Construct: ORF ccsbBroad304_01144
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011384.1_s304c1, BRDN0000411750
- Derived from:
- ccsbBroadEn_01144
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAK1 (5058)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01144
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | NM_002576.4 | 100% | 100% | |
| 2 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017846.1 | 100% | 100% | |
| 3 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017847.2 | 100% | 100% | |
| 4 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017848.1 | 100% | 100% | |
| 5 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017849.1 | 100% | 100% | |
| 6 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_017017850.1 | 100% | 100% | |
| 7 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448560.1 | 100% | 100% | |
| 8 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448561.1 | 100% | 100% | |
| 9 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448562.1 | 100% | 100% | |
| 10 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448563.1 | 100% | 100% | |
| 11 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448564.1 | 100% | 100% | |
| 12 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | NM_001128620.1 | 95.6% | 94.4% | 1551_1599del;1659_1660ins25 |
| 13 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545080.3 | 95.6% | 94.4% | 1551_1599del;1659_1660ins25 |
| 14 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545081.2 | 95.6% | 94.4% | 1551_1599del;1659_1660ins25 |
| 15 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545082.2 | 95.6% | 94.4% | 1551_1599del;1659_1660ins25 |
| 16 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545083.2 | 95.6% | 94.4% | 1551_1599del;1659_1660ins25 |
| 17 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_011545084.3 | 95.6% | 94.4% | 1551_1599del;1659_1660ins25 |
| 18 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448557.1 | 95.6% | 94.4% | 1551_1599del;1659_1660ins25 |
| 19 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448558.1 | 95.6% | 94.4% | 1551_1599del;1659_1660ins25 |
| 20 | human | 5058 | PAK1 | p21 (RAC1) activated kinase 1 | XM_024448559.1 | 95.6% | 94.4% | 1551_1599del;1659_1660ins25 |
| 21 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | NM_011035.2 | 92.1% | 99% | (many diffs) |
| 22 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_017322028.1 | 92.1% | 99% | (many diffs) |
| 23 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_006507434.1 | 90.8% | 97.6% | (many diffs) |
| 24 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_006507436.3 | 90.8% | 97.6% | (many diffs) |
| 25 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_006507437.2 | 90.8% | 97.6% | (many diffs) |
| 26 | mouse | 18479 | Pak1 | p21 protein (Cdc42/Rac)-act... | XM_011241694.1 | 90.8% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1704
- ORF length:
- 1635
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcaaataac ggcctagaca ttcaagacaa acccccagcc cctccgatga 121 gaaataccag cactatgatt ggagccggca gcaaagatgc tggaacccta aaccatggtt 181 ctaaacctct gcctccaaac ccagaggaga agaaaaagaa ggaccgattt taccgatcca 241 ttttacctgg agataaaaca aataaaaaga aagagaaaga gcggccagag atttctctcc 301 cttcagattt tgaacacaca attcatgtcg gttttgatgc tgtcacaggg gagtttacgg 361 gaatgccaga gcagtgggcc cgcttgcttc agacatcaaa tatcactaag tcggagcaga 421 agaaaaaccc gcaggctgtt ctggatgtgt tggagtttta caactcgaag aagacatcca 481 acagccagaa atacatgagc tttacagata agtcagctga ggattacaat tcttctaatg 541 ccttgaatgt gaaggctgtg tctgagactc ctgcagtgcc accagtttca gaagatgagg 601 atgatgatga tgatgatgct accccaccac cagtgattgc tccacgccca gagcacacaa 661 aatctgtata cacacggtct gtgattgaac cacttcctgt cactccaact cgggacgtgg 721 ctacatctcc catttcacct actgaaaata acaccactcc accagatgct ttgacccgga 781 atactgagaa gcagaagaag aagcctaaaa tgtctgatga ggagatcttg gagaaattac 841 gaagcatagt gagtgtgggc gatcctaaga agaaatatac acggtttgag aagattggac 901 aaggtgcttc aggcaccgtg tacacagcaa tggatgtggc cacaggacag gaggtggcca 961 ttaagcagat gaatcttcag cagcagccca agaaagagct gattattaat gagatcctgg 1021 tcatgaggga aaacaagaac ccaaacattg tgaattactt ggacagttac ctcgtgggag 1081 atgagctgtg ggttgttatg gaatacttgg ctggaggctc cttgacagat gtggtgacag 1141 aaacttgcat ggatgaaggc caaattgcag ctgtgtgccg tgagtgtctg caggctcTGG 1201 AGTTCTTGCA TTCGAACCAG GTCATTCACA GAGACATCAA GAGTGACAAT ATTCTGTTGG 1261 GAATGGATGG CTCTGTCAAG CTAACTGACT TTGGATTCTG TGCACAGATA ACCCCAGAGC 1321 AGAGCAAACG GAGCACCATG GTAGGAACCC CATACTGGAT GGCACCAGAG GTTGTGACAC 1381 GAAAGGCCTA TGGGCCCAAG GTTGACATCT GGTCCCTGGG CATCATGGCC ATCGAAATGA 1441 TTGAAGGGGA GCCTCCATAC CTCAATGAAA ACCCTCTGAG AGCCTTGTAC CTCATTGCCA 1501 CCAATGGGAC CCCAGAACTT CAGAACCCAG AGAAGCTGTC AGCTATCTTC CGGGACTTTC 1561 TGAACCGCTG TCTCGAGATG GATGTGGAGA AGAGAGGTTC AGCTAAAGAG CTGCTACAGC 1621 ATCAATTCCT GAAGATTGCC AAGCCCCTCT CCAGCCTCAC TCCACTGATT GCTGCAGCTA 1681 AGGAGGCAAC AAAGAACAAT CACTTGCCAA CTTTCTTGTA Caaagtggtt ggtaagccta 1741 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1801 gcgttaagtc gacaatcaac ctctggatta