Construct: ORF TRCN0000492324

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010439.1_s317c1
Derived from:
ccsbBroadEn_07998
DNA Barcode:
AAATACGGAGCCGCTGTGTGCGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RGS22 (26166)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492324
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26166 RGS22 regulator of G protein sign... NM_015668.5 99.9% 99.8% 1132G>A;3351A>G;3775G>T
2 human 26166 RGS22 regulator of G protein sign... NM_001286692.2 98.9% 98.8% (many diffs)
3 human 26166 RGS22 regulator of G protein sign... XM_005250856.3 95.7% 95.6% (many diffs)
4 human 26166 RGS22 regulator of G protein sign... XM_005250857.3 95.7% 95.6% (many diffs)
5 human 26166 RGS22 regulator of G protein sign... XM_005250858.3 95.6% 95.5% (many diffs)
6 human 26166 RGS22 regulator of G protein sign... XM_006716540.2 92.3% 92.2% (many diffs)
7 human 26166 RGS22 regulator of G protein sign... XM_011516957.1 92.3% 92.2% (many diffs)
8 human 26166 RGS22 regulator of G protein sign... XM_011516958.1 92.3% 92.2% (many diffs)
9 human 26166 RGS22 regulator of G protein sign... XM_017013309.2 91.5% 91.4% (many diffs)
10 human 26166 RGS22 regulator of G protein sign... XM_017013310.2 91.4% 91.3% (many diffs)
11 human 26166 RGS22 regulator of G protein sign... NM_001286693.2 85.6% 85.5% (many diffs)
12 human 26166 RGS22 regulator of G protein sign... XM_005250860.3 64.9% 64.6% (many diffs)
13 human 26166 RGS22 regulator of G protein sign... XM_017013311.1 63.9% 63.9% 0_1ins1365;1986A>G;2410G>T
14 human 26166 RGS22 regulator of G protein sign... XM_011516959.3 57.8% 57.2% (many diffs)
15 human 26166 RGS22 regulator of G protein sign... XM_024447121.1 51.6% 51.5% 0_1ins1833;1518A>G;1942G>T
16 human 26166 RGS22 regulator of G protein sign... XM_024447122.1 51.6% 51.5% 0_1ins1833;1518A>G;1942G>T
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
68
ORF end:
3860
ORF length:
3792
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggaccatg cccgagaaga ggctcaccgc ggagccacca actattacag aagaagaatt
121tgaagattct ctggcaacag atgatttcct tgtagactac tttaatgaat tcctaagcct
181tccaaccttt tcagaggcaa ttagatttaa tgcagattat ggagtttttg aagtagctaa
241tgatgctcca caatttctgg aaaaacaact gaaaaaaatt ctacaaaacc agcaacctcg
301aaatcccatt tatgatgttg taaggaaagg aaagaatgag gttaaacctg ttcaaatgaa
361tgcccccgat gaagatgaga ccattaatgt caactacaat attatgtgtc tcagtcgtga
421agaaggtatt aagtggatca aaaaagaaag acttccagca tttctggaaa gtgattgtta
481ctttgaatac aggttagcca aattggtctc acaagtaaga tggagcaagt ccggcatgaa
541tttcacagta ggatcaaatt tttctccctg gatcgtgaaa aaaccaccca gtctaccacc
601tcctgccact gaagaagata atcttgtaat tatgaaaaag ttctatgtat cacttggtga
661ggcttcctat actcaaacaa aagattggtt tgcattagca aaacaaagtc agcaaacagt
721atcaaccttt tcgttaccct gttgtgtacc ctacaacaaa cttaaatcac cagctatttc
781atctgtttct gagaatttta tctttgatga tggagttcac cctaggacaa aaaaggaccc
841atctaaaacc aacaaattga tttctgaatt tgaagaagaa gaaggagaag aagaagaggt
901gtctgtatct ctacaagaca ctccttctca agctcttctg agagtatacc ttgaaaagaa
961acaggatgtt gatgaaagcc tgacaatgca tttctcaaca tgtgaagaat ttttaagctc
1021ctatatatac tttattttga gaggagcaat tcagcaaatt gttggaaagc cagttggaga
1081aaccccagac tatataaact tcaacaatat aacaaaagtg tcatttgatg attgttttga
1141gtctattcat ggcaagaatt ttttaagtga attagttcaa actacaaagg agaggtcaaa
1201agagatagaa caaacaagtt taagttcaaa gaatgagagc gctggaccag agagcagggc
