Construct: ORF ccsbBroad304_00173

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017723.1_s304c1, BRDN0000407992
Derived from:
ccsbBroadEn_00173
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BRCA1 (672)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00173
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 672 BRCA1 BRCA1 DNA repair associated NM_007299.4 100% 100%
2 human 672 BRCA1 BRCA1 DNA repair associated NM_007298.3 92% 87.6% 2021_2094del;2172_2277del
3 human 672 BRCA1 BRCA1 DNA repair associated NM_007294.4 37.5% 36% (many diffs)
4 human 672 BRCA1 BRCA1 DNA repair associated NM_007300.4 37.1% 35.6% (many diffs)
5 human 672 BRCA1 BRCA1 DNA repair associated NM_007297.4 34.9% 33.5% (many diffs)
6 human 672 BRCA1 BRCA1 DNA repair associated NR_027676.2 28.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2163
ORF length:
2097
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tttatctgct cttcgcgttg aagaagtaca aaatgtcatt aatgctatgc
121agaaaatctt agagtgtccc atctgtctgg agttgatcaa ggaacctgtc tccacaaagt
181gtgaccacat attttgcaaa ttttgcatgc tgaaacttct caaccagaag aaagggcctt
241cacagtgtcc tttatgtaag aatgatataa ccaaaaggag cctacaagaa agtacgagat
301ttagtcaact tgttgaagag ctattgaaaa tcatttgtgc ttttcagctt gacacaggtt
361tggagtatgc aaacagctat aattttgcaa aaaaggaaaa taactctcct gaacatctaa
421aagatgaagt ttctatcatc caaagtatgg gctacagaaa ccgtgccaaa agacttctac
481agagtgaacc cgaaaatcct tccttgcagg aaaccagtct cagtgtccaa ctctctaacc
541ttggaactgt gagaactctg aggacaaagc agcggataca acctcaaaag acgtctgtct
601acattgaatt gggatctgat tcttctgaag ataccgttaa taaggcaact tattgcagtg
661tgggagatca agaattgtta caaatcaccc ctcaaggaac cagggatgaa atcagtttgg
721attctgcaaa aaaggctgct tgtgaatttt ctgagacgga tgtaacaaat actgaacatc
781atcaacccag taataatgat ttgaacacca ctgagaagcg tgcagctgag aggcatccag
841aaaagtatca gggtgaagca gcatctgggt gtgagagtga aacaagcgtc tctgaagact
901gctcagggct atcctctcag agtgacattt taaccactca gcagagggat accatgcaac
961ataacctgat aaagctccag caggaaatgg ctgaactaga agctgtgtta gaacagcatg
1021ggagccagcc ttctaacagc tacccttcca tcataagtga ctcttctgcc cttgaggacc
1081tgcgaaatcc agaacaaagc acatcagaaa aagtattaac ttcacagaaa agtagtgaat
1141accctataag ccagaatcca gaaggccttt ctgctgacaa gtttgaggtg tctgcagata
1201gttctaccag taaaaataaa gaaccaggag tggaaaggtc atccccttct aaatgcccat
1261cattagatga taggtggtac atgcacagtt gctctgggag tcttcagaat agaaactacc
1321catctcaaga ggagctcatt aaggttgttg atgtggagga gcaacagctg gaagagtctg
1381ggccacacga tttgacggaa acatcttact tgccaaggca agatctagag ggaacccctt
1441acctggaatc tggaatcagc ctcttctctg atgaccctga atctgatcct tctgaagaca
1501gagccccaga gtcagctcgt gTTGGCAACA TACCATCTTC AACCTCTGCA TTGAAAGTTC
1561CCCAATTGAA AGTTGCAGAA TCTGCCCAGA GTCCAGCTGC TGCTCATACT ACTGATACTG
1621CTGGGTATAA TGCAATGGAA GAAAGTGTGA GCAGGGAGAA GCCAGAATTG ACAGCTTCAA
1681CAGAAAGGGT CAACAAAAGA ATGTCCATGG TGGTGTCTGG CCTGACCCCA GAAGAATTTA
1741TGCTCGTGTA CAAGTTTGCC AGAAAACACC ACATCACTTT AACTAATCTA ATTACTGAAG
1801AGACTACTCA TGTTGTTATG AAAACAGATG CTGAGTTTGT GTGTGAACGG ACACTGAAAT
1861ATTTTCTAGG AATTGCGGGA GGAAAATGGG TAGTTAGCTA TTTCTGGGTG ACCCAGTCTA
1921TTAAAGAAAG AAAAATGCTG AATGAGCATG ATTTTGAAGT CAGAGGAGAT GTGGTCAATG
1981GAAGAAACCA CCAAGGTCCA AAGCGAGCAA GAGAATCCCA GGACAGAAAG ATCTTCAGGG
2041GGCTAGAAAT CTGTTGCTAT GGGCCCTTCA CCAACATGCC CACAGGGTGT CCACCCAATT
2101GTGGTTGTGC AGCCAGATGC CTGGACAGAG GACAATGGCT TCCATGCAAT TGGGCAGATG
2161TGTGCCCAAC TTTCTTGTAC aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
2221tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
2281tctggatta

Download FASTA (ORF) (Full)