Construct: ORF ccsbBroad304_00526

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015219.1_s304c1, BRDN0000400328
Derived from:
ccsbBroadEn_00526
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EZH2 (2146)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00526
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2146 EZH2 enhancer of zeste 2 polycom... NM_004456.5 100% 100%
2 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515884.2 100% 100%
3 human 2146 EZH2 enhancer of zeste 2 polycom... NM_001203247.2 99.3% 99.2% 892_893insGTAAGTGCAATTATT
4 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515883.2 98.9% 98.9% 1_24del
5 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011817.2 98.9% 98.9% 1_24del
6 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515887.3 98.8% 98.8% 219_220ins27
7 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515888.2 98.8% 98.8% 219_220ins27
8 human 2146 EZH2 enhancer of zeste 2 polycom... XM_005249962.4 98.2% 98.1% 1_24del;916_917insGTAAGTGCAATTATT
9 human 2146 EZH2 enhancer of zeste 2 polycom... NM_001203248.2 98.1% 98% 219_220ins27;865_866insGTAAGTGCAATTATT
10 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515885.2 97.7% 97.7% 1_24del;243_244ins27
11 human 2146 EZH2 enhancer of zeste 2 polycom... XM_005249963.4 97.1% 96.9% 1_24del;243_244ins27;889_890insGTAAGTGCAATTATT
12 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515894.2 94.8% 94.8% 245_246ins117
13 human 2146 EZH2 enhancer of zeste 2 polycom... NM_152998.3 94.1% 94% 245_246ins117;775_776insGTAAGTGCAATTATT
14 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515890.2 93.8% 93.8% 1_24del;269_270ins117
15 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011820.2 93.6% 93.6% 219_220ins144
16 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515892.2 93.4% 93.2% 1_24del;1570_1571ins126
17 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515893.2 93.1% 93% 1_24del;269_270ins117;799_800insGTAAGTGCAATTATT
18 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011819.1 92.7% 92.4% 1_24del;916_917insGTAAGTGCAATTATT;1555_1556ins126
19 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515895.2 92.6% 92.6% 1_24del;243_244ins144
20 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515891.3 92.6% 90.9% (many diffs)
21 human 2146 EZH2 enhancer of zeste 2 polycom... NM_001203249.2 92.5% 92.2% 219_220ins27;865_866insGTAAGTGCAATTATT;1504_1505ins126
22 human 2146 EZH2 enhancer of zeste 2 polycom... XM_024446680.1 91.9% 90.1% (many diffs)
23 human 2146 EZH2 enhancer of zeste 2 polycom... XM_005249964.4 91.5% 91.3% (many diffs)
24 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515896.2 87.6% 87.3% (many diffs)
25 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515889.2 87.6% 86.6% (many diffs)
26 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515886.2 86% 87.2% (many diffs)
27 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515897.2 85.4% 85.4% 0_1ins327
28 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515898.2 85.4% 85.4% 0_1ins327
29 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011818.1 85.4% 86.4% (many diffs)
30 human 2146 EZH2 enhancer of zeste 2 polycom... XM_017011821.1 84.8% 84.6% 0_1ins327;565_566insGTAAGTGCAATTATT
31 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515899.3 66.1% 66.2% 1_24del;1531delC;1533_1534ins745
32 human 2146 EZH2 enhancer of zeste 2 polycom... XM_011515901.3 55.3% 54.1% (many diffs)
33 human 2146 EZH2 enhancer of zeste 2 polycom... XR_002956413.1 40.1% (many diffs)
34 human 2146 EZH2 enhancer of zeste 2 polycom... XR_001744581.1 38.5% 1_2639del;3878_3879ins265;4628_4893del
35 human 2146 EZH2 enhancer of zeste 2 polycom... XR_002956414.1 37.4% (many diffs)
36 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505520.3 92.5% 98.2% (many diffs)
