Construct: ORF TRCN0000492327

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021679.2_s317c1
DNA Barcode:
TAGTGGACTAGGTAGTCAATGGTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GRIN2A (2903)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492327
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2903 GRIN2A glutamate ionotropic recept... NM_000833.5 100% 100%
2 human 2903 GRIN2A glutamate ionotropic recept... NM_001134407.3 100% 100%
3 human 2903 GRIN2A glutamate ionotropic recept... XM_017023172.1 96.5% 96.5% 1_156del
4 human 2903 GRIN2A glutamate ionotropic recept... XM_011522458.3 89.2% 89.2% 0_1ins471
5 human 2903 GRIN2A glutamate ionotropic recept... NM_001134408.2 87.5% 86.4% 3768_3769ins343;3843_3844ins206
6 human 2903 GRIN2A glutamate ionotropic recept... XM_011522461.3 87.5% 86.4% 3768_3769ins343;3843_3844ins206
7 human 2903 GRIN2A glutamate ionotropic recept... XM_017023173.1 84.4% 83.5% 1_156del;3924_3925ins343;3999_4000ins206
8 mouse 14811 Grin2a glutamate receptor, ionotro... NM_008170.2 88.8% 95.2% (many diffs)
9 mouse 14811 Grin2a glutamate receptor, ionotro... XM_006521794.3 88.8% 95.2% (many diffs)
10 mouse 14811 Grin2a glutamate receptor, ionotro... XM_006521795.3 43.9% 48% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4461
ORF length:
4392
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gggcagagtg ggctattgga ccctgctggt gctgccggcc cttctggtct
121ggcgcggtcc ggcgccgagc gcggcggcgg agaagggtcc ccccgcgcta aatattgcgg
181tgatgctggg tcacagccac gacgtgacag agcgcgaact tcgaacactg tggggccccg
241agcaggcggc ggggctgccc ctggacgtga acgtggtagc tctgctgatg aaccgcaccg
301accccaagag cctcatcacg cacgtgtgcg acctcatgtc cggggcacgc atccacggcc
361tcgtgtttgg ggacgacacg gaccaggagg ccgtagccca gatgctggat tttatctcct
421cccacacctt cgtccccatc ttgggcattc atgggggcgc atctatgatc atggctgaca
481aggatccgac gtctaccttc ttccagtttg gagcgtccat ccagcagcaa gccacggtca
541tgctgaagat catgcaggat tatgactggc atgtcttctc cctggtgacc actatcttcc
601ctggctacag ggaattcatc agcttcgtca agaccacagt ggacaacagc tttgtgggct
661gggacatgca gaatgtgatc acactggaca cttcctttga ggatgcaaag acacaagtcc
721agctgaagaa gatccactct tctgtcatct tgctctactg ttccaaagac gaggctgttc
781tcattctgag tgaggcccgc tcccttggcc tcaccgggta tgatttcttc tggattgtcc
841ccagcttggt ctctgggaac acggagctca tcccaaaaga gtttccatcg ggactcattt
901ctgtctccta cgatgactgg gactacagcc tggaggcgag agtgagggac ggcattggca
961tcctaaccac cgctgcatct tctatgctgg agaagttctc ctacatcccc gaggccaagg
1021ccagctgcta cgggcagatg gagaggccag aggtcccgat gcacaccttg cacccattta
1081tggtcaatgt tacatgggat ggcaaagact tatccttcac tgaggaaggc taccaggtgc
1141accccaggct ggtggtgatt gtgctgaaca aagaccggga atgggaaaag gtgggcaagt
1201gggagaacca tacgctgagc ctgaggcacg ccgtgtggcc caggtacaag tccttctccg
1261actgtgagcc ggatgacaac catctcagca tcgtcaccct ggaggaggcc ccattcgtca
1321tcgtggaaga catagacccc ctgaccgaga cgtgtgtgag gaacaccgtg ccatgtcgga
1381agttcgtcaa aatcaacaat tcaaccaatg aggggatgaa tgtgaagaaa tgctgcaagg
1441ggttctgcat tgatattctg aagaagcttt ccagaactgt gaagtttact tacgacctct
1501atctggtgac caatgggaag catggcaaga aagttaacaa tgtgtggaat ggaatgatcg
1561gtgaagtggt ctatcaacgg gcagtcatgg cagttggctc gctcaccatc aatgaggaac
1621gttctgaagt ggtggacttc tctgtgccct ttgtggaaac gggaatcagt gtcatggttt
1681caagaagtaa tggcaccgtc tcaccttctg cttttctaga accattcagc gcctctgtct
1741gggtgatgat gtttgtgatg ctgctcattg tttctgccat agctgttttt gtctttgaat
