Transcript: Human NM_001077445.2

Homo sapiens jade family PHD finger 3 (JADE3), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
JADE3 (9767)
Length:
4713
CDS:
77..2548

Additional Resources:

NCBI RefSeq record:
NM_001077445.2
NBCI Gene record:
JADE3 (9767)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001077445.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000359549 CATTAATCCTGATAGCTATTA pLKO_005 253 CDS 100% 13.200 18.480 N JADE3 n/a
2 TRCN0000019557 GCCAGTTGATGAGAATCTTAT pLKO.1 559 CDS 100% 13.200 10.560 N JADE3 n/a
3 TRCN0000019556 GCAGAAAGTGATGGGAATAAA pLKO.1 2408 CDS 100% 15.000 10.500 N JADE3 n/a
4 TRCN0000359621 GGGCAGCTTTCCGGTTATATA pLKO_005 2817 3UTR 100% 15.000 10.500 N JADE3 n/a
5 TRCN0000019555 CCACCAAGACAGGGACTAAAT pLKO.1 876 CDS 100% 13.200 9.240 N JADE3 n/a
6 TRCN0000019558 CCCACTAGAGAGCAAGAATAA pLKO.1 1864 CDS 100% 13.200 9.240 N JADE3 n/a
7 TRCN0000019554 GCTCATCTGTTCACAGTATAA pLKO.1 1785 CDS 100% 13.200 9.240 N JADE3 n/a
8 TRCN0000359550 TTGTATTCAGTGCTCTATAAA pLKO_005 1039 CDS 100% 15.000 9.000 N JADE3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001077445.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14027 pDONR223 100% 99.9% 99.1% None 2449_2450insC n/a
2 ccsbBroad304_14027 pLX_304 0% 99.9% 99.1% V5 (not translated due to frame shift) 2449_2450insC n/a
3 TRCN0000492349 GGGCCCACGGTCCAATCTCCTTTG pLX_317 13.5% 99.9% 99.1% V5 (not translated due to prior stop codon) 2449_2450insC n/a
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