Construct: ORF ccsbBroad304_00040
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010796.1_s304c1, BRDN0000399891
- Derived from:
- ccsbBroadEn_00040
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APLNR (187)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00040
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 187 | APLNR | apelin receptor | NM_005161.4 | 100% | 100% | |
| 2 | human | 187 | APLNR | apelin receptor | NR_027991.2 | 66% | 1_246del;1387_1727del | |
| 3 | mouse | 23796 | Aplnr | apelin receptor | NM_011784.3 | 86.9% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1206
- ORF length:
- 1140
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaaggtggt gattttgaca actactatgg ggcagacaac cagtctgagt 121 gtgagtacac agactggaaa tcctcggggg ccctcatccc tgccatctac atgttggtct 181 tcctcctggg caccacgggc aacggtctgg tgctctggac cgtgtttcgg agcagccggg 241 agaagaggcg ctcagctgat atcttcattg ctagcctggc ggtggctgac ctgaccttcg 301 tggtgacgct gcccctgtgg gctacctaca cgtaccggga ctatgactgg ccctttggga 361 ccttcttctg caagctcagc agctacctca tcttcgtcaa catgtacgcc agcgtcttct 421 gcctcaccgg cctcagcttc gaccgctacc tggccatcgt gaggccagtg gccaatgctc 481 ggctgaggct gcgggtcagc ggggccgtgg ccacggcagt tctttgggtg ctggccgccc 541 tcctggccat gcctgtcatg gtgttacgca ccaccgggga cttggagaac accactaagg 601 tgcagtgcta catggactac tccatggtgg ccactgtgag ctcagagtgg gcctgggagg 661 tgggccttgg ggtctcgtcc accaccgtgg gctttgtggt gcccttcacc atcatgctga 721 cctgttactt cttcatcgcc caaaccatcg ctggccactt ccgcaaggaa cgcatcgagg 781 gcctgcggaa gcggcgccgg ctgctcagca tcatcgtggt gctggtggtg acctttgccc 841 tgtgctggat gccctaccac ctggtgaaga cgctgtacat gctgggcagc ctgctgcact 901 ggccctgtga ctttgacctc ttcctcatga acatcttccc ctactgcacc tgcatcagct 961 acgtcaacag ctgcctcaac cccttcctct atgccttttt cgacccccgc ttccgccagg 1021 cctgcacctc catgctctgc tgtggccaga gcaggtgcgc aggcacctcc cacagcagca 1081 gtggggagaa gtcagccagc tactcttcgg ggcacagcca ggggcccggc cccaacatgg 1141 gcaagggtgg agaacagatg cacgagaaat ccatccccta cagccaggag acccttgtgg 1201 ttgactaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1261 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1321 acctctggat ta