1801acttcagccc tgttggatac aacagaaact tagccaaagg gaaagcaccc catgggcctt
1861cttttacaat tggaaaagct atatggcttc tttggggcct ggtgttcaat aactccgtgc
1921ctgtccagaa tcctaaaggg accaccagca agatcatggt atctgtatgg gccttcttcg
1981ctgtcatatt cctggctagc tacacagcca atctggctgc cttcatgatc caagaggaat
2041ttgtggacca agtgaccggc ctcagtgaca aaaagtttca gagacctcat gactattccc
2101caccttttcg atttgggaca gtgcctaatg gaagcacgga gagaaacatt cggaataact
2161atccctacat gcatcagtac atgaccaaat ttaatcagaa aggagtagag gacgccttgg
2221tcagcctgaa aacggggaag ctggacgctt tcatctacga tgccgcagtc ttgaattaca
2281aggctgggag ggatgaaggc tgcaagctgg tgaccatcgg gagtgggtac atctttgcca
2341ccaccggtta tggaattgcc cttcagaaag gctctccttg gaagaggcag atcgacctgg
2401ccttgcttca gtttgtgggt gatggtgaga tggaggagct ggagaccctg tggctcactg
2461ggatctgcca caacgagaag aacgaggtga tgagcagcca gctggacatt gacaacatgg
2521cgggcgtatt ctacatgctg gctgccgcca tggcccttag cctcatcacc ttcatctggg
2581agcacctctt ctactggaag ctgcgcttct gtttcacggg cgtgtgctcc gaccggcctg
2641ggttgctctt ctccatcagc aggggcatct acagctgcat tcatggagtg cacattgaag
2701aaaagaagaa gtctccagac ttcaatctga cgggatccca gagcaacatg ttaaaactcc
2761tccggtcagc caaaaacatt tccagcatgt ccaacatgaa ctcctcaaga atggactcac
2821ccaaaagagc tgctgacttc atccaaagag gttccctcat catggacatg gtttcagata
2881aggggaattt gatgtactca gacaacaggt cctttcaggg gaaagagagc atttttggag
2941acaacatgaa cgaactccaa acatttgtgg ccaaccggca gaaggataac ctcaataact
3001atgtattcca gggacaacat cctcttactc tcaatgagtc caaccctaac acggtggagg
3061tggccgtgag cacagaatcc aaagcgaact ctagaccccg gcagctgtgg aagaaatccg
3121tggattccat acgccaggat tcactatccc agaatccagt ctcccagagg gatgaggcaa
3181cagcagagaa taggacccac tccctaaaga gccctaggta tcttccagaa gagatggccc
3241actctgacat ttcagaaacg tcaaatcggg ccacgtgcca cagggaacct gacaacagta
3301agaaccacaa aaccaaggac aactttaaaa ggtcagtggc ctccaaatac cccaaggact
3361gtagtgaggt cgagcgcacc tacctgaaaa ccaaatcaag ctcccctaga gacaagatct
3421acactataga tggtgagaag gagcctggtt tccacttaga tccaccccag tttgttgaaa
3481atgtgaccct gcccgagaac gtggacttcc cggaccccta ccaggatccc agtgaaaact
3541tccgcaaggg ggactccacg ctgccaatga accggaaccc cttgcataat gaagaggggc
3601tttccaacaa cgaccagtat aaactctact ccaagcactt caccttgaaa gacaagggtt
3661ccccgcacag tgagaccagc gagcgatacc ggcagaactc cacgcactgc agaagctgcc
3721tttccaacat gcccacctat tcaggccact tcaccatgag gtcccccttc aagtgcgatg
3781cctgcctgcg gatggggaac ctctatgaca tcgatgaaga ccagatgctt caggagacag
3841gtaacccagc caccggggag caggtctacc agcaggactg ggcacagaac aatgcccttc
3901aattacaaaa gaacaagcta aggattagcc gtcagcattc ctacgataac attgtcgaca
3961aacctaggga gctagacctt agcaggccct cccggagcat aagcctcaag gacagggaac
4021ggcttctgga gggaaatttt tacggcagcc tgtTTAGTGT CCCCTCAAGC AAACTCTCGG
4081GGAAAAAAAG CTCCCTTTTC CCCCAAGGTC TGGAGGACAG CAAGAGGAGC AAGTCTCTCT
4141TGCCAGACCA CACCTCCGAT AACCCTTTCC TCCACTCCCA CAGGGATGAC CAACGCTTGG
4201TTATTGGGAG ATGCCCCTCG GACCCTTACA AACACTCGTT GCCATCCCAG GCGGTGAATG
4261ACAGCTATCT TCGGTCGTCC TTGAGGTCAA CGGCATCGTA CTGTTCCAGG GACAGTCGGG
4321GCCACAATGA TGTGTATATT TCGGAGCATG TTATGCCTTA TGCTGCAAAT AAGAATAATA
4381TGTACTCTAC CCCCAGGGTT TTAAATTCCT GCAGCAATAG ACGCGTGTAC AAGAAAATGC
4441CTAGTATCGA ATCTGATGTT TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA
4501AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
4561TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AGTGGACTAG
4621GTAGTCAATG GTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
4681att

Download FASTA (ORF) (Full)