Construct: ORF TRCN0000492347
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005586.2_s317c1
- Derived from:
- ccsbBroadEn_06789
- DNA Barcode:
- ACTATACCAGCGTTGCGTCAAACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSEN2 (5664)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492347
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5664 | PSEN2 | presenilin 2 | NM_000447.3 | 99.7% | 100% | 69T>C;129C>T;261C>T |
| 2 | human | 5664 | PSEN2 | presenilin 2 | XM_005273199.4 | 99.7% | 100% | 69T>C;129C>T;261C>T |
| 3 | human | 5664 | PSEN2 | presenilin 2 | XM_017001835.1 | 99.7% | 100% | 69T>C;129C>T;261C>T |
| 4 | human | 5664 | PSEN2 | presenilin 2 | NM_012486.2 | 99.5% | 99.7% | (many diffs) |
| 5 | human | 5664 | PSEN2 | presenilin 2 | XM_017001836.1 | 99.5% | 99.7% | (many diffs) |
| 6 | human | 5664 | PSEN2 | presenilin 2 | XR_001737317.2 | 64.3% | (many diffs) | |
| 7 | human | 5664 | PSEN2 | presenilin 2 | XR_001737321.1 | 55.3% | (many diffs) | |
| 8 | human | 5664 | PSEN2 | presenilin 2 | XR_001737318.2 | 51.8% | (many diffs) | |
| 9 | human | 5664 | PSEN2 | presenilin 2 | XR_949149.2 | 51.7% | (many diffs) | |
| 10 | human | 5664 | PSEN2 | presenilin 2 | XR_949150.3 | 47.8% | (many diffs) | |
| 11 | human | 5664 | PSEN2 | presenilin 2 | XR_001737319.1 | 45.7% | (many diffs) | |
| 12 | human | 5664 | PSEN2 | presenilin 2 | XR_001737320.1 | 45.6% | (many diffs) | |
| 13 | human | 5664 | PSEN2 | presenilin 2 | XR_001737316.2 | 34.1% | (many diffs) | |
| 14 | mouse | 19165 | Psen2 | presenilin 2 | NM_001128605.1 | 89.7% | 95.9% | (many diffs) |
| 15 | mouse | 19165 | Psen2 | presenilin 2 | NM_011183.3 | 89.7% | 95.9% | (many diffs) |
| 16 | mouse | 19165 | Psen2 | presenilin 2 | XM_011238776.2 | 89.7% | 95.9% | (many diffs) |
| 17 | mouse | 19165 | Psen2 | presenilin 2 | XM_006496712.2 | 89.5% | 95.7% | (many diffs) |
| 18 | mouse | 19165 | Psen2 | presenilin 2 | XR_373599.3 | 77% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1410
- ORF length:
- 1344
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct cacattcatg gcctctgaca gcgaggaaga agtgtgtgat gagcggacgt 121 ccctaatgtc ggccgagagc cccacgccgc gctcctgcca ggagggcagg cagggcccag 181 aggatggaga gaatactgcc cagtggagaa gccaggagaa cgaggaggac ggtgaggagg 241 accctgaccg ctatgtctgt agtggggttc ccgggcggcc gccaggcctg gaggaagagc 301 tgaccctcaa atacggagcg aagcatgtga tcatgctgtt tgtgcctgtc actctgtgca 361 tgatcgtggt ggtagccacc atcaagtctg tgcgcttcta cacagagaag aatggacagc 421 tcatctacac gccattcact gaggacacac cctcggtggg ccagcgcctc ctcaactccg 481 tgctgaacac cctcatcatg atcagcgtca tcgtggttat gaccatcttc ttggtggtgc 541 tctacaagta ccgctgctac aagttcatcc atggctggtt gatcatgtct tcactgatgc 601 tgctgttcct cttcacctat atctaccttg gggaagtgct caagacctac aatgtggcca 661 tggactaccc caccctcttg ctgactgtct ggaacttcgg ggcagtgggc atggtgtgca 721 tccactggaa gggccctctg gtgctgcagc aggcctacct catcatgatc agtgcgctca 781 tggccctagt gttcatcaag tacctcccag agtggtccgc gtgggtcatc ctgggcgcca 841 tctctgtgta tgatctcgtg gctgtgctgt gtcccaaagg gcctctgaga atgctggtag 901 aaactgccca ggagagaaat gagcccatat tccctgccct gatatactca tctgccatgg 961 tgtggacggt tggcatggcg aagctggacc cctcctctca gggtgccctc cagctccccT 1021 ACGACCCGGA GATGGAAGAA GACTCCTATG ACAGTTTTGG GGAGCCTTCA TACCCCGAAG 1081 TCTTTGAGCC TCCCTTGACT GGCTACCCAG GGGAGGAGCT GGAGGAAGAG GAGGAAAGGG 1141 GCGTGAAGCT TGGCCTCGGG GACTTCATCT TCTACAGTGT GCTGGTGGGC AAGGCGGCTG 1201 CCACGGGCAG CGGGGACTGG AATACCACGC TGGCCTGCTT CGTGGCCATC CTCATTGGCT 1261 TGTGTCTGAC CCTCCTGCTG CTTGCTGTGT TCAAGAAGGC GCTGCCCGCC CTCCCCATCT 1321 CCATCACGTT CGGGCTCATC TTTTACTTCT CCACGGACAA CCTGGTGCGG CCGTTCATGG 1381 ACACCCTGGC CTCCCATCAG CTCTACATCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1441 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1501 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAACTAT 1561 ACCAGCGTTG CGTCAAACCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1621 tgaaagatt