1261ggactggtgt atttctcata ggacttatga cattggcaat agaaaggagt ttgaaagatt
1321taagaaattt ataaaaggta cattgggaga gagatattgg tggctatgga tggatattga
1381gaggttaaaa gttcttaagg atcctggaag acatcaaaga catcttgaga agatgaaaaa
1441atgctatcta gtgagcaatg gagattacta cctttctgca gaaatcttat caaaatttaa
1501actgttagat ggatcacagt ggaatgaaga gcatttaaga aatattcagt ctgaagtttt
1561aaaaccttta cttctgtatt gggcaccaag attctgtgtt actcattcag cctcaacaaa
1621atatgctagt gctgaactga aattctggca cctccgtcaa gcaaaaccaa ggaaggatat
1681tgaccctttt ccacaaatgg caaccctctt gccattaaga cccaaatctt gcattccaca
1741gatacctgag atccagaaag aagaattcag cttatcacaa cctcctaaat ctcccaataa
1801atcacctgaa gtaaaaacag caactcaaaa gccttggaag cgggagcttt tgtatccagg
1861ttcttctaag gatgatgtga ttgagaaagg gtcaaagtac atgtcagaaa gcagcaaagt
1921cattcattta acatctttta ctgacatttc tgaatgtctc aagccccagc tggatagaag
1981atacgcttat acagaagaac ctagggttaa aaccgtgtca gatgttggtg ccttgggagg
2041atctgacatg gaaaatttgt tgcaatcttt gtatgtagaa aatagagctg gattcttctt
2101tactaaattc tgcgagcatt cagggaacaa gttgtggaag aacagtgtgt acttttggtt
2161tgacctgcag gcttatcatc agcttttcta ccaagaaaca cttcagcctt ttaaagtatg
2221caagcaagcg caatatcttt ttgccacata cgttgctcct tctgccactc ttgacattgg
2281actccaacag gaaaagaaaa aagaaattta tatgaaaata cagccaccat ttgaagacct
2341ttttgacaca gcagaggaat atatccttct cctccttctt gagccatgga caaagatggt
2401aaaatcggac caaattgctt ataaaaaggt ggagctggtg gaagaaactc gacagttaga
2461ctccacatac ttcagaaagc tacaggcttt gcataaagag acattttcca agaaagctga
2521ggacaccact tgtgaaattg gaactggcat tttatcactc tctaacgtct ctaaacgaac
2581agaatattgg gataatgttc ctgcagaata caagcatttt aagtttagtg atctgcttaa
2641caacaaactg gagtttgaac atttccgtca gtttcttgag actcattctt caagcatgga
2701tcttatgtgc tggacagaca ttgagcagtt ccggagaata acttacagag atcgaaatca
2761aaggaaggca aaatctatat acattaaaaa caaatacctt aataaaaaat atttctttgg
2821acccaacagt ccagcttctc tgtatcagca gaaccaggta atgcatttaa gtggtggctg
2881ggggaagatt cttcatgagc agcttgatgc acctgttcta gttgaaatcc agaagcatgt
2941gcaaaatagg ctagaaaatg tatggctgcc attgtttctt gcaagtgaac agtttgcagc
3001acgtcagaag ataaaggtac agatgaaaga catagcagaa gagctcttac tacagaaggc
3061tgaaaagaaa atcggggtct ggaagcctgt ggagagtaag tggatctctt catcttgtaa
3121aatcattgct tttcgcaaag cattattgaa tccagttact tcaagacaat ttcaacgttt
3181tgtggctcta aaaggagatt tattggaaaa tggtttactc ttttggcaag aagtacaaaa
3241atataaggac ttgtgccatt ctcattgtga tgagtctgtc atccagaaga agattacaac
3301tattatcaac tgctttatta attccagtat tccaccagct ttacaaattg acattccagt
3361agagcaagcc cagaagatta ttgaacaccg gaaggagtta ggaCCATATG TATTTAGGGA
3421GGCACAGATG ACAATTTTTG GGGTTCTGTT TAAATTCTGG CCTCAGTTCT GTGAGTTTAG
3481GAAGAATTTA ACAGATGAAA ATATTATGAG TGTTTTAGAG AGAAGACAAG AATATAATAA
3541GCAGAAAAAA AAATTGGCAG TCCTAGAAGA CGAAAAATCT GGAAAGGATG GAATCAAACA
3601ATATGCAAAT ACTTCAGTGC CTGCTATCAA AACTGCTTTA CTCAGTGATT CCTTCCTAGG
3661CCTCCAACCA TATGGCCGAC AGCCAACCTG GTGCTACTCA AAGTATATAG AAGCCTTAGA
3721ACAGGAGAGA ATTCTTCTTA AGATCCAAGA AGAACTAGAA AAAAAGTTGT TTGCAGGCTT
3781GCAACCTCTC ACAAATTTTA AGGCTAGCTC TTCAACTATG TCTTTGAAAA AGAATATGTC
3841TTCCCACAGC AGCCAGAAGT TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC
3901TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA
3961AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAATA CGGAGCCGCT
4021GTGTGCGCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)

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