Transcript: Mouse NM_001164056.1

Mus musculus phospholipase D1 (Pld1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Pld1 (18805)
Length:
4987
CDS:
253..3363

Additional Resources:

NCBI RefSeq record:
NM_001164056.1
NBCI Gene record:
Pld1 (18805)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001164056.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000348897 GCTCGAAACGCTACCATATAT pLKO_005 3112 CDS 100% 15.000 21.000 N Pld1 n/a
2 TRCN0000375650 CAGTGGTCACCCTGATATAAG pLKO_005 3501 3UTR 100% 13.200 18.480 N Pld1 n/a
3 TRCN0000379272 TATGGACTTCGGATCGATAAT pLKO_005 1135 CDS 100% 13.200 18.480 N Pld1 n/a
4 TRCN0000076819 CCCATCTCAAAGATTGTTGAT pLKO.1 1858 CDS 100% 4.950 6.930 N Pld1 n/a
5 TRCN0000076822 GCTCTCCATGAGAACACGTTA pLKO.1 1285 CDS 100% 4.950 3.960 N Pld1 n/a
6 TRCN0000076818 GCTGCCTACATCCATGTAATA pLKO.1 2428 CDS 100% 13.200 9.240 N Pld1 n/a
7 TRCN0000076821 GCTTGGTAATAAGTGGATAAA pLKO.1 2730 CDS 100% 13.200 9.240 N Pld1 n/a
8 TRCN0000352178 GCTTGGTAATAAGTGGATAAA pLKO_005 2730 CDS 100% 13.200 9.240 N Pld1 n/a
9 TRCN0000001009 GCAAGTTAAGAGGAAATTCAA pLKO.1 594 CDS 100% 5.625 3.938 N PLD1 n/a
10 TRCN0000076820 CCCAATGATGAAGTACACAAT pLKO.1 3154 CDS 100% 4.950 3.465 N Pld1 n/a
11 TRCN0000352251 CCCAATGATGAAGTACACAAT pLKO_005 3154 CDS 100% 4.950 3.465 N Pld1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001164056.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01216 pDONR223 100% 81.8% 88.1% None (many diffs) n/a
2 ccsbBroad304_01216 pLX_304 0% 81.8% 88.1% V5 (many diffs) n/a
3 TRCN0000470850 GCCGCTAACCCCAAAACTTCAATT pLX_317 12.6% 81.8% 88.1% V5 (many diffs) n/a
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