Construct: ORF TRCN0000492335
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020583.2_s317c1
- DNA Barcode:
- CGCCGGACCCGCAATACTGAATTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EEF2K (29904)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492335
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29904 | EEF2K | eukaryotic elongation facto... | NM_013302.4 | 99.9% | 100% | 489C>A |
| 2 | human | 29904 | EEF2K | eukaryotic elongation facto... | XM_017023193.2 | 99.9% | 99.8% | 489C>A;1082A>G |
| 3 | human | 29904 | EEF2K | eukaryotic elongation facto... | XM_017023194.2 | 97% | 96.9% | 489C>A;1082A>G;1377_1378ins63 |
| 4 | human | 29904 | EEF2K | eukaryotic elongation facto... | XR_950786.3 | 78.4% | (many diffs) | |
| 5 | human | 29904 | EEF2K | eukaryotic elongation facto... | XR_001751897.2 | 72.8% | (many diffs) | |
| 6 | human | 29904 | EEF2K | eukaryotic elongation facto... | XR_950787.3 | 51.7% | (many diffs) | |
| 7 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | NM_001267710.1 | 85.8% | 90% | (many diffs) |
| 8 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | NM_001267711.1 | 85.8% | 90% | (many diffs) |
| 9 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | NM_007908.4 | 85.8% | 90% | (many diffs) |
| 10 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507342.3 | 85.8% | 90% | (many diffs) |
| 11 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507343.3 | 85.8% | 90% | (many diffs) |
| 12 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507344.3 | 80.4% | 84.6% | (many diffs) |
| 13 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507345.3 | 72.9% | 76.5% | (many diffs) |
| 14 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XM_006507346.3 | 69.6% | 73.5% | (many diffs) |
| 15 | mouse | 13631 | Eef2k | eukaryotic elongation facto... | XR_378214.2 | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2247
- ORF length:
- 2175
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcagac gaagatctca tcttccgcct ggaaggcgtt gatggcggcc 121 agtccccccg agctggccat gatggtgatt ctgatgggga cagcgacgat gaggaaggtt 181 acttcatctg ccccatcacg gatgacccaa gctcgaacca gaatgtcaat tccaaggtta 241 ataagtacta cagcaaccta acaaaaagtg agcggtatag ctccagcggg tccccggcaa 301 actccttcca cttcaaggaa gcctggaagc acgcaatcca gaaggccaag cacatgcccg 361 acccctgggc tgagttccac ctggaagata ttgccaccga acgtgctact cgacacaggt 421 acaacgccgt caccggggaa tggctggatg atgaagttct gatcaagatg gcatctcagc 481 ccttcggccg aggagcaatg agggagtgct tccggacgaa gaagctctcc aacttcttgc 541 atgcccagca gtggaaggga gcctccaact acgtggcgaa gcgctacatc gagcccgtag 601 accgggatgt gtactttgag gacgtgcgtc tacagatgga ggccaagctc tggggggagg 661 agtataatcg gcacaagccc cccaagcagg tggacatcat gcagatgtgc atcatcgagc 721 tgaaggacag accgggcaag cccctcttcc acctggagca ctacatcgag ggcaagtaca 781 tcaagtacaa ctccaactct ggctttgtcc gcgatgacaa catccgcctg acgccgcagg 841 ccttcagcca cttcactttt gagcgttccg gccatcagct gatagtggtg gacatccagg 901 gagttgggga tctctacact gacccacaga tccacacgga gacgggcact gactttggag 961 acggcaacct aggtgtccgc gggatggcgc tcttcttcta ctctcatgcc tgcaaccgga 1021 tttgcgagag catgggcctt gctccctttg acctctcgcc ccgggagagg gatgcagtga 1081 atcagaacac caagctgctg caatcagcca agaccatctt gagaggaaca gaggaaaaat 1141 gtgggagccc ccgagtaagg accctctctg ggagccggcc acccctgctc cgtccccttt 1201 cagagaactc tggagacgag aacatgagcg acgtgacctt cgactctctc ccttcttccc 1261 catcttcggc cacaccacac agccagaagc tagaccacct ccattggcca gtgttcagtg 1321 acctcgataa catggcatcc agagaccatg atcatctaga caaccaccgg gagtctgaga 1381 atagtgggga cagcggatac cccagtgaga agcggggtga gctggatgac cctgagcccc 1441 gagaacatgg ccactcatac agtaatcgga agtacgagtc tgacgaagac agcctgggca 1501 gctctggacg ggtatgtgta gagaagtgga atctcctcaa ctcctcccgc ctccacctgc 1561 cgagggcttc ggccgtggcc ctggaagtgc aaaggcttaa tgctctggac ctcgaaaaga 1621 aaatcgggaa gtccattttg gggaaggtcc atctggccat ggtgcgctac cacgagggtg 1681 ggcgcttctg cgagaagggc gaggagtggg accaggagtc ggctgtcttc cacctggagc 1741 acgcagccaa cctgggcgag ctggaggcca tcgtgggcct gggactcatg tactcgcagt 1801 tgcctcatca catcctagcc gatgtctctc tgaaggagac agaagagaac aaaaccaaag 1861 gatttgatta cttactaaag gccgctgaag ctggcgacag gcagtccatg atcctagtgg 1921 cgcgagcttt tgactctggc cagaacctca gcccggacag gtgccaagac tggctagagg 1981 ccctgcactg gtacaacact gccctggaga tgacggactg tgatgagggc ggtgagtacg 2041 acggaatgca ggacgagccc cggtacatga tgctggccag ggaggccgag atgctgttca 2101 caggaggcta cgggctggag aaggacccgc agagatcagg ggacttgtat acccaggcag 2161 cagaggcagc gatggaagcc atgaagggcc gactggccaa ccagtactaC CAAAAGGCTG 2221 AAGAGGCCTG GGCCCAGATG GAGGAGTAAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 2281 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2341 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGCCG 2401 GACCCGCAAT ACTGAATTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2461 tgaaagatt