Transcript: Mouse XM_011246855.2

PREDICTED: Mus musculus SMAD family member 4 (Smad4), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Smad4 (17128)
Length:
7281
CDS:
212..1657

Additional Resources:

NCBI RefSeq record:
XM_011246855.2
NBCI Gene record:
Smad4 (17128)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011246855.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000345664 ATCTACCCAAGCGCGTATATA pLKO_005 1493 CDS 100% 15.000 21.000 N Smad4 n/a
2 TRCN0000362660 ATCCAACACCCGCCAAGTAAT pLKO_005 752 CDS 100% 13.200 18.480 N Smad4 n/a
3 TRCN0000362661 ATGAGTACGTTCACGACTTTG pLKO_005 687 CDS 100% 10.800 15.120 N Smad4 n/a
4 TRCN0000025953 CCATATCACTATGAGCGGGTT pLKO.1 599 CDS 100% 2.160 3.024 N Smad4 n/a
5 TRCN0000345740 TGCACCATACACACCTAATTT pLKO_005 1027 CDS 100% 15.000 12.000 N Smad4 n/a
6 TRCN0000025900 GCCAGCTACTTACCATCATAA pLKO.1 976 CDS 100% 13.200 9.240 N Smad4 n/a
7 TRCN0000345739 TCAGGTAGGAGAGACGTTTAA pLKO_005 1207 CDS 100% 13.200 9.240 N Smad4 n/a
8 TRCN0000025881 GCTTACTTTGAAATGGACGTT pLKO.1 1187 CDS 100% 2.640 1.848 N Smad4 n/a
9 TRCN0000025885 GCGATTGTGCATTCTCAGGAT pLKO.1 1710 3UTR 100% 2.640 1.584 N Smad4 n/a
10 TRCN0000345738 GCGATTGTGCATTCTCAGGAT pLKO_005 1710 3UTR 100% 2.640 1.584 N Smad4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011246855.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00962 pDONR223 100% 79.4% 78.7% None (many diffs) n/a
2 ccsbBroad304_00962 pLX_304 36.6% 79.4% 78.7% V5 (many diffs) n/a
3 TRCN0000469053 TCGTCACACAGCCCGGTGTTGGGA pLX_317 18.6% 79.4% 78.7% V5 (many diffs) n/a
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