Construct: ORF ccsbBroad304_00475

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002243.1_s304c1, BRDN0000405187
Derived from:
ccsbBroadEn_00475
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EDA (1896)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00475
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1896 EDA ectodysplasin A NM_001399.5 100% 100%
2 human 1896 EDA ectodysplasin A NM_001005609.2 99.4% 99.4% 918_919insGTAGAA
3 human 1896 EDA ectodysplasin A XM_006724630.2 99.2% 99.2% 792_793insGATCTTTCA
4 human 1896 EDA ectodysplasin A NM_001005612.3 98.7% 98.7% 792_793insGATCTTTCA;909_910insGTAGAA
5 human 1896 EDA ectodysplasin A XM_017029336.1 96.4% 96.4% 882_883ins42
6 human 1896 EDA ectodysplasin A XM_011530885.2 82.7% 80.1% (many diffs)
7 human 1896 EDA ectodysplasin A NM_001005613.4 35.9% 33.5% (many diffs)
8 human 1896 EDA ectodysplasin A NM_001005610.4 34.1% 33.7% (many diffs)
9 human 1896 EDA ectodysplasin A XM_017029337.1 34% 33.7% 397_398insTG;399_400ins772
10 mouse 13607 Eda ectodysplasin-A NM_010099.2 91.4% 94.6% (many diffs)
11 mouse 13607 Eda ectodysplasin-A NM_001177937.1 90.9% 94.1% (many diffs)
12 mouse 13607 Eda ectodysplasin-A NM_001177938.1 90.7% 93.8% (many diffs)
13 mouse 13607 Eda ectodysplasin-A NM_001177939.1 90.1% 93.3% (many diffs)
14 mouse 13607 Eda ectodysplasin-A NM_001177940.1 89.5% 92.5% (many diffs)
15 mouse 13607 Eda ectodysplasin-A NM_001177941.1 88.3% 91.3% (many diffs)
16 mouse 13607 Eda ectodysplasin-A NM_001177942.1 87.8% 91% (many diffs)
17 mouse 13607 Eda ectodysplasin-A NM_001177943.1 78% 71.5% (many diffs)
18 mouse 13607 Eda ectodysplasin-A NM_001177944.1 78% 71.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1242
ORF length:
1173
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggctacccg gaggtggagc gcagggaact cctgcctgca gcagcgccgc
121gggagcgagg gagccagggc tgcgggtgtg gcggggcccc tgcccgggcg ggcgaaggga
181acagctgcct gctcttcctg ggtttctttg gcctctcgct ggccctccac ctgctgacgt
241tgtgctgcta cctagagttg cgctcggagt tgcggcggga acgtggagcc gagtcccgcc
301ttggcggctc gggcacccct ggcacctctg gcaccctaag cagcctcggt ggcctcgacc
361ctgacagccc catcaccagt caccttgggc agccgtcacc taagcagcag ccattggaac
421cgggagaagc cgcactccac tctgactccc aggacgggca ccagatggcc ctattgaatt
481tcttcttccc tgatgaaaag ccatactctg aagaagaaag taggcgtgtt cgccgcaata
541aaagaagcaa aagcaatgaa ggagcagatg gcccagttaa aaacaagaaa aagggaaaga
601aagcaggacc tcctggaccc aatggccctc caggaccccc aggacctcca ggaccccagg
661gacccccagg aattccaggg attcctggaa ttccaggaac aactgttatg ggaccacctg
721gtcctccagg tcctcctggt cctcaaggac cccctggcct ccagggacct tctggtgctg
781ctgataaagc tggaactcga gaaaaccagc cagctgtggt gcatctacag ggccaagggt
841cagcaattca agtcaagaat gatctttcag gtggagtgct caatgactgg tctcgcatca
901ctatgaaccc caaggtgttt aagctacatc cccgcagcgg ggagctggag gtactggtgg
961acggcaccta cttcatctat agtcaggtag aagtatacta catcaacttc actgactttg
1021ccagctatga ggtggtggtg gatgagaagc ccttcctgca gtgcacacgc agcatcgaga
1081cgggcaagac caactacaac acttgctata ccgcaggcgt ctgcctcctc aaggcccggc
1141agaagatcgc cgtcaagatg gtgcacgctg acatctccat caacatgagc aagcacacca
1201cgttctttgg ggccatcagg ctgggtgaag cccctgcatc cttgccaact ttcttgtaca
1261aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1321ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)