Construct: ORF ccsbBroad304_01271
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006752.1_s304c1, BRDN0000410025
- Derived from:
- ccsbBroadEn_01271
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPT1 (5538)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01271
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5538 | PPT1 | palmitoyl-protein thioester... | NM_000310.3 | 100% | 100% | |
| 2 | human | 5538 | PPT1 | palmitoyl-protein thioester... | NM_001363695.2 | 92.1% | 92.1% | 724_725ins72 |
| 3 | human | 5538 | PPT1 | palmitoyl-protein thioester... | NM_001142604.2 | 66.3% | 66.3% | 123_124ins309 |
| 4 | mouse | 19063 | Ppt1 | palmitoyl-protein thioester... | NM_008917.3 | 84.8% | 85.2% | (many diffs) |
| 5 | mouse | 19063 | Ppt1 | palmitoyl-protein thioester... | XR_376299.3 | 27.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 984
- ORF length:
- 918
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcgcccggc tgcctgtggc tcttggctgt ggctctcctg ccatggacct 121 gcgcttctcg ggcgctgcag catctggacc cgccggcgcc gctgccgttg gtgatctggc 181 atgggatggg agacagctgt tgcaatccct taagcatggg tgctattaaa aaaatggtgg 241 agaagaaaat acctggaatt tacgtcttat ctttagagat tgggaagacc ctgatggagg 301 acgtggagaa cagcttcttc ttgaatgtca attcccaagt aacaacagtg tgtcaggcac 361 ttgctaagga tcctaaattg cagcaaggct acaatgctat gggattctcc cagggaggcc 421 aatttctgag ggcagtggct cagagatgcc cttcacctcc catgatcaat ctgatctcgg 481 ttgggggaca acatcaaggt gtttttggac tccctcgatg cccaggagag agctctcaca 541 tctgtgactt catccgaaaa acactgaatg ctggggcgta ctccaaagtt gttcaggaac 601 gcctcgtgca agccgaatac tggcatgacc ccataaagga ggatgtgtat cgcaaccaca 661 gcatcttctt ggcagatata aatcaggagc ggggtatcaa tgagtcctac aagaaaaacc 721 tgatggccct gaagaagttt gtgatggtga aattcctcaa tgattccatt gtggaccctg 781 tagattcgga gtggtttgga ttttacagaa gtggccaagc caaggaaacc attcccttac 841 aggagacctc cctgtacaca caggaccgcc tggggctaaa ggaaatggac aatgcaggac 901 agctagtgtt tctggctaca gaaggggacc atcttcagtt gtctgaagaa tggttttatg 961 cccacatcat accattcctt ggatgcccaa ctttcttgta caaagtggtt ggtaagccta 1021 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1081 gcgttaagtc gacaatcaac ctctggatta