Construct: ORF ccsbBroad304_01094
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016809.1_s304c1, BRDN0000398951
- Derived from:
- ccsbBroadEn_01094
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFKBIB (4793)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01094
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4793 | NFKBIB | NFKB inhibitor beta | NM_002503.5 | 100% | 100% | |
| 2 | human | 4793 | NFKBIB | NFKB inhibitor beta | NM_001369699.1 | 92.1% | 91.5% | (many diffs) |
| 3 | human | 4793 | NFKBIB | NFKB inhibitor beta | XM_006723226.2 | 91% | 76.2% | 0_1ins40;139_140ins56 |
| 4 | human | 4793 | NFKBIB | NFKB inhibitor beta | XM_006723227.3 | 82.8% | 75.1% | (many diffs) |
| 5 | human | 4793 | NFKBIB | NFKB inhibitor beta | NM_001243116.1 | 75.8% | 75.8% | 0_1ins258 |
| 6 | human | 4793 | NFKBIB | NFKB inhibitor beta | NR_161470.1 | 74.4% | (many diffs) | |
| 7 | human | 4793 | NFKBIB | NFKB inhibitor beta | NM_001369700.1 | 74.1% | 74.1% | 283_284ins276 |
| 8 | human | 4793 | NFKBIB | NFKB inhibitor beta | NR_040515.2 | 42.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1134
- ORF length:
- 1068
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tggggtcgcg tgcttgggaa aagctgccga cgcagatgaa tggtgcgaca 121 gcggcctggg ctccctgggt ccggacgcag cggcccccgg aggacctggg ttgggcgcgg 181 agttgggccc ggggctgtcg tgggctcccc tcgtcttcgg ctacgtcact gaggatgggg 241 acacggcact gcacttggct gtgattcatc agcatgaacc cttcctggat tttcttctag 301 gcttctcggc cggcactgag tacatggacc tgcagaatga cctaggccag acagccctgc 361 acctggcagc catcctgggg gagacatcca cggtggagaa gctgtacgca gcaggcgccg 421 ggctgtgtgt ggcggagcgt aggggccaca cggcgctgca cctggcctgc cgtgtggggg 481 cacacgcctg tgcccgtgcc ctgcttcagc cccgcccccg gcgccccagg gaagcccccg 541 acacctacct cgctcagggc cctgaccGTA CTCCCGACAC CAACCATACC CCTGTCGCCT 601 TGTACCCCGA TTCCGACTTG GAGAAGGAAG AAGAGGAGAG TGAGGAGGAC TGGAAGCTGC 661 AGCTGGAGGC TGAAAACTAC GAGGGCCACA CCCCACTCCA CGTGGCCGTT ATCCACAAAG 721 ATGTGGAGAT GGTCCGGCTG CTCCGAGATG CTGGAGCTGA CCTTGACAAA CCGGAGCCCA 781 CGTGCGGCCG GAGCCCCCTT CATTTGGCAG TGGAGGCCCA GGCAGCCGAT GTGCTGGAGC 841 TTCTCCTGAG GGCAGGCGCG AACCCTGCTG CCCGCATGTA CGGTGGCCGC ACCCCACTCG 901 GCAGTGCCAT GCTCCGGCCC AACCCCATCC TCGCCCGCCT CCTCCGTGCA CACGGAGCCC 961 CTGAGCCCGA GGGCGAGGAC GAGAAATCCG GCCCCTGCAG CAGCAGTAGC GACAGCGACA 1021 GCGGAGACGA GGGCGATGAA TACGACGACA TTGTGGTTCA CAGCAGCCGC AGCCAAACCC 1081 GGCTGCCTCC CACCCCAGCC TCAAAACCTC TTCCTGACGA CCCCCGCCCC GTGTGCCCAA 1141 CTTTCTTGTA Caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1201 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta