Construct: ORF TRCN0000492358
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002204.1_s317c1
- Derived from:
- ccsbBroadEn_06800
- DNA Barcode:
- TAGACCGAACCGTCATTCCATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMB7 (5695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492358
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5695 | PSMB7 | proteasome 20S subunit beta 7 | NM_002799.4 | 99.8% | 99.6% | 116T>C |
| 2 | mouse | 19177 | Psmb7 | proteasome (prosome, macrop... | NM_011187.1 | 90.1% | 96.3% | (many diffs) |
| 3 | mouse | 19177 | Psmb7 | proteasome (prosome, macrop... | XM_006497785.2 | 65.3% | 68.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 897
- ORF length:
- 831
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctgtgtcg gtgtatgctc caccagttgg aggcttctct tttgataact 121 gccgcaggaa tgccgtcttg gaagccgatt ttgcaaagag gggatacaag cttccaaagg 181 cccggaaaac tggcacgacc atcgctgggg tggtctataa ggatggcata gttcttggag 241 cagatacaag agcaactgaa gggatggttg ttgctgacaa gaactgttca aaaatacact 301 tcatatctcc taatatttat tgttgtggtg ctgggacagc tgcagacaca gacatgacaa 361 cccagctcat ttcttccaac ctggagctcc actccctctc cactggccgt cttcccagag 421 ttgtgacagc caaTCGGATG CTGAAGCAGA TGCTTTTCAG GTATCAAGGT TACATTGGTG 481 CAGCCCTAGT TTTAGGGGGA GTAGATGTTA CTGGACCTCA CCTCTACAGC ATCTATCCTC 541 ATGGATCAAC TGATAAGTTG CCTTATGTCA CCATGGGTTC TGGCTCCTTG GCAGCAATGG 601 CTGTATTTGA AGATAAGTTT AGGCCAGACA TGGAGGAGGA GGAAGCCAAG AATCTGGTGA 661 GCGAAGCCAT CGCAGCTGGC ATCTTCAACG ACCTGGGCTC CGGAAGCAAC ATTGACCTCT 721 GCGTCATCAG CAAGAACAAG CTGGATTTTC TCCGCCCATA CACAGTGCCC AACAAGAAGG 781 GGACCAGGCT TGGCCGGTAC AGGTGTGAGA AAGGGACTAC TGCAGTCCTC ACTGAGAAAA 841 TCACTCCTCT GGAGATTGAG GTGCTGGAAG AAACAGTCCA AACAATGGAC ACTTCCTGCC 901 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 961 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1021 ATATATCTTG TGGAAAGGAC GATAGACCGA ACCGTCATTC CATTTTACGC GTTAAGTCga 1081 caatcaacct ctggattaca aaatttgtga aagatt