Construct: ORF ccsbBroad304_01012

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002155.1_s304c1, BRDN0000403936
Derived from:
ccsbBroadEn_01012
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MID1 (4281)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01012
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4281 MID1 midline 1 NM_000381.4 100% 100%
2 human 4281 MID1 midline 1 NM_001098624.2 100% 100%
3 human 4281 MID1 midline 1 NM_001193277.1 100% 100%
4 human 4281 MID1 midline 1 NM_001347733.2 100% 100%
5 human 4281 MID1 midline 1 NM_033290.4 100% 100%
6 human 4281 MID1 midline 1 NM_033289.2 94.3% 94.3% 542_543ins114
7 human 4281 MID1 midline 1 NM_001193279.1 70.4% 63.7% (many diffs)
8 human 4281 MID1 midline 1 NM_001193278.1 65.4% 59.2% (many diffs)
9 human 4281 MID1 midline 1 NM_001193280.1 64.7% 58.1% (many diffs)
10 human 4281 MID1 midline 1 NM_001193281.1 33.1% 32.6% (many diffs)
11 mouse 17318 Mid1 midline 1 NM_001290504.1 88.8% 95.3% (many diffs)
12 mouse 17318 Mid1 midline 1 NM_001290505.1 88.8% 95.3% (many diffs)
13 mouse 17318 Mid1 midline 1 XM_011247789.3 88.8% 95.3% (many diffs)
14 mouse 17318 Mid1 midline 1 XM_030251241.1 88.8% 95.3% (many diffs)
15 mouse 17318 Mid1 midline 1 NM_010797.3 87.1% 93.3% (many diffs)
16 mouse 17318 Mid1 midline 1 XM_017318408.2 87.1% 93.3% (many diffs)
17 mouse 17318 Mid1 midline 1 XM_017318409.2 87.1% 93.3% (many diffs)
18 mouse 17318 Mid1 midline 1 XM_017318410.2 87.1% 93.3% (many diffs)
19 mouse 17318 Mid1 midline 1 XM_030251242.1 83.9% 89.9% (many diffs)
20 mouse 17318 Mid1 midline 1 XM_030251243.1 83.9% 89.9% (many diffs)
21 mouse 17318 Mid1 midline 1 XM_017318413.2 83.8% 90% (many diffs)
22 mouse 17318 Mid1 midline 1 NM_001290506.1 83.1% 89.6% (many diffs)
23 mouse 17318 Mid1 midline 1 XM_030251244.1 83.1% 89.6% (many diffs)
24 mouse 17318 Mid1 midline 1 XM_017318411.2 82.3% 88% (many diffs)
25 mouse 17318 Mid1 midline 1 XM_017318412.2 81.5% 87.7% (many diffs)
26 mouse 17318 Mid1 midline 1 NM_001290512.1 59.6% 63.2% (many diffs)
27 mouse 17318 Mid1 midline 1 XM_006528737.4 50% 52.1% (many diffs)
28 mouse 17318 Mid1 midline 1 XM_006528736.4 49% 51% (many diffs)
29 mouse 17318 Mid1 midline 1 XR_878259.3 43.2% (many diffs)
30 mouse 17318 Mid1 midline 1 XM_006528739.4 41% 44.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2067
ORF length:
2001
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga aacactggag tcagaactga cctgccctat ttgtctggag ctctttgagg
121accctcttct actgccctgc gcacacagcc tctgcttcaa ctgcgcccac cgcatcctag
181tatcacactg tgccaccaac gagtctgtgg agtccatcac cgccttccag tgccccacct
241gccggcatgt catcaccctc agccagcgag gtctagacgg gctcaagcgc aacgtcaccc
301tacagaacat catcgacagg ttccagaaag catcagtgag cgggcccaac tctcccagcg
361agacccgtcg ggagcgggcc tttgacgcca acaccatgac ctccgccgag aaggtcctct
421gccagttttg tgaccaggat cctgcccagg acgctgtgaa gacctgtgtc acttgtgaag
481tatcctactg tgacgagtgc ctgaaagcca ctcacccgaa taagaagccc tttacaggcc
541atcgtctgat tgagccaatt ccggactctc acatccgggg gctgatgtgc ttggagcatg
601aggatgagaa ggtgaatatg tactgtgtga ccgatgacca gttaatctgt gccttgtgta
661aactggttgg gcggcaccgc gatcatcagg tggcagcttt gagtgagcgc tatgacaaat
721tgaagcaaaa cttagagagt aacctcacca accttattaa gaggaacaca gaactggaga
781cccttttggc taaactcatc caaacctgtc aacatgttga agtcaatgca tcacgtcaag
841aagccaaatt gacagaggag tgtgatcttc tcattgagat cattcagcaa agacgacaga
901ttattggaac caagatcaaa gaagggaagg tgatgaggct tcgcaaactg gctcagcaga
961ttgcaaactg caaacagtgc attgagcggt cagcatcact catctcccaa gcggaacact
1021ctctgaagga gaatgatcat gcgcgtttcc tacagactgc taagaatatc accgagagag
1081tctccatggc aactgcatcc tcccaggttc taattcctga aatcaacctc aatgacacat
1141ttgacacctt tgccttagat ttttcccgag agaagaaact gctagaatgt ctggattacc
1201ttacagctcc caaccctccc acaattagag aagagctctg cacagcttca tatgacacca
1261tcactgtgca ttggacctcc gatgatgagt tcagcgtggt ctcctacgag ctccagtaca
1321ccatattcac cggacaagcc aacgtcgtta gtctgtgtaa ttcggctgat agctggatga
1381tagtacccaa catcaagcag aaccactaca cggtgcacgg tctgcagagc ggcaccaagt
1441acatcttcat ggtcaaggcc atcaaccagg cgggcagccg cagcagtgag cctgggaagt
1501tgaagacaaa cagccaacca tttaaactgg atcccaaatc tgctcatcga aaactgaagg
1561tgtcccatga taacttgaca gtagaacgtg atgagtcatc atccaagaag agtcacacac
1621ctgaacgctt caccagccag gggagctatg gagtagctgg aaatgtgttt attgatagtg
1681gccggcatta ttgggaagtg gtcataagtg gaagcacatg gtatgccatt ggtcttgctt
1741acaaatcagc cccgaagcat gaatggattg ggaagaactc tgcttcctgg gcgctctgcc
1801gctgcaacaa taactgggtg gtgagacaca atagcaagga aatccccatt gagcctgccc
1861cccacctccg gcgcgtgggc atcctgctgg actatgataa cggctctatc gccttttatg
1921atgctttgaa ctccatccac ctctacacct tcgacgtcgc atttgcgcag cctgtttgcc
1981ccaccttcac cgtgtggaac aagtgtctga cgattatcac tgggctccct atcccagacc
2041atttggactg cacagagcag ctgccgtgcc caactttctt gtacaaagtg gttggtaagc
2101ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
2161ggcgcgttaa gtcgacaatc aacctctgga tta

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