Construct: ORF ccsbBroad304_00470

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003957.1_s304c1, BRDN0000400310
Derived from:
ccsbBroadEn_00470
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DVL2 (1856)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00470
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1856 DVL2 dishevelled segment polarit... NM_004422.3 76.9% 53.6% (many diffs)
2 human 1856 DVL2 dishevelled segment polarit... XM_005256502.2 76.5% 52.9% (many diffs)
3 human 1856 DVL2 dishevelled segment polarit... XR_001752443.2 62.6% (many diffs)
4 human 1856 DVL2 dishevelled segment polarit... XR_001752442.2 58.5% (many diffs)
5 human 1856 DVL2 dishevelled segment polarit... XR_001752441.2 57.8% (many diffs)
6 mouse 13543 Dvl2 dishevelled segment polarit... NM_007888.3 68.8% 51.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1389
ORF length:
1323
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gggtagcagc actgggggcg gtggggttgg ggagacgaag gtgatttacc
121acctggatga ggaagagact ccctacctgg tgaagatccc tgtccccgcc gagcgcatca
181ccctcggcga tttcaagagc gtcctgcagc ggcccgcggg cgccaagtac tttttcaagt
241ctatggatca ggatttcggg gtggtgaagg aagaaatttc agatgacaac gcccgcctcc
301cctgcttcaa cggaagggtg gtatcctggc tggtgtcctc agataatccc caacccgaga
361tggcccctcc agtccatgag cctcgggcag aactggcgcc tccagcccca cctttacctc
421ctttgccacc cgagaggacc agcggcattg gggactcaag gcctccatcc ttccacccta
481atgtgtccag cagccatgag aatctggagc ctgagacaga aaccgagtca gtagtgtcac
541tgaggcggga gcggcctcgc aggagagaca gcagtgagca tggcgctggg ggccacagga
601ctggtggccc ctcaaggctg gagcgccacc tggccggata cgagagctcc tctaccctca
661tgaccagcga gctggagagt accagcctgg gggactcgga cgaggaggac accatgagca
721ggttcagcag ctccacggag cagagcagtg cctcccgcct ccttaagcgc caccggcggc
781gaaggaagca gaggccaccc cgcctggaga ggacgtcatc cttcagcagc gtcacagatt
841ccacaatgtc tctcaatatc atcacagtca cgctaaacat ggagaagtac aacttcctgg
901gtatctccat tgttggccag agcaatgagc ggggagacgg aggcatctac attggctcca
961tcatgaaggg tggggctgtg gcggccgacg ggcgcattga gccaggggac atgcttttgc
1021aggtgaatga catgaacttt gagaacatga gcaacgatga cgctgtgcgg gtgctgaggg
1081acattgtgca caagcctggc cccattgtgc tgactgtggc caagtgctgg gatccctctc
1141ctcaggccta tttcactctc ccccgaaatg agcccatcca gccaattgac cctgctgcct
1201gggtgtccca ttccgcggct ctgactggca ccttcccagc ctatccaggt tcctcctccG
1261TGAGCCCCAG TATTCCAGCT CAACTTCGCG CGATTCTGTG TACGTTCCCT AGGCGTGCAG
1321AAACCTCTTT CCCTTTATTC GGGGAATGCT CAGAAGGCGA GAGCGCCGTC GTCTTTTACA
1381GAAATAAATA AAATGGCTTA ACCATTAGGC TGGGAATGGC TGGAACTGGG CTAAGTTAAG
1441GGCGGGATGG ATCAGAGTTA GGGCCGGGAT AGTTAGAACC ACACATGATA AGATACATTG
1501ATGAGTTAGC ACAAACCACA ACTAGAATGC AGTGAAAAAA TTGCTTTATT TTGAAATTTG
1561TGATGCTATT GCTTTATGTG TAACCATTAT AAGCTGCAAT AAACAAGTTC CTCTCACTCT
1621CTGATATTCA TTTCTTTGCA AGTTATAAAT AGTGAATAAT AAGATGACAG GAACTACTAT
1681ACGTACTGCT AGAGATTTTC CACACTGACT AAAAGGGTGT GAGGGATCTC TAGTTACCAG
1741AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA AGGCAAGATT TAGTGAGGCT
1801TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC TCAGATCTGG TCTAACCAGA
1861GAGACCCGAT TATGTATCGA GCTAGGCACT TAAATACAAT ATCTATGCAA TGCGGCAATT
1921CAGTGGTTCG TCCAATCCAT GTCAGACCCG TCTGTTGCCT TCCTAATAAG GCACGATCGT
1981ACCACCTTAC TTCCACCAAT CGGCATGCAC GGTGCTTTTT CTCTCCGTGT AAGGCATGTT
2041GCTAACTCAT CGTTACCATG ATGCAAGACG ACAAGAGTAT GGCATAAGTC TACATTAAGC
2101TTGCAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTAATGC GCGCTGGGTA ATACGGCGGa
2161gtgggtcgac acggagtgat gggggggcag ggcgcacggg gaggcccgag gagcgggccc
2221ccgagtccaa gtccggcagt ggcagtgagt ctgagccctc cagccgaggg ggcagccttc
2281ggcggggtgg ggaagcaagt gggactagcg atgggggccc tcctccatcc agaggctcaa
2341ctgggggtgc ccctaatctc cgagcccacc cagggctcca tccctatgga ccgccccctg
2401gcatggccct cccctacaac cccatgatgg tggtcatgat gcccccacct ccacctccag
2461tccctccagc agtgcagcct ccgggggccc ctccagtcag agacctgggc tctgtgcccc
2521cagaactgac agccagccgc caaagcttcc acatggccat gggcaatccc agcgagttct
2581ttgtggatgt tatgtaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
2641ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2701cgacaatcaa cctctggatt a

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.