Construct: ORF ccsbBroad304_00139
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002358.1_s304c1, BRDN0000408458
- Derived from:
- ccsbBroadEn_00139
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V1G2 (534)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00139
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 534 | ATP6V1G2 | ATPase H+ transporting V1 s... | NM_130463.4 | 100% | 100% | |
| 2 | human | 534 | ATP6V1G2 | ATPase H+ transporting V1 s... | NM_001204078.2 | 66.1% | 66.1% | 100_101ins120 |
| 3 | human | 534 | ATP6V1G2 | ATPase H+ transporting V1 s... | NM_138282.3 | 65.2% | 65.2% | 0_1ins123 |
| 4 | mouse | 66237 | Atp6v1g2 | ATPase, H+ transporting, ly... | NM_023179.3 | 89.8% | 94.9% | (many diffs) |
| 5 | mouse | 66237 | Atp6v1g2 | ATPase, H+ transporting, ly... | NM_001347351.1 | 58.1% | 61% | (many diffs) |
| 6 | mouse | 66237 | Atp6v1g2 | ATPase, H+ transporting, ly... | XM_006524795.2 | 58.1% | 61% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 423
- ORF length:
- 354
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccagtcag tcccaaggta tccagcagct tctgcaagct gagaagcggg 121 cagctgagaa ggtggcagat gccagaaaga ggaaggcccg gcgactgaag caggcaaagg 181 aggaggcaca gatggaggtg gagcaatacc gcagagagcg agagcacgaa ttccagagca 241 agcagcaggc ggccatgggc tcccagggga acctgtctgc tgaggtggag caggctacaa 301 ggcgccaggt gcagggcatg cagagctccc agcagagaaa ccgagagcgt gtcctggccc 361 agcttcttgg catggtctgc gacgtcaggc cccaggtcca ccccaactac cggatttctg 421 ccttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 481 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 541 tctggatta