Construct: ORF ccsbBroad304_00756

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002415.1_s304c1, BRDN0000461457
Derived from:
ccsbBroadEn_00756
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HMGCS2 (3158)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00756
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3158 HMGCS2 3-hydroxy-3-methylglutaryl-... NM_005518.4 100% 100%
2 human 3158 HMGCS2 3-hydroxy-3-methylglutaryl-... NM_001166107.1 91.7% 91.7% 558_559ins126
3 human 3158 HMGCS2 3-hydroxy-3-methylglutaryl-... XM_011541313.2 89.1% 89.1% 683_684ins165
4 mouse 15360 Hmgcs2 3-hydroxy-3-methylglutaryl-... NM_008256.4 86.3% 89.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1590
ORF length:
1524
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca gcgtctgttg actccagtga agcgcattct gcaactgaca agagcggtgc
121aggaaacctc cctcacacct gctcgcctgc tcccagtagc ccaccaaagg ttttctacag
181cctctgctgt ccccctggcc aaaacagata cttggccaaa ggacgtgggc atcctggccc
241tggaggtcta cttcccagcc caatatgtgg accaaactga cctggagaag tataacaatg
301tggaagcagg aaagtataca gtgggcttgg gccagacccg tatgggcttc tgctcagtcc
361aagaggacat caactccctg tgcctgacgg tggtgcaacg gctgatggag cgcatacagc
421tcccatggga ctctgtgggc aggctggaag taggcactga gaccatcatt gacaagtcca
481aagctgtcaa aacagtgctc atggaactct tccaggattc aggcaatact gatattgagg
541gcatagatac caccaatgcc tgctacggtg gtactgcctc cctcttcaat gctgccaact
601ggatggagtc cagttcctgg gatggtcgtt atgccatggt ggtctgtgga gacattgccg
661tctatcccag tggtaatgct cgtcccacag gtggggccgg agctgtggct atgctgattg
721ggcccaaggc ccctctggcc ctggagcgag ggctgagggg aacccatatg gagaatgtgt
781atgacttcta caaaccaaat ttggcctcgg agtacccaat agtggatggg aagctttcca
841tccagtgcta cttgcgggcc ttggatcgat gttacacatc ataccgtaaa aaaatccaga
901atcagtggaa gcaagctggc agcgatcgac ccttcaccct tgacgattta cagtacatga
961tctttcatac acccttttgc aagatggtcc agaagtctct ggctcgcctg atgttcaatg
1021acttcctgtc agccagcagt gacacacaaa ccagcttata taaggggctg gaggctttcg
1081gggggctaaa gctggaagac acctacacca acaaggacct ggataaagca cttctaaagg
1141cctctcagga catgttcgac aagaaaacca aggcttccct ttacctctcc actcacaatg
1201ggaacatgta cacctcatcc ctgtacgggt gcctggcctc gcttctgtcc caccactctg
1261cccaagaact ggctggctcc aggattggtg ccttctctta tggctctggt ttagcagcaa
1321gtttcttttc atttcgagta tcccaggatg ctgctccagg ctctcccctg gacaagttgg
1381tgtccagcac atcagacctg ccaaaacgcc tagcctcccg aaagtgtgtg tctcctgagg
1441agttcacaga aataatgaac caaagagagc aattctacca taaggtgaat ttctccccac
1501ctggtgacac aaacagcctt ttcccaggta cttggtacct ggagcgagtg gacgagcagc
1561atcgccgaaa gtatgcccgg cgtcccgtct acccaacttt cttgtacaaa gtggttggta
1621agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1681ggtggcgcgt taagtcgaca atcaacctct ggatta

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