Construct: ORF ccsbBroad304_00470
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003957.1_s304c1, BRDN0000400310
- Derived from:
- ccsbBroadEn_00470
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DVL2 (1856)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00470
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1856 | DVL2 | dishevelled segment polarit... | NM_004422.3 | 76.9% | 53.6% | (many diffs) |
| 2 | human | 1856 | DVL2 | dishevelled segment polarit... | XM_005256502.2 | 76.5% | 52.9% | (many diffs) |
| 3 | human | 1856 | DVL2 | dishevelled segment polarit... | XR_001752443.2 | 62.6% | (many diffs) | |
| 4 | human | 1856 | DVL2 | dishevelled segment polarit... | XR_001752442.2 | 58.5% | (many diffs) | |
| 5 | human | 1856 | DVL2 | dishevelled segment polarit... | XR_001752441.2 | 57.8% | (many diffs) | |
| 6 | mouse | 13543 | Dvl2 | dishevelled segment polarit... | NM_007888.3 | 68.8% | 51.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1389
- ORF length:
- 1323
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gggtagcagc actgggggcg gtggggttgg ggagacgaag gtgatttacc 121 acctggatga ggaagagact ccctacctgg tgaagatccc tgtccccgcc gagcgcatca 181 ccctcggcga tttcaagagc gtcctgcagc ggcccgcggg cgccaagtac tttttcaagt 241 ctatggatca ggatttcggg gtggtgaagg aagaaatttc agatgacaac gcccgcctcc 301 cctgcttcaa cggaagggtg gtatcctggc tggtgtcctc agataatccc caacccgaga 361 tggcccctcc agtccatgag cctcgggcag aactggcgcc tccagcccca cctttacctc 421 ctttgccacc cgagaggacc agcggcattg gggactcaag gcctccatcc ttccacccta 481 atgtgtccag cagccatgag aatctggagc ctgagacaga aaccgagtca gtagtgtcac 541 tgaggcggga gcggcctcgc aggagagaca gcagtgagca tggcgctggg ggccacagga 601 ctggtggccc ctcaaggctg gagcgccacc tggccggata cgagagctcc tctaccctca 661 tgaccagcga gctggagagt accagcctgg gggactcgga cgaggaggac accatgagca 721 ggttcagcag ctccacggag cagagcagtg cctcccgcct ccttaagcgc caccggcggc 781 gaaggaagca gaggccaccc cgcctggaga ggacgtcatc cttcagcagc gtcacagatt 841 ccacaatgtc tctcaatatc atcacagtca cgctaaacat ggagaagtac aacttcctgg 901 gtatctccat tgttggccag agcaatgagc ggggagacgg aggcatctac attggctcca 961 tcatgaaggg tggggctgtg gcggccgacg ggcgcattga gccaggggac atgcttttgc 1021 aggtgaatga catgaacttt gagaacatga gcaacgatga cgctgtgcgg gtgctgaggg 1081 acattgtgca caagcctggc cccattgtgc tgactgtggc caagtgctgg gatccctctc 1141 ctcaggccta tttcactctc ccccgaaatg agcccatcca gccaattgac cctgctgcct 1201 gggtgtccca ttccgcggct ctgactggca ccttcccagc ctatccaggt tcctcctccG 1261 TGAGCCCCAG TATTCCAGCT CAACTTCGCG CGATTCTGTG TACGTTCCCT AGGCGTGCAG 1321 AAACCTCTTT CCCTTTATTC GGGGAATGCT CAGAAGGCGA GAGCGCCGTC GTCTTTTACA 1381 GAAATAAATA AAATGGCTTA ACCATTAGGC TGGGAATGGC TGGAACTGGG CTAAGTTAAG 1441 GGCGGGATGG ATCAGAGTTA GGGCCGGGAT AGTTAGAACC ACACATGATA AGATACATTG 1501 ATGAGTTAGC ACAAACCACA ACTAGAATGC AGTGAAAAAA TTGCTTTATT TTGAAATTTG 1561 TGATGCTATT GCTTTATGTG TAACCATTAT AAGCTGCAAT AAACAAGTTC CTCTCACTCT 1621 CTGATATTCA TTTCTTTGCA AGTTATAAAT AGTGAATAAT AAGATGACAG GAACTACTAT 1681 ACGTACTGCT AGAGATTTTC CACACTGACT AAAAGGGTGT GAGGGATCTC TAGTTACCAG 1741 AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGATT TAGTGAGGCT 1801 TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG TCTAACCAGA 1861 GAGACCCGAT TATGTATCGA GCTAGGCACT TAAATACAAT ATCTATGCAA TGCGGCAATT 1921 CAGTGGTTCG TCCAATCCAT GTCAGACCCG TCTGTTGCCT TCCTAATAAG GCACGATCGT 1981 ACCACCTTAC TTCCACCAAT CGGCATGCAC GGTGCTTTTT CTCTCCGTGT AAGGCATGTT 2041 GCTAACTCAT CGTTACCATG ATGCAAGACG ACAAGAGTAT GGCATAAGTC TACATTAAGC 2101 TTGCAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTAATGC GCGCTGGGTA ATACGGCGGa 2161 gtgggtcgac acggagtgat gggggggcag ggcgcacggg gaggcccgag gagcgggccc 2221 ccgagtccaa gtccggcagt ggcagtgagt ctgagccctc cagccgaggg ggcagccttc 2281 ggcggggtgg ggaagcaagt gggactagcg atgggggccc tcctccatcc agaggctcaa 2341 ctgggggtgc ccctaatctc cgagcccacc cagggctcca tccctatgga ccgccccctg 2401 gcatggccct cccctacaac cccatgatgg tggtcatgat gcccccacct ccacctccag 2461 tccctccagc agtgcagcct ccgggggccc ctccagtcag agacctgggc tctgtgcccc 2521 cagaactgac agccagccgc caaagcttcc acatggccat gggcaatccc agcgagttct 2581 ttgtggatgt tatgtaccca actttcttgt acaaagtggt tggtaagcct atccctaacc 2641 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2701 cgacaatcaa cctctggatt a