Construct: ORF ccsbBroad304_01216
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013941.1_s304c1, BRDN0000411318
- Derived from:
- ccsbBroadEn_01216
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLD1 (5337)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01216
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5337 | PLD1 | phospholipase D1 | NM_002662.5 | 100% | 100% | |
| 2 | human | 5337 | PLD1 | phospholipase D1 | XM_005247533.2 | 100% | 100% | |
| 3 | human | 5337 | PLD1 | phospholipase D1 | NM_001130081.3 | 96.4% | 96.3% | 1753_1754ins114 |
| 4 | human | 5337 | PLD1 | phospholipase D1 | XM_005247534.2 | 96.4% | 96.3% | 1753_1754ins114 |
| 5 | human | 5337 | PLD1 | phospholipase D1 | XM_011512897.1 | 90% | 89.6% | (many diffs) |
| 6 | human | 5337 | PLD1 | phospholipase D1 | XM_011512898.1 | 89.9% | 89.5% | (many diffs) |
| 7 | human | 5337 | PLD1 | phospholipase D1 | XM_017006623.2 | 86.5% | 86% | (many diffs) |
| 8 | human | 5337 | PLD1 | phospholipase D1 | XM_024453598.1 | 62.1% | 62.1% | 0_1ins1104;649_650ins114 |
| 9 | human | 5337 | PLD1 | phospholipase D1 | XM_011512899.2 | 61.1% | 61.1% | 0_1ins1251 |
| 10 | human | 5337 | PLD1 | phospholipase D1 | XM_017006624.1 | 61.1% | 61.1% | 0_1ins1251 |
| 11 | human | 5337 | PLD1 | phospholipase D1 | XR_001740175.1 | 51.4% | 1_196del;2426_2593del;3587_6263del | |
| 12 | human | 5337 | PLD1 | phospholipase D1 | XR_001740174.1 | 50.4% | (many diffs) | |
| 13 | mouse | 18805 | Pld1 | phospholipase D1 | XM_006535414.3 | 84.9% | 91.1% | (many diffs) |
| 14 | mouse | 18805 | Pld1 | phospholipase D1 | XM_017319489.1 | 84.9% | 91.1% | (many diffs) |
| 15 | mouse | 18805 | Pld1 | phospholipase D1 | XM_017319490.1 | 84.9% | 91.1% | (many diffs) |
| 16 | mouse | 18805 | Pld1 | phospholipase D1 | XM_017319491.1 | 84.9% | 91.1% | (many diffs) |
| 17 | mouse | 18805 | Pld1 | phospholipase D1 | NM_001164056.1 | 81.8% | 88.1% | (many diffs) |
| 18 | mouse | 18805 | Pld1 | phospholipase D1 | NM_008875.4 | 81.8% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3288
- ORF length:
- 3222
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc actgaaaaac gagccacggg taaatacctc tgcactgcag aaaattgctg 121 ctgacatgag taatatcata gaaaatctgg acacgcggga actccacttt gagggagagg 181 aggtagacta cgacgtgtct cccagcgatc ccaagataca agaagtgtat atccctttct 241 ctgctattta taacactcaa ggatttaagg agcctaatat acagacgtat ctctccggct 301 gtccaataaa agcacaagtt ctggaagtgg aacgcttcac atctacaaca agggtaccaa 361 gtattaatct ttacactatt gaattaacac atggggaatt taaatggcaa gttaagagga 421 aattcaagca ttttcaagaa tttcacagag agctgctcaa gtacaaagcc tttatccgca 481 tccccattcc cactagaaga cacacgttta ggaggcaaaa cgtcagagag gagcctcgag 541 agatgcccag tttgccccgt tcatctgaaa acatgataag agaagaacaa ttccttggta 601 gaagaaaaca actggaagat tacttgacaa agatactaaa aatgcccatg tatagaaact 661 atcatgccac aacagagttt cttgatataa gccagctgtc tttcatccat gatttgggac 721 caaagggcat agaaggtatg ataatgaaaa gatctggagg acacagaata ccaggcttga 781 attgctgtgg tcagggaaga gcctgctaca gatggtcaaa aagatggtta atagtgaaag 