Construct: ORF TRCN0000492327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021679.2_s317c1
- DNA Barcode:
- TAGTGGACTAGGTAGTCAATGGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRIN2A (2903)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492327
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2903 | GRIN2A | glutamate ionotropic recept... | NM_000833.5 | 100% | 100% | |
| 2 | human | 2903 | GRIN2A | glutamate ionotropic recept... | NM_001134407.3 | 100% | 100% | |
| 3 | human | 2903 | GRIN2A | glutamate ionotropic recept... | XM_017023172.1 | 96.5% | 96.5% | 1_156del |
| 4 | human | 2903 | GRIN2A | glutamate ionotropic recept... | XM_011522458.3 | 89.2% | 89.2% | 0_1ins471 |
| 5 | human | 2903 | GRIN2A | glutamate ionotropic recept... | NM_001134408.2 | 87.5% | 86.4% | 3768_3769ins343;3843_3844ins206 |
| 6 | human | 2903 | GRIN2A | glutamate ionotropic recept... | XM_011522461.3 | 87.5% | 86.4% | 3768_3769ins343;3843_3844ins206 |
| 7 | human | 2903 | GRIN2A | glutamate ionotropic recept... | XM_017023173.1 | 84.4% | 83.5% | 1_156del;3924_3925ins343;3999_4000ins206 |
| 8 | mouse | 14811 | Grin2a | glutamate receptor, ionotro... | NM_008170.2 | 88.8% | 95.2% | (many diffs) |
| 9 | mouse | 14811 | Grin2a | glutamate receptor, ionotro... | XM_006521794.3 | 88.8% | 95.2% | (many diffs) |
| 10 | mouse | 14811 | Grin2a | glutamate receptor, ionotro... | XM_006521795.3 | 43.9% | 48% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4461
- ORF length:
- 4392
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggcagagtg ggctattgga ccctgctggt gctgccggcc cttctggtct 121 ggcgcggtcc ggcgccgagc gcggcggcgg agaagggtcc ccccgcgcta aatattgcgg 181 tgatgctggg tcacagccac gacgtgacag agcgcgaact tcgaacactg tggggccccg 241 agcaggcggc ggggctgccc ctggacgtga acgtggtagc tctgctgatg aaccgcaccg 301 accccaagag cctcatcacg cacgtgtgcg acctcatgtc cggggcacgc atccacggcc 361 tcgtgtttgg ggacgacacg gaccaggagg ccgtagccca gatgctggat tttatctcct 421 cccacacctt cgtccccatc ttgggcattc atgggggcgc atctatgatc atggctgaca 481 aggatccgac gtctaccttc ttccagtttg gagcgtccat ccagcagcaa gccacggtca 541 tgctgaagat catgcaggat tatgactggc atgtcttctc cctggtgacc actatcttcc 601 ctggctacag ggaattcatc agcttcgtca agaccacagt ggacaacagc tttgtgggct 661 gggacatgca gaatgtgatc acactggaca cttcctttga ggatgcaaag acacaagtcc 721 agctgaagaa gatccactct tctgtcatct tgctctactg ttccaaagac gaggctgttc 781 tcattctgag tgaggcccgc tcccttggcc tcaccgggta tgatttcttc tggattgtcc 841 ccagcttggt ctctgggaac acggagctca tcccaaaaga gtttccatcg ggactcattt 901 ctgtctccta cgatgactgg gactacagcc tggaggcgag agtgagggac ggcattggca 961 tcctaaccac cgctgcatct tctatgctgg agaagttctc ctacatcccc gaggccaagg 1021 ccagctgcta cgggcagatg gagaggccag aggtcccgat gcacaccttg cacccattta 1081 tggtcaatgt tacatgggat ggcaaagact tatccttcac tgaggaaggc taccaggtgc 1141 accccaggct ggtggtgatt gtgctgaaca aagaccggga atgggaaaag gtgggcaagt 1201 gggagaacca tacgctgagc ctgaggcacg ccgtgtggcc caggtacaag tccttctccg 1261 actgtgagcc ggatgacaac catctcagca tcgtcaccct ggaggaggcc ccattcgtca 1321 tcgtggaaga catagacccc ctgaccgaga cgtgtgtgag gaacaccgtg ccatgtcgga 1381 agttcgtcaa aatcaacaat tcaaccaatg aggggatgaa tgtgaagaaa tgctgcaagg 1441 ggttctgcat tgatattctg aagaagcttt ccagaactgt gaagtttact tacgacctct 1501 atctggtgac caatgggaag catggcaaga aagttaacaa tgtgtggaat ggaatgatcg 1561 gtgaagtggt ctatcaacgg gcagtcatgg cagttggctc gctcaccatc aatgaggaac 1621 gttctgaagt ggtggacttc tctgtgccct ttgtggaaac gggaatcagt gtcatggttt 1681 caagaagtaa tggcaccgtc tcaccttctg cttttctaga accattcagc