag gatgatgtga ttgagaaagg gtcaaagtac atgtcagaaa gcagcaaagt 1921 cattcattta acatctttta ctgacatttc tgaatgtctc aagccccagc tggatagaag 1981 atacgcttat acagaagaac ctagggttaa aaccgtgtca gatgttggtg ccttgggagg 2041 atctgacatg gaaaatttgt tgcaatcttt gtatgtagaa aatagagctg gattcttctt 2101 tactaaattc tgcgagcatt cagggaacaa gttgtggaag aacagtgtgt acttttggtt 2161 tgacctgcag gcttatcatc agcttttcta ccaagaaaca cttcagcctt ttaaagtatg 2221 caagcaagcg caatatcttt ttgccacata cgttgctcct tctgccactc ttgacattgg 2281 actccaacag gaaaagaaaa aagaaattta tatgaaaata cagccaccat ttgaagacct 2341 ttttgacaca gcagaggaat atatccttct cctccttctt gagccatgga caaagatggt 2401 aaaatcggac caaattgctt ataaaaaggt ggagctggtg gaagaaactc gacagttaga 2461 ctccacatac ttcagaaagc tacaggcttt gcataaagag acattttcca agaaagctga 2521 ggacaccact tgtgaaattg gaactggcat tttatcactc tctaacgtct ctaaacgaac 2581 agaatattgg gataatgttc ctgcagaata caagcatttt aagtttagtg atctgcttaa 2641 caacaaactg gagtttgaac atttccgtca gtttcttgag actcattctt caagcatgga 2701 tcttatgtgc tggacagaca ttgagcagtt ccggagaata acttacagag atcgaaatca 2761 aaggaaggca aaatctatat acattaaaaa caaatacctt aataaaaaat atttctttgg 2821 acccaacagt ccagcttctc tgtatcagca gaaccaggta atgcatttaa gtggtggctg 2881 ggggaagatt cttcatgagc agcttgatgc acctgttcta gttgaaatcc agaagcatgt 2941 gcaaaatagg ctagaaaatg tatggctgcc attgtttctt gcaagtgaac agtttgcagc 3001 acgtcagaag ataaaggtac agatgaaaga catagcagaa gagctcttac tacagaaggc 3061 tgaaaagaaa atcggggtct ggaagcctgt ggagagtaag tggatctctt catcttgtaa 3121 aatcattgct tttcgcaaag cattattgaa tccagttact tcaagacaat ttcaacgttt 3181 tgtggctcta aaaggagatt tattggaaaa tggtttactc ttttggcaag aagtacaaaa 3241 atataaggac ttgtgccatt ctcattgtga tgagtctgtc atccagaaga agattacaac 3301 tattatcaac tgctttatta attccagtat tccaccagct ttacaaattg acattccagt 3361 agagcaagcc cagaagatta ttgaacaccg gaaggagtta ggaCCATATG TATTTAGGGA 3421 GGCACAGATG ACAATTTTTG GGGTTCTGTT TAAATTCTGG CCTCAGTTCT GTGAGTTTAG 3481 GAAGAATTTA ACAGATGAAA ATATTATGAG TGTTTTAGAG AGAAGACAAG AATATAATAA 3541 GCAGAAAAAA AAATTGGCAG TCCTAGAAGA CGAAAAATCT GGAAAGGATG GAATCAAACA 3601 ATATGCAAAT ACTTCAGTGC CTGCTATCAA AACTGCTTTA CTCAGTGATT CCTTCCTAGG 3661 CCTCCAACCA TATGGCCGAC AGCCAACCTG GTGCTACTCA AAGTATATAG AAGCCTTAGA 3721 ACAGGAGAGA ATTCTTCTTA AGATCCAAGA AGAACTAGAA AAAAAGTTGT TTGCAGGCTT 3781 GCAACCTCTC ACAAATTTTA AGGCTAGCTC TTCAACTATG TCTTTGAAAA AGAATATGTC 3841 TTCCCACAGC AGCCAGAAGT TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 3901 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 3961 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAATA CGGAGCCGCT 4021 GTGTGCGCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt