Construct: ORF TRCN0000492338
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019571.2_s317c1
- DNA Barcode:
- GACACATACGAACAAAGCGCTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTHFR (4524)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492338
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | NM_005957.5 | 99.6% | 99.5% | (many diffs) |
| 2 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | XM_005263460.5 | 99.6% | 99.5% | (many diffs) |
| 3 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | XM_005263462.4 | 99.6% | 99.5% | (many diffs) |
| 4 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | XM_011541495.3 | 93.9% | 93.8% | (many diffs) |
| 5 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | NM_001330358.1 | 93.8% | 93.6% | (many diffs) |
| 6 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | XM_005263463.4 | 87.2% | 87% | (many diffs) |
| 7 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | XM_024447198.1 | 87.2% | 87% | (many diffs) |
| 8 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | XM_017001328.2 | 86.7% | 86.6% | (many diffs) |
| 9 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | XM_011541496.3 | 86.1% | 83.7% | (many diffs) |
| 10 | human | 4524 | MTHFR | methylenetetrahydrofolate r... | XR_002956640.1 | 59.3% | (many diffs) | |
| 11 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | NM_010840.3 | 86.8% | 90.1% | (many diffs) |
| 12 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | XM_006538619.1 | 86.5% | 89.7% | (many diffs) |
| 13 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | XM_006538620.3 | 86.5% | 89.7% | (many diffs) |
| 14 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | XM_006538621.1 | 86.5% | 89.7% | (many diffs) |
| 15 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | XM_006538622.3 | 86.5% | 89.7% | (many diffs) |
| 16 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | XM_006538618.3 | 82.2% | 85.3% | (many diffs) |
| 17 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | NM_001161798.1 | 81.7% | 84.8% | (many diffs) |
| 18 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | XM_006538617.3 | 81.4% | 84.4% | (many diffs) |
| 19 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | XR_390733.3 | 38.2% | (many diffs) | |
| 20 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | NR_027809.1 | 34.1% | (many diffs) | |
| 21 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | XR_390732.3 | 30.9% | (many diffs) | |
| 22 | mouse | 17769 | Mthfr | 5,10-methylenetetrahydrofol... | XR_390731.3 | 29.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2037
- ORF length:
- 1971
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatggt gaacgaagcc agaggaaaca gcagcctcaa cccctgcttg gagggcagtg 121 ccagcagtgg cagtgagagc tccaaagata gttcgagatg ttccaccccg ggcctggacc 181 ctgagcggca tgagagactc cgggagaaga tgaggcggcg attggaatct ggtgacaagt 241 ggttctccct ggaattcttc cctcctcgaa ctgctgaggg agctgtcaat ctcatctcaa 301 ggtttgaccg gatggcagca ggtggccccc tctacataga cgtgacctgg cacccagcag 361 gtgaccctgg ctcagacaag gagacctcct ccatgatgat cgccagcacc gccgtgaact 421 actgtggcct ggagaccatc ctgcacatga cctgctgccg tcagcgcctg gaggagatca 481 cgggccatct gcacaaagct aagcagctgg gcctgaagaa catcatggcg ctgcggggag 541 acccaatagg tgaccagtgg gaagaggagg agggaggctt caactacgca gtggacctgg 601 tgaagcacat ccgaagtgag tttggtgact actttgacat ctgtgtggca ggttacccca 661 aaggccaccc cgaagcaggg agctttgagg ctgacctgaa gcacttgaag gagaaggtgt 721 ctgcgggagc cgatttcatc atcacgcagc ttttctttga ggctgacaca ttcttccgct 781 ttgtgaaggc atgcaccgac atgggcatca cttgccccat cgtccccggg atctttccca 841 tccagggcta ccactccctt cggcagcttg tgaagctgtc caagctggag gtgccacagg 901 agatcaagga cgtgattgag ccaatcaaag acaacgatgc tgccatccgc aactatggca 961 tcgagctggc cgtgagcctg tgccaggagc ttctggccag tggcttggtg ccaggcctcc 1021 acttctacac cctcaaccgc gagatggcta ccacagaggt gctgaagcgc ctggggatgt 1081 ggactgagga ccccaggcgt cccctaccct gggctctcag tgcccacccc aagcgccgag 1141 aggaagatgt acgtcccatc ttctgggcct ccagaccaaa gagttacatc taccgtaccc 1201 aggagtggga cgagttccct aacggccgct ggggcaattc ctcttcccct gcctttgggg 1261 agctgaagga ctactacctc ttctacctga agagcaagtc ccccaaggag gagctgctga 1321 agatgtgggg ggaggagctg accagtgaag caagtgtctt tgaagtcttt gttctttacc 1381 tctcgggaga accaaaccgg aatggtcaca aagtgacttg cctgccctgg aacgatgagc 1441 ccctggcggc tgagaccagc ctgctgaagg aggagctgct gcgggtgaac cgccagggca 1501 tcctcaccat caactcacag cccaacatca acgggaagcc gtcctccgac cccatcgtgg 1561 gctggggccc cagcgggggc tatgtcttcc agaaggccta cttagagttt ttcacttccc 1621 gcgagacagc ggaagcacTT CTGCAAGTGC TGAAGAAGTA CGAGCTCCGG GTTAATTACC 1681 ACCTTGTCAA TGTGAAGGGT GAAAACATCA CCAATGCCCC TGAACTGCAG CCGAATGCTG 1741 TCACTTGGGG CATCTTCCCT GGGCGAGAGA TCATCCAGCC CACCGTAGTG GATCCCGTCA 1801 GCTTCATGTT CTGGAAGGAC GAGGCCTTTG CCCTGTGGAT TGAGCAGTGG GGAAAGCTGT 1861 ATGAGGAGGA GTCCCCGTCC CGCACCATCA TCCAGTACAT CCACGACAAC TACTTCCTGG 1921 TCAACCTGGT GGACAATGAC TTCCCACTGG ACAACTGCCT CTGGCAGGTG GTGGAAGACA 1981 CATTGGAGCT TCTCAACAGG CCCACCCAGA ATGCGAGAGA AACGGAGGCT CCAGACCCAG 2041 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2101 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2161 TATCTTGTGG AAAGGACGAG ACACATACGA ACAAAGCGCT TTCACGCGTT AAGTCgacaa 2221 tcaacctctg gattacaaaa tttgtgaaag att