Construct: ORF ccsbBroad304_00401

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002635.1_s304c1, BRDN0000410506
Derived from:
ccsbBroadEn_00401
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CYB5A (1528)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00401
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1528 CYB5A cytochrome b5 type A NM_148923.4 100% 100%
2 human 1528 CYB5A cytochrome b5 type A NM_001190807.3 92.5% 92.5% 258_259ins30
3 human 1528 CYB5A cytochrome b5 type A NM_001914.4 72.8% 72.3% 292C>A;294_295ins108
4 human 1528 CYB5A cytochrome b5 type A XM_011525835.2 72.8% 72.3% 292C>A;294_295ins108
5 mouse 109672 Cyb5a cytochrome b5 type A (micro... NM_025797.4 86.3% 87.3% (many diffs)
6 mouse 109672 Cyb5a cytochrome b5 type A (micro... NM_001348159.1 63.1% 63.4% (many diffs)
7 mouse 109672 Cyb5a cytochrome b5 type A (micro... XM_017317803.1 63.1% 63.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
468
ORF length:
402
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agagcagtcg gacgaggccg tgaagtacta caccctagag gagattcaga
121agcacaacca cagcaagagc acctggctga tcctgcacca caaggtgtac gatttgacca
181aatttctgga agagcatcct ggtggggaag aagttttaag ggaacaagct ggaggtgacg
241ctactgagaa ctttgaggat gtcgggcact ctacagatgc cagggaaatg tccaaaacat
301tcatcattgg ggagctccat ccagatgaca gaccaaagtt aaacaagcct ccggaaactc
361ttatcactac tattgattct agttccagtt ggtggaccaa ctgggtgatc cctgccatct
421ctgcagtggc cgtcgccttg atgtatcgcc tatacatggc agaggactgc ccaactttct
481tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
541aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)