Construct: ORF ccsbBroad304_01290

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005531.1_s304c1, BRDN0000400092
Derived from:
ccsbBroadEn_01290
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAP2K5 (5607)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01290
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5607 MAP2K5 mitogen-activated protein k... NM_145160.3 100% 100%
2 human 5607 MAP2K5 mitogen-activated protein k... NM_002757.4 97.7% 97.7% 1042_1043ins30
3 human 5607 MAP2K5 mitogen-activated protein k... NM_001206804.2 91.3% 90.2% (many diffs)
4 human 5607 MAP2K5 mitogen-activated protein k... XM_024449988.1 82.8% 82.8% 0_1ins231
5 human 5607 MAP2K5 mitogen-activated protein k... XM_011521787.3 64.2% 63.6% (many diffs)
6 human 5607 MAP2K5 mitogen-activated protein k... XM_011521788.3 63% 62.9% 848_849delGGins497
7 human 5607 MAP2K5 mitogen-activated protein k... XM_017022414.2 59.3% 59.3% 797_798delCA;801_802ins545
8 human 5607 MAP2K5 mitogen-activated protein k... XR_001751355.2 48.6% 1_651del;1449_1450ins49;1621_1622ins325
9 mouse 23938 Map2k5 mitogen-activated protein k... NM_011840.2 91% 97.7% (many diffs)
10 mouse 23938 Map2k5 mitogen-activated protein k... XM_006511145.2 89% 95.7% (many diffs)
11 mouse 23938 Map2k5 mitogen-activated protein k... XM_006511146.2 88.8% 95.5% (many diffs)
12 mouse 23938 Map2k5 mitogen-activated protein k... XM_011242740.2 85.3% 91% (many diffs)
13 mouse 23938 Map2k5 mitogen-activated protein k... XM_011242741.2 72.7% 78.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1410
ORF length:
1344
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct gtggctagcc cttggcccct ttcctgccat ggagaaccag gtgctggtaa
121ttcgcatcaa gatcccaaat agtggcgcgg tggactggac agtgcactcc gggccgcagt
181tactcttcag ggatgtgctg gatgtgatag gccaggttct gcctgaagca acaactacag
241catttgaata tgaagatgaa gatggtgatc gaattacagt gagaagtgat gaggaaatga
301aggcaatgct gtcatattat tattccacag taatggaaca gcaagtaaat ggacagttaa
361tagagcctct gcagatattt ccaagagcct gcaagcctcc tggggaacgg aacatacatg
421gcctgaaggt gaatactcgg gccggaccct ctcaacacag cagcccagca gtctcagatt
481cacttccaag caatagctta aagaagtctt ctgctgaact gaaaaaaata ctagccaatg
541gccagatgaa tgaacaagac atacgatatc gggacactct tggtcatggc aacggaggca
601cagtctacaa agcatatcat gtcccgagtg ggaaaatatt agctgtaaag gtcatactac
661tagatattac actggaactt cagaagcaaa ttatgtctga attggaaatt ctttataagt
721gcgattcatc atatatcatt ggattttatg gagcattttt tgtagaaaac aggatttcaa
781tatgtacaga attcatggat gggggatctt tggatgtata taggaaaatg ccagaacatg
841tccttggaag aattgcagta gcagttgtta aaggccttac ttatttgtgg agtttaaaga
901ttttacatag agacgtgaag ccctccaata tgctagtaaa cacaagagga caggttaagc
961tgtgtgattt tggagttagc actcagctgg tgaattctat agccaagacg tatgttggaa
1021caaatgctta tatggcgcct gaaaggattt caggggagca gtatggaatt cattctgatg
1081tctggagctt aggaatctct tttatggagc ttgctcttgg gaggtttcca tatcctcaga
1141ttcagaaaaa ccagggatct ttaatgcctc tccagcttct gcagtgcatt gttgatgagg
1201attcgcccgt ccttccagtt ggagagttct cggagccatt tgtacatttc atcactcagt
1261gtatgcgaaa acagccaaaa gaaaggccag cacctgaaga attgatgggc cacccgttca
1321tcgtgcagtt caatgatgga aatgccgccg tggtgtccat gtgggtgtgc cgggcgctgg
1381aggagaggcg gagccagcag gggcccccgt gcccaacttt cttgtacaaa gtggttggta
1441agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1501ggtggcgcgt taagtcgaca atcaacctct ggatta

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