841 attccttttt attgtatatg aaaccagaca gcggtgccat tgccttcgtc ctgctggtag 901 acaaagaatt caaaattaag gtggggaaga aggagacaga aacgaaatat ggaatccgaa 961 ttgataatct ttcaaggaca cttattttaa aatgcaacag ctatagacat gctcggtggt 1021 ggggaggggc tatagaagaa ttcatccaga aacatggcac caactttctc aaagatcatc 1081 gatttgggtc atatgctgct atccaagaga atgctttagc taaatggtat gttaatgcca 1141 aaggatattt tgaagatgtg gcaaatgcaa tggaagaggc aaatgaagag atttttatca 1201 cagactggtg gctgagtcca gaaatcttcc tgaaacgccc agtggttgag ggaaatcgtt 1261 ggaggttgga ctgcattctt aaacgaaaag cacaacaagg agtgaggatc ttcataatgc 1321 tctacaaaga ggtggaactc gctcttggca tcaatagtga atacaccaag aggactttga 1381 tgcgtctaca tcccaacata aaggtgatga gacacccgga tcatgtgtca tccaccgtct 1441 atttgtgggc tcaccatgag aagcttgtca tcattgacca atcggtggcc tttgtgggag 1501 ggattgacct ggcctatgga aggtgggacg acaatgagca cagactcaca gacgtgggca 1561 gtgtgaagcg ggtcacttca ggaccgtctc tgggttccct cccacctgcc gcaatggagt 1621 ctatggaatc cttaagactc aaagataaaa atgagcctgt tcaaaaccta cccatccaga 1681 agagtattga tgatgtggat tcaaaactga aaggaatagg aaagccaaga aagttctcca 1741 aatttagtct ctacaagcag ctccacaggc accacctgca cgacgcagat agcatcagca 1801 gcattgacag cacctccagt tattttaatc actatagaag tcatcacaat ttaatccatg 1861 gtttaaaacc ccacttcaaa ctctttcacc cgtccagtga gtctgagcaa ggactcacta 1921 gacctcatgc tgataccggg tccatccgta gtttacagac aggtgtggga gagctgcatg 1981 gggaaaccag attctggcat ggaaaggact actgcaattt cgtcttcaaa gactgggttc 2041 aacttgataa accttttgct gatttcattg acaggtactc cacgccccgg atgccctggc 2101 atgacattgc ctctgcagtc cacgggaagg cggctcgtga tgtggcacgt cacttcatcc 2161 agcgctggaa cttcacaaaa attatgaaat caaaatatcg gtccctttct tatccttttc 2221 tgcttccaaa gtctcaaaca acagcccatg agttgagata tcaagtgcct gggtctgtcc 2281 atgctaacgt acagttgctc cgctctgctg ctgattggtc tgctggtata aagtaccatg 2341 aagagtccat ccacgccgct tacgtccatg tgatagagaa cagcaggcac tatatctata 2401 tcgaaaacca gtttttcata agctgtgctg atgacaaagt tgtgttcaac aagataggcg 2461 atgccattgc ccagaggatc ctgaaagctc acagggaaaa ccagaaatac cgggtatatg 2521 tcgtgatacc acttctgcca gggttcgaag gagacatttc aaccggcgga ggaaatgctc 2581 tacaggcaat catgcacttc aactacagaa ccatgtgcag aggagaaaat tccatccttg 2641 gacagttaaa agcagagctt ggtaatcagt ggataaatta catatcattc tgtggtctta 2701 gaacacatgc agagctcgaa ggaaacctag taactgagct tatctatgtc cacagcaagt 2761 tgttaattgc tgatgataac actgttatta ttggctctgc caacataaat gaccgcagca 2821 tgctgggaaa gcgtgacagt gaaatggctg tcattgtgca agatacagag actgttcctt 2881 cagtaatgga tggaaaagag taccaagctg gccggtttgc ccgaggactt cggctacagt 2941 gctttagggt tgtccttggc tatcttgatg acccaagtga ggacattcag gatccagtga 3001 gtgacaaatt cttcaaggag gtgtgggttt caacagcagc tcgaaatgct acaatttatg 3061 acaaggtttt ccggtgcctt cccaatgatg aagtacacaa tttaattcag ctgagagact 3121 ttataaacaa gcccgtatta gctaaggaag atcccattcg agctgaggag gaactgaaga 3181 agatccgtgg atttttggtg caattcccct tttatttctt gtctgaagaa agcctactgc 3241 cttctgttgg gaccaaagag gccatagtgc ccatggaggt ttggacttac ccaactttct 3301 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 3361 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta