Construct: ORF ccsbBroad304_00057
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008478.1_s304c1, BRDN0000404402
- Derived from:
- ccsbBroadEn_00057
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALOX12B (242)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00057
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 242 | ALOX12B | arachidonate 12-lipoxygenas... | NM_001139.3 | 100% | 100% | |
| 2 | mouse | 11686 | Alox12b | arachidonate 12-lipoxygenas... | NM_009659.2 | 83.8% | 86% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2169
- ORF length:
- 2103
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cacctacaaa gtcagggtgg ccacaggcac cgacctcttg tcgggaacac 121 gggactccat ctcactgacc attgtgggga cacaaggaga gagccataag cagctgctga 181 accactttgg gagagacttt gcaactgggg cggtgggcca gtacaccgtg cagtgccctc 241 aggacctggg tgagctcatc atcatccgcc tgcacaaaga gcggtacgcc ttcttcccca 301 aggacccttg gtactgcaac tatgtgcaga tctgtgcccc caacggccgt atctaccact 361 tccccgccta ccagtggatg gatggctacg agaccctggc actccgggag gccacaggaa 421 agacaacagc agatgactcg ctccccgtcc tcctggagca cagaaaagag gagatcagag 481 ccaagcagga cttctaccac tggcgagtct ttcttcctgg cctgcccagc tatgtgcaca 541 ttcccagtta ccgccctccg gtgcggaggc atcgcaaccc caaccggcct gagtggaatg 601 gctatattcc gggattccca attctcatca actttaaggc caccaagttc ctgaacttaa 661 atctccgcta ctccttcctc aagacggcct ccttcttcgt ccgcctgggg cccatggcac 721 tggctttcaa agtccgcggc ctgttggact gcaaacattc gtggaagagg ctgaaggaca 781 ttaggaaaat tttccctggc aagaaatctg tcgtctccga gtacgtggcc gagcactggg 841 cagaggacac cttctttggg taccagtacc tcaacggcgt caaccccggc ctgatccgcc 901 gctgcacgcg gatcccagac aagttccccg tcacagacga catggtggct ccgttcctgg 961 gcgagggaac gtgcttgcaa gcggagctgg agaaggggaa catttacctg gccgactacc 1021 gcatcatgga gggcatcccc accgtggagc tcagcggccg gaagcagcac cactgcgccc 1081 ccctctgcct gctgcacttt ggacccgagg gcaagatgat gcccatcgcc atccagctca 1141 gccagacccc tgggccagat tgccccatct tcctgcccag tgattctgag tgggactggc 1201 tgctagccaa gacgtgggta cgctatgcgg agttctacag ccacgaggcc atcgcccacc 1261 tgctggagac acacctcatt gctgaggcct tctgcctggc cttgctgagg aacctgccca 1321 tgtgccaccc cctctacaag ctcctcatcc cccatacccg atacaccgtc cagatcaaca 1381 gcattggccg ggccgttctc ctcaatgagg gggggctctc tgccaagggc atgtccctgg 1441 gcgtggaagg ctttgctggg gtgatggtac gggctctgtc ggagctcacc tatgacagcc 1501 tctacctccc caatgacttt gtggagcgtg gggtccagga cctgcctgga tattactacc 1561 gcgatgacag cttggcggtg tggaatgcac tggagaagta tgtgacggag atcatcacct 1621 attattaccc gagtgacgca gccgtggagg gtgatccgga attgcagtct tgggtgcagg 1681 aaatatttaa agagtgcctc ctggggcggg agagctcagg cttccctagg tgcttgcgaa 1741 ccgtgcctga gctgatccga tatgtcacta tagtcatcta cacctgctct gccaagcacg 1801 ctgctgtcaa cacaggccag atggagttca ccgcctggat gcccaacttc ccagcgtcca 1861 tgcggaatcc accgattcag actaaggggc tgaccactct ggagaccttc atggacacgt 1921 tgccggatgt gaagaccacg tgcatcacgc tgctggtgct ctggaccctc agccgagagc 1981 ctgacgacag gcggcccctg ggacacttcc cggacattca cttcgtggag gaggccccgc 2041 ggaggagcat agaggcgttc cgccagcgcc tgaaccagat ctcacacgac atccgccagc 2101 gcaacaagtg ccttcccatc ccctactact acctggaccc ggtgctgatt gagaacagca 2161 tttctattta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 2221 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 2281 tcaacctctg gatta