Construct: ORF ccsbBroad304_00962
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017918.1_s304c1, BRDN0000405649
- Derived from:
- ccsbBroadEn_00962
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMAD4 (4089)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00962
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4089 | SMAD4 | SMAD family member 4 | NM_005359.6 | 100% | 100% | |
| 2 | mouse | 17128 | Smad4 | SMAD family member 4 | NM_008540.3 | 91.5% | 98.3% | (many diffs) |
| 3 | mouse | 17128 | Smad4 | SMAD family member 4 | XM_011246855.2 | 79.4% | 78.7% | (many diffs) |
| 4 | mouse | 17128 | Smad4 | SMAD family member 4 | XM_006525701.3 | 77.5% | 84.2% | (many diffs) |
| 5 | mouse | 17128 | Smad4 | SMAD family member 4 | NM_001364968.1 | 74.5% | 81.1% | (many diffs) |
| 6 | mouse | 17128 | Smad4 | SMAD family member 4 | NM_001364967.1 | 65.6% | 64.7% | (many diffs) |
| 7 | mouse | 17128 | Smad4 | SMAD family member 4 | NR_157374.1 | 15.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1722
- ORF length:
- 1656
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga caatatgtct attacgaata caccaacaag taatgatgcc tgtctgagca 121 ttgtgcatag tttgatgtgc catagacaag gtggagagag tgaaacattt gcaaaaagag 181 caattgaaag tttggtaaag aagctgaagg agaaaaaaga tgaattggat tctttaataa 241 cagctataac tacaaatgga gctcatccta gtaaatgtgt taccatacag agaacattgg 301 atgggaggct tcaggtggct ggtcggaaag gatttcctca tgtgatctat gcccgtctct 361 ggaggtggcc tgatcttcac aaaaatgaac taaaacatgt taaatattgt cagtatgcgt 421 ttgacttaaa atgtgatagt gtctgtgtga atccatatca ctacgaacga gttgtatcac 481 ctggaattga tctctcagga ttaacactgc agagtaatgc tccatcaagt atgatggtga 541 aggatgaata tgtgcatgac tttgagggac agccatcgtt gtccactgaa ggacattcaa 601 ttcaaaccat ccagcatcca ccaagtaatc gtgcatcgac agagacatac agcaccccag 661 ctctgttagc cccatctgag tctaatgcta ccagcactgc caactttccc aacattcctg 721 tggcttccac aagtcagcct gccagtatac tggggggcag ccatagtgaa ggactgttgc 781 agatagcatc agggcctcag ccaggacagc agcagaatgg atttactggt cagccagcta 841 cttaccatca taacagcact accacctgga ctggaagtag gactgcacca tacacaccta 901 atttgcctca ccaccaaaac ggccatcttc agcaccaccc gcctatgccg ccccatcccg 961 gacattactg gcctgttcac aatgagcttg cattccagcc tcccatttcc aatcatcctg 1021 ctcctgagta ttggtgttcc attgcttact ttgaaatgga tgttcaggta ggagagacat 1081 ttaaggttcc ttcaagctgc cctattgtta ctgttgatGG ATACGTGGAC CCTTCTGGAG 1141 GAGATCGCTT TTGTTTGGGT CAACTCTCCA ATGTCCACAG GACAGAAGCC ATTGAGAGAG 1201 CAAGGTTGCA CATAGGCAAA GGTGTGCAGT TGGAATGTAA AGGTGAAGGT GATGTTTGGG 1261 TCAGGTGCCT TAGTGACCAC GCGGTCTTTG TACAGAGTTA CTACTTAGAC AGAGAAGCTG 1321 GGCGTGCACC TGGAGATGCT GTTCATAAGA TCTACCCAAG TGCATATATA AAGGTCTTTG 1381 ATTTGCGTCA GTGTCATCGA CAGATGCAGC AGCAGGCGGC TACTGCACAA GCTGCAGCAG 1441 CTGCCCAGGC AGCAGCCGTG GCAGGAAACA TCCCTGGCCC AGGATCAGTA GGTGGAATAG 1501 CTCCAGCTAT CAGTCTGTCA GCTGCTGCTG GAATTGGTGT TGATGACCTT CGTCGCTTAT 1561 GCATACTCAG GATGAGTTTT GTGAAAGGCT GGGGACCGGA TTACCCAAGA CAGAGCATCA 1621 AAGAAACACC TTGCTGGATT GAAATTCACT TACACCGGGC CCTCCAGCTC CTAGACGAAG 1681 TACTTCATAC CATGCCGATT GCAGACCCAC AACCTTTAGA CTGCCCAACT TTCTTGTACa 1741 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1801 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta