Construct: ORF TRCN0000492332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019801.2_s317c1
- DNA Barcode:
- GTGTTATTTGGCCTAAGAGCTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAP2K1 (5604)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492332
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5604 | MAP2K1 | mitogen-activated protein k... | NM_002755.3 | 99.9% | 99.7% | 1179_1180insG |
| 2 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_011521783.3 | 93.6% | 92.8% | (many diffs) |
| 3 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_017022411.2 | 93.3% | 93.1% | 438_439ins78;1101_1102insG |
| 4 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_017022412.1 | 87% | 86.2% | (many diffs) |
| 5 | human | 5604 | MAP2K1 | mitogen-activated protein k... | XM_017022413.1 | 53.3% | 51.5% | (many diffs) |
| 6 | mouse | 26395 | Map2k1 | mitogen-activated protein k... | NM_008927.3 | 90.5% | 98.7% | (many diffs) |
| 7 | mouse | 26395 | Map2k1 | mitogen-activated protein k... | XM_006511196.3 | 84.7% | 91.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1251
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcccaagaag aagccgacgc ccatccagct gaacccggcc cccgacggct 121 ctgcagttaa cgggaccagc tctgcggaga ccaacttgga ggccttgcag aagaagctgg 181 aggagctaga gcttgatgag cagcagcgaa agcgccttga ggcctttctt acccagaagc 241 agaaggtggg agaactgaag gatgacgact ttgagaagat cagtgagctg ggggctggca 301 atggcggtgt ggtgttcaag gtctcccaca agccttctgg cctggtcatg gccagaaagc 361 taattcatct ggagatcaaa cccgcaatcc ggaaccagat cataagggag ctgcaggttc 421 tgcatgagtg caactctccg tacatcgtgg gcttctatgg tgcgttctac agcgatggcg 481 agatcagtat ctgcatggag cacatggatg gaggttctct ggatcaagtc ctgaagaaag 541 ctggaagaat tcctgaacaa attttaggaa aagttagcat tgctgtaata aaaggcctga 601 catatctgag ggagaagcac aagatcatgc acagagatgt caagccctcc aacatcctag 661 tcaactcccg tggggagatc aagctctgtg actttggggt cagcgggcag ctcatcgact 721 ccatggccaa ctccttcgtg ggcacaaggt cctacatgtc gccagaaaga ctccagggga 781 ctcattactc tgtgcagtca gacatctgga gcatgggact gtctctggta gagatggcgg 841 ttgggaggta tcccatcccT CCTCCAGATG CCAAGGAGCT GGAGCTGATG TTTGGGTGCC 901 AGGTGGAAGG AGATGCGGCT GAGACCCCAC CCAGGCCAAG GACCCCCGGG AGGCCCCTTA 961 GCTCATACGG AATGGACAGC CGACCTCCCA TGGCAATTTT TGAGTTGTTG GATTACATAG 1021 TCAACGAGCC TCCTCCAAAA CTGCCCAGTG GAGTGTTCAG TCTGGAATTT CAAGATTTTG 1081 TGAATAAATG CTTAATAAAA AACCCCGCAG AGAGAGCAGA TTTGAAGCAA CTCATGGTTC 1141 ATGCTTTTAT CAAGAGATCT GATGCTGAGG AAGTGGATTT TGCAGGTTGG CTCTGCTCCA 1201 CCATCGGCCT TAACCAGCCC AGCACACCAA CCCATGCTGC TGGCGTCGAC CCAGCTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGAGTGTTAT TTGGCCTAAG AGCTCACACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt