Transcript: Human NR_027676.2

Homo sapiens BRCA1 DNA repair associated (BRCA1), transcript variant 6, non-coding RNA.

Source:
NCBI, updated 2019-09-01
Taxon:
Homo sapiens (human)
Gene:
BRCA1 (672)
Length:
7152
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_027676.2
NBCI Gene record:
BRCA1 (672)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_027676.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000244988 GCTATGCAAGGGTCCCTTAAA pLKO_005 5922 3UTR 100% 13.200 18.480 N BRCA1 n/a
2 TRCN0000221590 CCCTTCTAAATGCCCATCATT pLKO.1 4668 3UTR 100% 5.625 7.875 N BRCA1 n/a
3 TRCN0000010305 GAGAATCCTAGAGATACTGAA pLKO.1 1261 3UTR 100% 4.950 6.930 N BRCA1 n/a
4 TRCN0000221591 GCCTACAAGAAAGTACGAGAT pLKO.1 395 3UTR 100% 4.050 5.670 N BRCA1 n/a
5 TRCN0000009823 TATAAGACCTCTGGCATGAAT pLKO.1 6829 3UTR 100% 5.625 4.500 N BRCA1 n/a
6 TRCN0000244987 ACTGATACTGCTGGGTATAAT pLKO_005 5035 3UTR 100% 15.000 10.500 N BRCA1 n/a
7 TRCN0000244985 CAATATGGAACTCGAATTAAA pLKO_005 1953 3UTR 100% 15.000 10.500 N BRCA1 n/a
8 TRCN0000244984 GAGTATGCAAACAGCTATAAT pLKO_005 478 3UTR 100% 15.000 10.500 N BRCA1 n/a
9 TRCN0000244986 TTGCAACCTGAGGTCTATAAA pLKO_005 3442 3UTR 100% 15.000 10.500 N BRCA1 n/a
10 TRCN0000221587 CCCTAAGTTTACTTCTCTAAA pLKO.1 6153 3UTR 100% 13.200 9.240 N BRCA1 n/a
11 TRCN0000221588 GCCCACCTAATTGTACTGAAT pLKO.1 2072 3UTR 100% 4.950 3.465 N BRCA1 n/a
12 TRCN0000009824 TATAGCTGTTGGAAGGACTAG pLKO.1 7018 3UTR 100% 4.050 2.835 N BRCA1 n/a
13 TRCN0000221589 CCCACCTAATTGTACTGAATT pLKO.1 2073 3UTR 100% 0.000 0.000 N BRCA1 n/a
14 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 6363 3UTR 100% 4.950 2.475 Y ERAP2 n/a
15 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 6364 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_027676.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10699 pDONR223 100% 55.2% None (many diffs) n/a
2 ccsbBroad304_10699 pLX_304 0% 55.2% V5 (many diffs) n/a
3 TRCN0000470790 CGAGAAGCCCGACGCTAGTATCCC pLX_317 10.9% 55.2% V5 (many diffs) n/a
4 ccsbBroadEn_00173 pDONR223 100% 28.8% None (many diffs) n/a
5 ccsbBroad304_00173 pLX_304 30.7% 28.8% V5 (many diffs) n/a
6 TRCN0000472103 TGTGCGGCCGCCACCACGTGCGCA pLX_317 23.8% 28.8% V5 (many diffs) n/a
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