Construct: ORF ccsbBroad304_00531
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002593.1_s304c1, BRDN0000401584
- Derived from:
- ccsbBroadEn_00531
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- F9 (2158)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00531
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2158 | F9 | coagulation factor IX | NM_000133.4 | 100% | 100% | |
| 2 | human | 2158 | F9 | coagulation factor IX | NM_001313913.1 | 91.7% | 91.7% | 276_277ins114 |
| 3 | human | 2158 | F9 | coagulation factor IX | XM_005262397.4 | 90.6% | 90.6% | 389_390ins129 |
| 4 | mouse | 14071 | F9 | coagulation factor IX | NM_007979.2 | 84.3% | 80.2% | (many diffs) |
| 5 | mouse | 14071 | F9 | coagulation factor IX | NM_001305797.1 | 77.3% | 73.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1452
- ORF length:
- 1383
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcacnat gcagcgcgtg aacatgatca tggcagaatc accaggcctc atcaccatct 121 gccttttagg atatctactc agtgctgaat gtacagtttt tcttgatcat gaaaacgcca 181 acaaaattct gaatcggcca aagaggtata attcaggtaa attggaagag tttgttcaag 241 ggaaccttga gagagaatgt atggaagaaa agtgtagttt tgaagaagca cgagaagttt 301 ttgaaaacac tgaaagaaca actgaatttt ggaagcagta tgttgatgga gatcagtgtg 361 agtccaatcc atgtttaaat ggcggcagtt gcaaggatga cattaattcc tatgaatgtt 421 ggtgtccctt tggatttgaa ggaaagaact gtgaattaga tgtaacatgt aacattaaga 481 atggcagatg cgagcagttt tgtaaaaata gtgctgataa caaggtggtt tgctcctgta 541 ctgagggata tcgacttgca gaaaaccaga agtcctgtga accagcagtg ccatttccat 601 gtggaagagt ttctgtttca caaacttcta agctcacccg tgctgagact gtttttcctg 661 atgtggacta tgtaaattct actgaagctg aaaccatttt ggataacatc actcaaagca 721 cccaatcatt taatgacttc actcgggttg ttggtggaga agatgccaaa ccaggtcaat 781 tcccttggca ggttgttttg aatggtaaag ttgatgcatt ctgtggaggc tctatcgtta 841 atgaaaaatg gattgtaact gctgcccact gtgttgaaac tggtgttaaa attacagttg 901 tcgcaggtga acataatatt gaggagacag aacatacaga gcaaaagcga aatgtgattc 961 gaattattcc tcaccacaac tacaatgcag ctattaataa gtacaaccat gacattgccc 1021 ttctggaact ggacgaaccc ttagtgctaa acagctacgt tacacctatt tgcattgctg 1081 acaaggaata cacgaacatc ttcctcaaat ttggatctgg ctatgtaagt ggctggggaa 1141 gagtcttcca caaagggaga tcagctttag ttcttcagta ccttagagtt ccacttgttg 1201 accgagccac atgtcttcga tctacaaagt tcaccatcta taacaacatg ttctgtgctg 1261 gcttccatga aggaggtaga gattcatgtc aaggagatag tgggggaccc catgttactg 1321 aagtggaagg gaccagtttc ttaactggaa ttattagctg gggtgaagag tgtgcaatga 1381 aaggcaaata tggaatatat accaaggtat cccggtatgt caactggatt aaggaaaaaa 1441 caaagctcac tttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1501 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1561 caatcaacct ctggatta