Transcript: Mouse XM_011242741.2

PREDICTED: Mus musculus mitogen-activated protein kinase kinase 5 (Map2k5), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Map2k5 (23938)
Length:
6022
CDS:
4625..5704

Additional Resources:

NCBI RefSeq record:
XM_011242741.2
NBCI Gene record:
Map2k5 (23938)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011242741.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199610 GCATTGTTGATGAGGATTCGC pLKO.1 5478 CDS 100% 2.640 2.112 N MAP2K5 n/a
2 TRCN0000320677 GCATTGTTGATGAGGATTCGC pLKO_005 5478 CDS 100% 2.640 2.112 N MAP2K5 n/a
3 TRCN0000374320 ATAGAGCCGCTGCAGATATTT pLKO_005 4652 CDS 100% 15.000 10.500 N Map2k5 n/a
4 TRCN0000025235 GCCCTCCAACATGCTTGTAAA pLKO.1 5212 CDS 100% 13.200 9.240 N Map2k5 n/a
5 TRCN0000025236 CGTGCAGTTCAATGATGGAAA pLKO.1 5614 CDS 100% 4.950 3.465 N Map2k5 n/a
6 TRCN0000322119 CGTGCAGTTCAATGATGGAAA pLKO_005 5614 CDS 100% 4.950 3.465 N Map2k5 n/a
7 TRCN0000025238 GCTGGTGATTCGGATCAAGAT pLKO.1 4473 5UTR 100% 4.950 3.465 N Map2k5 n/a
8 TRCN0000322055 GCTGGTGATTCGGATCAAGAT pLKO_005 4473 5UTR 100% 4.950 3.465 N Map2k5 n/a
9 TRCN0000025237 GCCTTACCTATCTGTGGAGTT pLKO.1 5166 CDS 100% 4.050 2.835 N Map2k5 n/a
10 TRCN0000322056 GCCTTACCTATCTGTGGAGTT pLKO_005 5166 CDS 100% 4.050 2.835 N Map2k5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011242741.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01290 pDONR223 100% 72.7% 78.1% None (many diffs) n/a
2 ccsbBroad304_01290 pLX_304 0% 72.7% 78.1% V5 (many diffs) n/a
3 ccsbBroadEn_14809 pDONR223 0% 72.7% 78.1% None (many diffs) n/a
4 ccsbBroad304_14809 pLX_304 0% 72.7% 78.1% V5 (many diffs) n/a
5 TRCN0000465680 AGGTGACCTATTCTTTCAGAAATC pLX_317 20.8% 72.7% 78.1% V5 (many diffs) n/a
6 TRCN0000488580 TGTTTACCTTTCTAAGTGAGACGG pLX_317 20.9% 72.7% 78.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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