Transcript: Human NM_007294.4

Homo sapiens BRCA1 DNA repair associated (BRCA1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
BRCA1 (672)
Length:
7088
CDS:
114..5705

Additional Resources:

NCBI RefSeq record:
NM_007294.4
NBCI Gene record:
BRCA1 (672)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_007294.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000244988 GCTATGCAAGGGTCCCTTAAA pLKO_005 5858 3UTR 100% 13.200 18.480 N BRCA1 n/a
2 TRCN0000221590 CCCTTCTAAATGCCCATCATT pLKO.1 4604 CDS 100% 5.625 7.875 N BRCA1 n/a
3 TRCN0000010305 GAGAATCCTAGAGATACTGAA pLKO.1 1197 CDS 100% 4.950 6.930 N BRCA1 n/a
4 TRCN0000221591 GCCTACAAGAAAGTACGAGAT pLKO.1 328 CDS 100% 4.050 5.670 N BRCA1 n/a
5 TRCN0000009823 TATAAGACCTCTGGCATGAAT pLKO.1 6765 3UTR 100% 5.625 4.500 N BRCA1 n/a
6 TRCN0000244987 ACTGATACTGCTGGGTATAAT pLKO_005 4971 CDS 100% 15.000 10.500 N BRCA1 n/a
7 TRCN0000244985 CAATATGGAACTCGAATTAAA pLKO_005 1889 CDS 100% 15.000 10.500 N BRCA1 n/a
8 TRCN0000244984 GAGTATGCAAACAGCTATAAT pLKO_005 411 CDS 100% 15.000 10.500 N BRCA1 n/a
9 TRCN0000244986 TTGCAACCTGAGGTCTATAAA pLKO_005 3378 CDS 100% 15.000 10.500 N BRCA1 n/a
10 TRCN0000221587 CCCTAAGTTTACTTCTCTAAA pLKO.1 6089 3UTR 100% 13.200 9.240 N BRCA1 n/a
11 TRCN0000221588 GCCCACCTAATTGTACTGAAT pLKO.1 2008 CDS 100% 4.950 3.465 N BRCA1 n/a
12 TRCN0000009824 TATAGCTGTTGGAAGGACTAG pLKO.1 6954 3UTR 100% 4.050 2.835 N BRCA1 n/a
13 TRCN0000221589 CCCACCTAATTGTACTGAATT pLKO.1 2009 CDS 100% 0.000 0.000 N BRCA1 n/a
14 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 6299 3UTR 100% 4.950 2.475 Y ERAP2 n/a
15 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 6300 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007294.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10699 pDONR223 100% 70.5% 70.3% None (many diffs) n/a
2 ccsbBroad304_10699 pLX_304 0% 70.5% 70.3% V5 (many diffs) n/a
3 TRCN0000470790 CGAGAAGCCCGACGCTAGTATCCC pLX_317 10.9% 70.5% 70.3% V5 (many diffs) n/a
4 ccsbBroadEn_00173 pDONR223 100% 37.5% 36% None (many diffs) n/a
5 ccsbBroad304_00173 pLX_304 30.7% 37.5% 36% V5 (many diffs) n/a
6 TRCN0000472103 TGTGCGGCCGCCACCACGTGCGCA pLX_317 23.8% 37.5% 36% V5 (many diffs) n/a
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