gcctctgtct 1741 gggtgatgat gtttgtgatg ctgctcattg tttctgccat agctgttttt gtctttgaat 1801 acttcagccc tgttggatac aacagaaact tagccaaagg gaaagcaccc catgggcctt 1861 cttttacaat tggaaaagct atatggcttc tttggggcct ggtgttcaat aactccgtgc 1921 ctgtccagaa tcctaaaggg accaccagca agatcatggt atctgtatgg gccttcttcg 1981 ctgtcatatt cctggctagc tacacagcca atctggctgc cttcatgatc caagaggaat 2041 ttgtggacca agtgaccggc ctcagtgaca aaaagtttca gagacctcat gactattccc 2101 caccttttcg atttgggaca gtgcctaatg gaagcacgga gagaaacatt cggaataact 2161 atccctacat gcatcagtac atgaccaaat ttaatcagaa aggagtagag gacgccttgg 2221 tcagcctgaa aacggggaag ctggacgctt tcatctacga tgccgcagtc ttgaattaca 2281 aggctgggag ggatgaaggc tgcaagctgg tgaccatcgg gagtgggtac atctttgcca 2341 ccaccggtta tggaattgcc cttcagaaag gctctccttg gaagaggcag atcgacctgg 2401 ccttgcttca gtttgtgggt gatggtgaga tggaggagct ggagaccctg tggctcactg 2461 ggatctgcca caacgagaag aacgaggtga tgagcagcca gctggacatt gacaacatgg 2521 cgggcgtatt ctacatgctg gctgccgcca tggcccttag cctcatcacc ttcatctggg 2581 agcacctctt ctactggaag ctgcgcttct gtttcacggg cgtgtgctcc gaccggcctg 2641 ggttgctctt ctccatcagc aggggcatct acagctgcat tcatggagtg cacattgaag 2701 aaaagaagaa gtctccagac ttcaatctga cgggatccca gagcaacatg ttaaaactcc 2761 tccggtcagc caaaaacatt tccagcatgt ccaacatgaa ctcctcaaga atggactcac 2821 ccaaaagagc tgctgacttc atccaaagag gttccctcat catggacatg gtttcagata 2881 aggggaattt gatgtactca gacaacaggt cctttcaggg gaaagagagc atttttggag 2941 acaacatgaa cgaactccaa acatttgtgg ccaaccggca gaaggataac ctcaataact 3001 atgtattcca gggacaacat cctcttactc tcaatgagtc caaccctaac acggtggagg 3061 tggccgtgag cacagaatcc aaagcgaact ctagaccccg gcagctgtgg aagaaatccg 3121 tggattccat acgccaggat tcactatccc agaatccagt ctcccagagg gatgaggcaa 3181 cagcagagaa taggacccac tccctaaaga gccctaggta tcttccagaa gagatggccc 3241 actctgacat ttcagaaacg tcaaatcggg ccacgtgcca cagggaacct gacaacagta 3301 agaaccacaa aaccaaggac aactttaaaa ggtcagtggc ctccaaatac cccaaggact 3361 gtagtgaggt cgagcgcacc tacctgaaaa ccaaatcaag ctcccctaga gacaagatct 3421 acactataga tggtgagaag gagcctggtt tccacttaga tccaccccag tttgttgaaa 3481 atgtgaccct gcccgagaac gtggacttcc cggaccccta ccaggatccc agtgaaaact 3541 tccgcaaggg ggactccacg ctgccaatga accggaaccc cttgcataat gaagaggggc 3601 tttccaacaa cgaccagtat aaactctact ccaagcactt caccttgaaa gacaagggtt 3661 ccccgcacag tgagaccagc gagcgatacc ggcagaactc cacgcactgc agaagctgcc 3721 tttccaacat gcccacctat tcaggccact tcaccatgag gtcccccttc aagtgcgatg 3781 cctgcctgcg gatggggaac ctctatgaca tcgatgaaga ccagatgctt caggagacag 3841 gtaacccagc caccggggag caggtctacc agcaggactg ggcacagaac aatgcccttc 3901 aattacaaaa gaacaagcta aggattagcc gtcagcattc ctacgataac attgtcgaca 3961 aacctaggga gctagacctt agcaggccct cccggagcat aagcctcaag gacagggaac 4021 ggcttctgga gggaaatttt tacggcagcc tgtTTAGTGT CCCCTCAAGC AAACTCTCGG 4081 GGAAAAAAAG CTCCCTTTTC CCCCAAGGTC TGGAGGACAG CAAGAGGAGC AAGTCTCTCT 4141 TGCCAGACCA CACCTCCGAT AACCCTTTCC TCCACTCCCA CAGGGATGAC CAACGCTTGG 4201 TTATTGGGAG ATGCCCCTCG GACCCTTACA AACACTCGTT GCCATCCCAG GCGGTGAATG 4261 ACAGCTATCT TCGGTCGTCC TTGAGGTCAA CGGCATCGTA CTGTTCCAGG GACAGTCGGG 4321 GCCACAATGA TGTGTATATT TCGGAGCATG TTATGCCTTA TGCTGCAAAT AAGAATAATA 4381 TGTACTCTAC CCCCAGGGTT TTAAATTCCT GCAGCAATAG ACGCGTGTAC AAGAAAATGC 4441 CTAGTATCGA ATCTGATGTT TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 4501 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 4561 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AGTGGACTAG 4621 GTAGTCAATG GTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 4681 att