Construct: ORF ccsbBroad304_00513
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012047.1_s304c1, BRDN0000403758
- Derived from:
- ccsbBroadEn_00513
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERCC3 (2071)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00513
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2071 | ERCC3 | ERCC excision repair 3, TFI... | NM_000122.2 | 100% | 100% | |
| 2 | human | 2071 | ERCC3 | ERCC excision repair 3, TFI... | XM_011510794.2 | 99.2% | 99.2% | 658_675del |
| 3 | human | 2071 | ERCC3 | ERCC excision repair 3, TFI... | NM_001303416.2 | 91.8% | 91.8% | 0_1ins192 |
| 4 | human | 2071 | ERCC3 | ERCC excision repair 3, TFI... | NM_001303418.2 | 91.8% | 91.8% | 0_1ins192 |
| 5 | human | 2071 | ERCC3 | ERCC excision repair 3, TFI... | XM_017003583.1 | 80.5% | 80.5% | 0_1ins456 |
| 6 | human | 2071 | ERCC3 | ERCC excision repair 3, TFI... | XM_011510795.1 | 79.9% | 79.9% | 0_1ins456;202_219del |
| 7 | mouse | 13872 | Ercc3 | excision repair cross-compl... | NM_133658.1 | 87.3% | 95.7% | (many diffs) |
| 8 | mouse | 13872 | Ercc3 | excision repair cross-compl... | XM_006525636.2 | 72.7% | 69.4% | (many diffs) |
| 9 | mouse | 13872 | Ercc3 | excision repair cross-compl... | XR_385973.1 | 72.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2412
- ORF length:
- 2346
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg caaaagagac cgagcggacc gcgacaagaa gaaatccagg aagcggcact 121 atgaggatga agaggatgat gaagaggacg ccccggggaa cgaccctcag gaagcggttc 181 cctcggcggc ggggaagcag gtggatgagt caggcaccaa agtggatgaa tatggagcca 241 aggactacag gctgcaaatg ccgctgaagg acgaccacac ctccaggccc ctctgggtgg 301 ctcccgatgg ccatatcttc ttggaagcct tctctccagt ttacaaatat gcccaagact 361 tcttggtggc tattgcagag ccagtgtgcc gaccaaccca tgtgcatgag tacaaactaa 421 ctgcctactc cttgtatgca gctgtcagcg ttgggctgca aaccagtgac atcaccgagt 481 acctcaggaa gctcagcaag actggagtcc ctgatggaat tatgcagttt attaagttgt 541 gtactgtcag ctatggaaaa gtcaagctgg tcttgaagca caacagatac ttcgttgaaa 601 gttgccaccc tgatgtaatc cagcatcttc tccaggaccc cgtgatccga gaatgccgct 661 taagaaactc tgaaggggag gccactgagc tcatcacaga gactttcaca agcaaatctg 721 ccatttctaa gactgctgaa agcagtggtg ggccctccac ttcccgagtg acagatccac 781 agggtaaatc tgacatcccc atggacctgt ttgacttcta tgagcaaatg gacaaggatg 841 aagaagaaga agaagagaca cagacagtgt cttttgaagt caagcaggaa atgattgagg 901 aactccagaa acgttgcatc cacctggagt accctctgtt ggcagaatat gacttccgga 961 atgattctgt caaccctgat atcaacattg acctaaagcc cacagctgtc ctcagaccct 1021 atcaggagaa gagcttgcga aagatgtttg gaaacgggcg tgcacgttcg ggggtcattg 1081 ttcttccctg cggtgctgga aagtccctgg ttggtgtgac tgctgcatgc actgtcagaa 1141 aacgctgtct ggtgctgggc aactcagctg tttctgtgga gcagtggaaa gcccagttca 1201 agatgtggtc caccattgac gacagccaga tctgccggtt cacctccgat gccaaggaca 1261 agcccatcgg ctgctccgtt gccattagca cctactccat gctgggccac accaccaaaa 1321 ggtcctggga ggccgagcga gtcatggagt ggctcaagac ccaggagtgg ggcctcatga 1381 tcctggatga agtgcacacc ataccagcca agatgttccg aagggtgctc accatcgtgc 1441 aggcccactg taagctgggt ttgactgcga ccctcgtccg cgaagatgac aaaattgtgg 1501 atttaaattt tctgattggg cctaagctct acgaagccaa ctggatggag ctgcagaata 1561 atggctacat cgccaaagtc cagtgtgctg aggtctggtg ccctatgtct cctgaatttt 1621 accgggaata tgtggcaatc aaaaccaaga aacgaatctt gctgtacacc atgaacccca 1681 acaaatttag agcttgccag tttctgatca agtttcatga aaggaggaat gacaagatta 1741 ttgtctttgc tgacaatgtg tttgccctaa aggaatatgc cattcgactg aacaaaccct 1801 atatctacgg acctacgtct cagggggaaa ggatgcaaat tctccagaat ttcaagcaca 1861 accccaaaat taacaccatc ttcatatcca aggtaggtga cacttcgttt gatctgccgg 1921 aagcaaatgt cctcattcag atctcatccc atggtggctc caggcgtcag gaagcccaaa 1981 ggctagggcg ggtgcttcga gctaaaaaag ggatggttgc agaagagtac aatgcctttt 2041 tctactcact ggtatcccag gacacacagg aaatggctta ctcaaccaag cggcagagat 2101 tcttggtaga tcaaggttat agcttcaagg tgatcacgaa actcgctggc atggaggagg 2161 aagacttggc gttttcgaca aaagaagagc aacagcagct cttacagaaa gtcctggcag 2221 ccactgacct ggatgccgag gaggaggtgg tggctgggga atttggctcc agatccagcc 2281 aggcatctcg gcgctttggc accatgagtt ctatgtctgg ggccgacgac actgtgtaca 2341 tggagtacca ctcatcgcgg agcaaggcgc ccagcaaaca tgtacacccg ctcttcaagc 2401 gctttaggaa atgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 2461 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 2521 caatcaacct ctggatta