Transcript: Human NR_027991.2

Homo sapiens apelin receptor (APLNR), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
APLNR (187)
Length:
1727
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_027991.2
NBCI Gene record:
APLNR (187)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_027991.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000008099 CGCTCAGCTGATATCTTCATT pLKO.1 430 3UTR 100% 5.625 7.875 N APLNR n/a
2 TRCN0000008100 CTACCTCATCTTCGTCAACAT pLKO.1 564 3UTR 100% 4.950 3.465 N APLNR n/a
3 TRCN0000008098 GCTGACCTGTTACTTCTTCAT pLKO.1 897 3UTR 100% 4.950 3.465 N APLNR n/a
4 TRCN0000008101 TGGAGAACAGATGCACGAGAA pLKO.1 1329 3UTR 100% 4.050 2.835 N APLNR n/a
5 TRCN0000356635 GAGAACAGATGCACGAGAAAT pLKO_005 1331 3UTR 100% 13.200 7.920 N APLNR n/a
6 TRCN0000008097 GCTTCTAGAAGGGAAGAAATT pLKO.1 1515 3UTR 100% 13.200 7.920 N APLNR n/a
7 TRCN0000221264 CCTGCCATCTACATGTTGGTT pLKO.1 340 3UTR 100% 3.000 2.100 N Aplnr n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_027991.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00040 pDONR223 100% 66% None 1_246del;1387_1727del n/a
2 ccsbBroad304_00040 pLX_304 0% 66% V5 1_246del;1387_1727del n/a
3 TRCN0000467691 CGATTAGGCTTAGGGCTGCGAGTT pLX_317 9.5% 66% V5 1_246del;1387_1727del n/a
4 TRCN0000491419 TATCAGGTTCTGCCAGGCCGGAGC pLX_317 25.4% 66% V5 1_246del;1387_1727del n/a
5 TRCN0000487737 ATTGGGATACTGGTGTTTCGATTA pLX_317 23.7% 66% V5 (not translated due to prior stop codon) 1_246del;1387_1727del n/a
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