Construct: ORF TRCN0000492335

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020583.2_s317c1
DNA Barcode:
CGCCGGACCCGCAATACTGAATTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
EEF2K (29904)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492335
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29904 EEF2K eukaryotic elongation facto... NM_013302.4 99.9% 100% 489C>A
2 human 29904 EEF2K eukaryotic elongation facto... XM_017023193.2 99.9% 99.8% 489C>A;1082A>G
3 human 29904 EEF2K eukaryotic elongation facto... XM_017023194.2 97% 96.9% 489C>A;1082A>G;1377_1378ins63
4 human 29904 EEF2K eukaryotic elongation facto... XR_950786.3 78.4% (many diffs)
5 human 29904 EEF2K eukaryotic elongation facto... XR_001751897.2 72.8% (many diffs)
6 human 29904 EEF2K eukaryotic elongation facto... XR_950787.3 51.7% (many diffs)
7 mouse 13631 Eef2k eukaryotic elongation facto... NM_001267710.1 85.8% 90% (many diffs)
8 mouse 13631 Eef2k eukaryotic elongation facto... NM_001267711.1 85.8% 90% (many diffs)
9 mouse 13631 Eef2k eukaryotic elongation facto... NM_007908.4 85.8% 90% (many diffs)
10 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507342.3 85.8% 90% (many diffs)
11 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507343.3 85.8% 90% (many diffs)
12 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507344.3 80.4% 84.6% (many diffs)
13 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507345.3 72.9% 76.5% (many diffs)
14 mouse 13631 Eef2k eukaryotic elongation facto... XM_006507346.3 69.6% 73.5% (many diffs)
15 mouse 13631 Eef2k eukaryotic elongation facto... XR_378214.2 45.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2247
ORF length:
2175
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggcagac gaagatctca tcttccgcct ggaaggcgtt gatggcggcc
121agtccccccg agctggccat gatggtgatt ctgatgggga cagcgacgat gaggaaggtt
181acttcatctg ccccatcacg gatgacccaa gctcgaacca gaatgtcaat tccaaggtta
241ataagtacta cagcaaccta acaaaaagtg agcggtatag ctccagcggg tccccggcaa
301actccttcca cttcaaggaa gcctggaagc acgcaatcca gaaggccaag cacatgcccg
361acccctgggc tgagttccac ctggaagata ttgccaccga acgtgctact cgacacaggt
421acaacgccgt caccggggaa tggctggatg atgaagttct gatcaagatg gcatctcagc
481ccttcggccg aggagcaatg agggagtgct tccggacgaa gaagctctcc aacttcttgc
541atgcccagca gtggaaggga gcctccaact acgtggcgaa gcgctacatc gagcccgtag
601accgggatgt gtactttgag gacgtgcgtc tacagatgga ggccaagctc tggggggagg
661agtataatcg gcacaagccc cccaagcagg tggacatcat gcagatgtgc atcatcgagc
721tgaaggacag accgggcaag cccctcttcc acctggagca ctacatcgag ggcaagtaca
781tcaagtacaa ctccaactct ggctttgtcc gcgatgacaa catccgcctg acgccgcagg
841ccttcagcca cttcactttt gagcgttccg gccatcagct gatagtggtg gacatccagg
901gagttgggga tctctacact gacccacaga tccacacgga gacgggcact gactttggag
961acggcaacct aggtgtccgc gggatggcgc tcttcttcta ctctcatgcc tgcaaccgga
1021tttgcgagag catgggcctt gctccctttg acctctcgcc ccgggagagg gatgcagtga
1081atcagaacac caagctgctg caatcagcca agaccatctt gagaggaaca gaggaaaaat
1141gtgggagccc ccgagtaagg accctctctg ggagccggcc acccctgctc cgtccccttt
1201cagagaactc tggagacgag aacatgagcg acgtgacctt cgactctctc ccttcttccc
1261catcttcggc cacaccacac agccagaagc tagaccacct ccattggcca gtgttcagtg
1321acctcgataa catggcatcc agagaccatg atcatctaga caaccaccgg gagtctgaga
1381atagtgggga cagcggatac cccagtgaga agcggggtga gctggatgac cctgagcccc
1441gagaacatgg ccactcatac agtaatcgga agtacgagtc tgacgaagac agcctgggca
1501gctctggacg ggtatgtgta gagaagtgga atctcctcaa ctcctcccgc ctccacctgc
1561cgagggcttc ggccgtggcc ctggaagtgc aaaggcttaa tgctctggac ctcgaaaaga
1621aaatcgggaa gtccattttg gggaaggtcc atctggccat ggtgcgctac cacgagggtg
1681ggcgcttctg cgagaagggc gaggagtggg accaggagtc ggctgtcttc cacctggagc
1741acgcagccaa cctgggcgag ctggaggcca tcgtgggcct gggactcatg tactcgcagt
1801tgcctcatca catcctagcc gatgtctctc tgaaggagac agaagagaac aaaaccaaag
1861gatttgatta cttactaaag gccgctgaag ctggcgacag gcagtccatg atcctagtgg
1921cgcgagcttt tgactctggc cagaacctca gcccggacag gtgccaagac tggctagagg
1981ccctgcactg gtacaacact gccctggaga tgacggactg tgatgagggc ggtgagtacg
2041acggaatgca ggacgagccc cggtacatga tgctggccag ggaggccgag atgctgttca
2101caggaggcta cgggctggag aaggacccgc agagatcagg ggacttgtat acccaggcag
2161cagaggcagc gatggaagcc atgaagggcc gactggccaa ccagtactaC CAAAAGGCTG
2221AAGAGGCCTG GGCCCAGATG GAGGAGTAAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA
2281TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
2341CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACGCCG
2401GACCCGCAAT ACTGAATTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
2461tgaaagatt

Download FASTA (ORF) (Full)