Transcript: Mouse NM_011035.2

Mus musculus p21 protein (Cdc42/Rac)-activated kinase 1 (Pak1), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Pak1 (18479)
Length:
3081
CDS:
228..1862

Additional Resources:

NCBI RefSeq record:
NM_011035.2
NBCI Gene record:
Pak1 (18479)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145359 GGGCGTGGAGCAATCACTGG pXPR_003 AGG 561 34% 6 -0.0504 Pak1 PAK1 76466
2 BRDN0001145958 TTCTGTCACCACATCTGTCA pXPR_003 AGG 1054 64% 11 -0.6815 Pak1 PAK1 76464
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_011035.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000025256 CCGAAGAAAGAGCTGATTATT pLKO.1 1143 CDS 100% 15.000 12.000 N Pak1 n/a
2 TRCN0000433563 GTTTGATAGCATTATCGATTT pLKO_005 2209 3UTR 100% 10.800 8.640 N Pak1 n/a
3 TRCN0000417541 AGTCAGCTGAAGATTATAATT pLKO_005 670 CDS 100% 15.000 10.500 N Pak1 n/a
4 TRCN0000423197 ACATCAAGAGTGACAATATTC pLKO_005 1390 CDS 100% 13.200 9.240 N Pak1 n/a
5 TRCN0000423643 GTGCTTCAGGCACAGTGTATA pLKO_005 1060 CDS 100% 13.200 9.240 N Pak1 n/a
6 TRCN0000417442 ACCATGGTGGGAACTCCATAT pLKO_005 1491 CDS 100% 10.800 7.560 N Pak1 n/a
7 TRCN0000434705 ACTCTAAGAAGACCTCCAATA pLKO_005 622 CDS 100% 10.800 7.560 N Pak1 n/a
8 TRCN0000025258 GCTGTGGGTTGTTATGGAATA pLKO.1 1241 CDS 100% 10.800 7.560 N Pak1 n/a
9 TRCN0000025254 CCCAGAGAAGTTGTCAGCTAT pLKO.1 1682 CDS 100% 4.950 3.465 N Pak1 n/a
10 TRCN0000002227 CTTCTCCCATTTCCTGATCTA pLKO.1 1959 3UTR 100% 4.950 2.970 N PAK1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_011035.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01144 pDONR223 100% 92.1% 99% None (many diffs) n/a
2 ccsbBroad304_01144 pLX_304 31.1% 92.1% 99% V5 (many diffs) n/a
3 ccsbBroadEn_14726 pDONR223 84.3% 91.5% 40.1% None (many diffs) n/a
4 TRCN0000488535 ATAGGCAGGATTGATTGGACAGGA pLX_317 18.3% 89.4% 93.5% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489311 GAGCCAGCTGAAAACATACTTGAC pLX_317 18.9% 88.3% 93.6% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000492322 TACACAATCAATTACGAAATACCT pLX_317 31% 75.9% 81.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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