Construct: ORF ccsbBroad304_00148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011664.1_s304c1, BRDN0000408440
- Derived from:
- ccsbBroadEn_00148
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BAGE (574)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00148
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 574 | BAGE | B melanoma antigen | NM_001187.1 | 100% | 100% | |
| 2 | human | 85316 | BAGE5 | BAGE family member 5 | NM_182484.2 | 97.6% | 97.6% | 6A>G;10G>A;54A>G |
| 3 | human | 85317 | BAGE4 | BAGE family member 4 | NM_181704.1 | 88.3% | 86% | (many diffs) |
| 4 | human | 85318 | BAGE3 | BAGE family member 3 | NM_182481.1 | 36.3% | 34.8% | (many diffs) |
| 5 | human | 85319 | BAGE2 | BAGE family member 2 | NM_182482.2 | 36.3% | 34.8% | (many diffs) |
| 6 | human | 643648 | LINC01189 | long intergenic non-protein... | NR_046203.2 | 9.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 198
- ORF length:
- 129
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggccaga gcggtttttc tggcattgtc tgcccagctg ctccaagcca 121 ggctgatgaa ggaggagtcc cctgtggtga gctggaggtt ggagcctgaa gacggcacag 181 ctctgtgctt catcttcttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 241 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 301 agtcgacaat caacctctgg atta