37 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505521.2 92.5% 98.2% (many diffs)
38 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505526.3 92.5% 98.2% (many diffs)
39 mouse 14056 Ezh2 enhancer of zeste 2 polycom... NM_007971.2 91.9% 97.6% (many diffs)
40 mouse 14056 Ezh2 enhancer of zeste 2 polycom... NM_001146689.1 91.3% 97% (many diffs)
41 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505523.1 90.7% 96.4% (many diffs)
42 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505524.1 86.9% 92.5% (many diffs)
43 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505525.1 85.1% 90.6% (many diffs)
44 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505527.1 57.9% 54.3% (many diffs)
45 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505528.1 57.9% 54.3% (many diffs)
46 mouse 14056 Ezh2 enhancer of zeste 2 polycom... XM_006505529.1 57.3% 54.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2319
ORF length:
2253
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg ccagactggg aagaaatctg agaagggacc agtttgttgg cggaagcgtg
121taaaatcaga gtacatgcga ctgagacagc tcaagaggtt cagacgagct gatgaagtaa
181agagtatgtt tagttccaat cgtcagaaaa ttttggaaag aacggaaatc ttaaaccaag
241aatggaaaca gcgaaggata cagcctgtgc acatcctgac ttctgtgagc tcattgcgcg
301ggactaggga gtgttcggtg accagtgact tggattttcc aacacaagtc atcccattaa
361agactctgaa tgcagttgct tcagtaccca taatgtattc ttggtctccc ctacagcaga
421attttatggt ggaagatgaa actgttttac ataacattcc ttatatggga gatgaagttt
481tagatcagga tggtactttc attgaagaac taataaaaaa ttatgatggg aaagtacacg
541gggatagaga atgtgggttt ataaatgatg aaatttttgt ggagttggtg aatgcccttg
601gtcaatataa tgatgatgac gatgatgatg atggagacga tcctgaagaa agagaagaaa
661agcagaaaga tctggaggat caccgagatg ataaagaaag ccgcccacct cggaaatttc
721cttctgataa aatttttgaa gccatttcct caatgtttcc agataagggc acagcagaag
781aactaaagga aaaatataaa gaactcaccg aacagcagct cccaggcgca cttcctcctg
841aatgtacccc caacatagat ggaccaaatg ctaaatctgt tcagagagag caaagcttac
901actcctttca tacgcttttc tgtaggcgat gttttaaata tgactgcttc ctacatcgta
961agtgcaatta ttcttttcat gcaacaccca acacttataa gcggaagaac acagaaacag
1021ctctagacaa caaaccttgt ggaccacagt gttaccagca tttggaggga gcaaaggagt
1081ttgctgctgc tctcaccgct gagcggataa agaccccacc aaaacgtcca ggaggccgca
1141gaagaggacg gcttcccaat aacagtagca ggcccagcac ccccaccatt aatgtgctgg
1201aatcaaagga tacagacagt gatagggaag cagggactga aacgggggga gagaacaatg
1261ataaagaaga agaagagaag aaagatgaaa cttcgagctc ctctgaagca aattctcggt
1321gtcaaacacc aataaagatg aagccaaata ttgaacctcc tgagaatgtg gagtggagtg
1381gtgctgaagc ctcaatgttt agagtcctca ttggcactta ctatgacaat ttctgtgcca
1441ttgctaggtt aattgggacc aaaacatgta gacaggtgta tgagtttaga gtcaaagaat
1501ctagcatcat agctccagct cccgctgagg atgtggatac tcctccaagg aaaaagaaga
1561ggaaacaccg gttgtgggct gcacactgca gaaagataca gctgaaaaag gacggctcct
1621ctaaccatgt ttacaactat caaccctgtg atcatccacg gcagccttgt gacagttcgt
1681gcccttgtgt gatagcacaa aatttttgtg aaaagttttg tcaatgtagt tcagagtgtc
1741aaaaccgctt tccgggatgc cgctgcaaag cacagtgcaa caccaagcag tgcccgtgct
1801acctggctgt ccgagagtgt gaccctgacc tctgtcttac ttgtggagcc gctgaccatt
1861gggacagtaa aaatgtgtcc tgcaagaact gcagtattca gcggggctcc aaaaagcatc
1921tattgctggc accatctgac gtggcaggct gggggatttt tatcaaagat cctgtgcaga
1981aaaatgaatt catctcagaa tactgtggag agattatttc tcaagatgaa gctgacagaa
2041gagggaaagt gtatgataaa tacatgtgca gctttctgtt caacttgaac aatgattttg
2101tggtggatgc aacccgcaag ggtaacaaaa ttcgttttgc aaatcattcg gtaaatccaa
2161actgctatgc aaaagttatg atggttaacg gtgatcacag gataggtatt tttgccaaga
2221gagccatcca gactggcgaa gagctgtttt ttgattacag atacagccag gctgatgccc
2281tgaagtatgt cggcatcgaa agagaaatgg aaatcccttg cccaactttc ttgtacaaag
2341tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
2401gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.