Transcript: Human NM_005359.6

Homo sapiens SMAD family member 4 (SMAD4), mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
SMAD4 (4089)
Length:
8772
CDS:
539..2197

Additional Resources:

NCBI RefSeq record:
NM_005359.6
NBCI Gene record:
SMAD4 (4089)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005359.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000040029 CCTGAGTATTGGTGTTCCATT pLKO.1 1496 CDS 100% 4.950 6.930 N SMAD4 n/a
2 TRCN0000040031 CGAGTTGTATCACCTGGAATT pLKO.1 941 CDS 100% 0.000 0.000 N SMAD4 n/a
3 TRCN0000306807 CGAGTTGTATCACCTGGAATT pLKO_005 941 CDS 100% 0.000 0.000 N SMAD4 n/a
4 TRCN0000345740 TGCACCATACACACCTAATTT pLKO_005 1357 CDS 100% 15.000 12.000 N Smad4 n/a
5 TRCN0000040028 GCAGACAGAAACTGGATTAAA pLKO.1 2343 3UTR 100% 15.000 10.500 N SMAD4 n/a
6 TRCN0000288653 GCAGACAGAAACTGGATTAAA pLKO_005 2343 3UTR 100% 15.000 10.500 N SMAD4 n/a
7 TRCN0000025900 GCCAGCTACTTACCATCATAA pLKO.1 1306 CDS 100% 13.200 9.240 N Smad4 n/a
8 TRCN0000040030 GCTGCTGGAATTGGTGTTGAT pLKO.1 1997 CDS 100% 4.950 3.465 N SMAD4 n/a
9 TRCN0000010322 TATGCATACTCAGGATGAGTT pLKO.1 2031 CDS 100% 4.950 3.465 N SMAD4 n/a
10 TRCN0000010323 CAGATTGTCTTGCAACTTCAG pLKO.1 2789 3UTR 100% 4.050 2.835 N SMAD4 n/a
11 TRCN0000295871 CAGATTGTCTTGCAACTTCAG pLKO_005 2789 3UTR 100% 4.050 2.835 N SMAD4 n/a
12 TRCN0000040032 GTACTTCATACCATGCCGATT pLKO.1 2153 CDS 100% 4.050 2.835 N SMAD4 n/a
13 TRCN0000288654 GTACTTCATACCATGCCGATT pLKO_005 2153 CDS 100% 4.050 2.835 N SMAD4 n/a
14 TRCN0000010321 TACCATACAGAGAACATTGGA pLKO.1 754 CDS 100% 0.000 0.000 N SMAD4 n/a
15 TRCN0000295816 TACCATACAGAGAACATTGGA pLKO_005 754 CDS 100% 0.000 0.000 N SMAD4 n/a
16 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 4491 3UTR 100% 4.950 2.475 Y LOC387873 n/a
17 TRCN0000166364 CACACACACACACACACACAA pLKO.1 7743 3UTR 100% 4.950 2.475 Y KAAG1 n/a
18 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 4528 3UTR 100% 5.625 2.813 Y KLHL30 n/a
19 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 4528 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005359.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00962 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00962 pLX_304 36.6% 100% 100% V5 n/a
3 TRCN0000469053 TCGTCACACAGCCCGGTGTTGGGA pLX_317 18.6% 100% 100% V5 n/a
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