Construct: ORF TRCN0000492349
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006600.1_s317c1
- Derived from:
- ccsbBroadEn_14027
- DNA Barcode:
- GGGCCCACGGTCCAATCTCCTTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JADE3 (9767)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492349
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9767 | JADE3 | jade family PHD finger 3 | NM_001077445.2 | 99.9% | 99.1% | 2449_2450insC |
| 2 | human | 9767 | JADE3 | jade family PHD finger 3 | NM_014735.5 | 99.9% | 99.1% | 2449_2450insC |
| 3 | mouse | 382207 | Jade3 | jade family PHD finger 3 | NM_199317.2 | 84.6% | 81% | (many diffs) |
| 4 | mouse | 382207 | Jade3 | jade family PHD finger 3 | XM_011247476.1 | 82% | 78.6% | (many diffs) |
| 5 | mouse | 382207 | Jade3 | jade family PHD finger 3 | XM_011247477.1 | 82% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2541
- ORF length:
- 2472
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaacgccat aggcctgtca gcagcagtga cagttcagac gaaagtcctt 121 ccacttcctt tacttctggc tcaatgtata ggatcaagtc aaaaattcca aatgaacaca 181 agaaacctgc tgaggtattc cggaaggacc tcatcagtgc catgaaactt ccagattctc 241 accacattaa tcctgatagc tattacctct ttgctgatac atggaaggaa gaatgggaaa 301 agggagtcca ggtaccagcc agtccagaca ccgttccaca gccttctctc aggattatag 361 ctgagaaggt aaaggacgtt ctgtttatcc gaccccggaa gtatattcac tgctccagcc 421 cagacaccac agagcctggc tacatcaaca tcatggagtt ggcagcatct gtttgccgct 481 atgacctaga tgacatggac atcttctggc ttcaggaact caatgaagac cttgcagaaa 541 tgggttgtgg gccagttgat gagaatctta tggaaaagac agtagaagtc ctggaacgcc 601 attgccatga aaatatgaac catgctattg agacagagga agggctaggc atagagtatg 661 atgaagatgt gatctgtgat gtgtgccggt ctccagacag tgaagaaggg aatgatatgg 721 tgttctgtga taagtgtaac gtctgtgtgc atcaggcctg ctatggcatc ctcaaggtcc 781 cagaaggcag ctggctgtgt cgctcctgtg tcctgggcat ttatccgcaa tgtgtgttat 841 gtccaaagaa aggtggagcc ctgaagacca ccaagacagg gactaaatgg gctcatgtca 901 gctgtgccct gtggatccca gaggtcagca ttgcttgtcc tgagaggatg gaaccgatca 961 cgaagatctc ccacatccca cccagtcggt gggccttagt ctgcaacttg tgcaagttga 1021 agacgggggc ttgtattcag tgctctataa aaagctgcat cactgccttc cacgtcacct 1081 gtgcctttga gcacggccta gagatgaaga ccatcctaga tgagggagac gaagtgaagt 1141 tcaagtcata ttgcctcaag catagccaaa acaggcagaa acttggagaa gctgagtacc 1201 cccaccacag ggctaaagag cagagccagg ccaaaagtga gaaaaccagc ctgcgggcac 1261 agaagcttcg ggagctggag gaggagttct attccttggt acgagtggaa gatgtggccg 1321 cagagctggg tatgcccacg ctagctgtgg actttatcta taactactgg aaactgaagc 1381 ggaaaagtaa cttcaataag ccattatttc ctccaaagga ggatgaagaa aatgggctgg 1441 tgcagccaaa agaggaaagc attcacactc gaatgagaat gtttatgcat ctacgccagg 1501 acctggagag ggtccgaaat ctgtgctata tgataagcag acgagagaag ctgaagctgt 1561 cacacaacaa aatacaggaa cagatcttcg gtttgcaagt ccagcttctt aaccaagaaa 1621 ttgatgcagg gcttcctttg acaaatgcac ttgaaaactc actgttttac ccaccaccaa 1681 gaattacctt gaagttaaaa atgcccaaat caaccccaga agaccacaga aacagctcca 1741 cagaaaccga tcagcagccc cactctcctg acagcagctc atctgttcac agtataagga 1801 acatgcaggt gcctcaggag tcactagaaa tgagaacaaa atcgtatccg agatacccac 1861 tagagagcaa gaataaccgt ttgctggcca gtctcagcca ttctaggagt gaagcaaagg 1921 agtccagtcc tgcttggaga accccgtcct cggagtgcta tcatgggcag tcactgggaa 1981 agcctctggt ccttcaggct gccctccatg gacagtcttc cattgggaat gggaaaagtc 2041 agcctaactc caagtttgcc aaatccaatg gcctggaggg cagctggtct gggaatgtca 2101 cccaaaaaga cagctcgagt gagatgttct gtgaccagga gcctgtgttc agcccccact 2161 tggtcagtca gggcagcttt agaaaatcca ctgtagaaca ctttagtagg TCCTTTAAAG 2221 AGACCACCAA TAGGTGGGTG AAGAACACAG AGGACCTCCA GTGCTATGTG AAGCCAACCA 2281 AGAATATGAG CCCCAAGGAG CAGTTCTGGG GTAGACAGGT TCTCAGGCGG TCTGCAGGGA 2341 GAGCTCCATA TCAGGAAAAT GATGGCTATT GCCCAGATTT GGAGCTGAGT GATTCAGAGG 2401 CAGAAAGTGA TGGGAATAAA GAAAAAGTCA GGGTAAGGAA AGATAGCTCA GACAGGGAAA 2461 ATCCTCCCCA TGACTCTAGA CGGGATTGCC ATGGTAAAAG CAAGACACAT CCCCTTTCCC 2521 CACAGTTCAA TGCAAAGGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT 2581 ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA 2641 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGGCCC ACGGTCCAAT 2701 CTCCTTTGAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt