Construct: ORF ccsbBroad304_01426
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004159.1_s304c1, BRDN0000411000
- Derived from:
- ccsbBroadEn_01426
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL23 (6150)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01426
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | NM_021134.4 | 100% | 100% | |
| 2 | human | 107987373 | LOC107987373 | 39S ribosomal protein L23, ... | XM_006724896.2 | 99.7% | 99.3% | 364A>G |
| 3 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | XM_006718271.2 | 75.3% | 64.1% | (many diffs) |
| 4 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | XM_011520276.3 | 68.2% | 53.9% | (many diffs) |
| 5 | human | 107987373 | LOC107987373 | 39S ribosomal protein L23, ... | XM_017030111.1 | 68.2% | 53.9% | (many diffs) |
| 6 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | XM_011520275.2 | 57.6% | 44.5% | (many diffs) |
| 7 | human | 6150 | MRPL23 | mitochondrial ribosomal pro... | XM_011520273.1 | 56% | 43.3% | (many diffs) |
| 8 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | NM_011288.1 | 76.9% | 84.5% | (many diffs) |
| 9 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | XM_006508517.2 | 74.4% | 81.2% | (many diffs) |
| 10 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | XM_006508516.3 | 73.8% | 81.2% | (many diffs) |
| 11 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | XM_017322050.1 | 66.5% | 69.3% | (many diffs) |
| 12 | mouse | 19935 | Mrpl23 | mitochondrial ribosomal pro... | XM_006508518.3 | 54.7% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gcggaatgtg gtgtaccccc tgtaccggct gggtggccca caacttcggg 121 tgttccgaac caacttcttc attcagctgg tgcggcccgg tgtggcccag cccgaggaca 181 ccgtgcagtt ccggatcccc atggaaatga caagggtgga cctcaggaat tacctcgagg 241 gcatctataa cgtgcccgtg gctgctgtgc ggacacgggt gcagcatggc tctaacaaga 301 gaagagatca cagaaacgtg aggatcaaga agccggacta caaggtcgcc tacgtgcagc 361 tggcccatgg acagaccttc acgttcccag atctgtttcc cgagaaagac gagagccctg 421 aaggcagcgc tgccgacgac ctctacagca tgctcgagga ggagaggcag cagaggcaga 481 gcagcgaccc gcggcggggc ggcgtcccca gctggttcgg gctgtgccca actttcttgt 541 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 601 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a