Construct: ORF ccsbBroad304_00040

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010796.1_s304c1, BRDN0000399891
Derived from:
ccsbBroadEn_00040
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
APLNR (187)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00040
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 187 APLNR apelin receptor NM_005161.4 100% 100%
2 human 187 APLNR apelin receptor NR_027991.2 66% 1_246del;1387_1727del
3 mouse 23796 Aplnr apelin receptor NM_011784.3 86.9% 91.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1206
ORF length:
1140
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga ggaaggtggt gattttgaca actactatgg ggcagacaac cagtctgagt
121gtgagtacac agactggaaa tcctcggggg ccctcatccc tgccatctac atgttggtct
181tcctcctggg caccacgggc aacggtctgg tgctctggac cgtgtttcgg agcagccggg
241agaagaggcg ctcagctgat atcttcattg ctagcctggc ggtggctgac ctgaccttcg
301tggtgacgct gcccctgtgg gctacctaca cgtaccggga ctatgactgg ccctttggga
361ccttcttctg caagctcagc agctacctca tcttcgtcaa catgtacgcc agcgtcttct
421gcctcaccgg cctcagcttc gaccgctacc tggccatcgt gaggccagtg gccaatgctc
481ggctgaggct gcgggtcagc ggggccgtgg ccacggcagt tctttgggtg ctggccgccc
541tcctggccat gcctgtcatg gtgttacgca ccaccgggga cttggagaac accactaagg
601tgcagtgcta catggactac tccatggtgg ccactgtgag ctcagagtgg gcctgggagg
661tgggccttgg ggtctcgtcc accaccgtgg gctttgtggt gcccttcacc atcatgctga
721cctgttactt cttcatcgcc caaaccatcg ctggccactt ccgcaaggaa cgcatcgagg
781gcctgcggaa gcggcgccgg ctgctcagca tcatcgtggt gctggtggtg acctttgccc
841tgtgctggat gccctaccac ctggtgaaga cgctgtacat gctgggcagc ctgctgcact
901ggccctgtga ctttgacctc ttcctcatga acatcttccc ctactgcacc tgcatcagct
961acgtcaacag ctgcctcaac cccttcctct atgccttttt cgacccccgc ttccgccagg
1021cctgcacctc catgctctgc tgtggccaga gcaggtgcgc aggcacctcc cacagcagca
1081gtggggagaa gtcagccagc tactcttcgg ggcacagcca ggggcccggc cccaacatgg
1141gcaagggtgg agaacagatg cacgagaaat ccatccccta cagccaggag acccttgtgg
1201ttgactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1261gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1321acctctggat ta

Download FASTA (ORF) (Full)