Transcript: Mouse XM_006508517.2

PREDICTED: Mus musculus mitochondrial ribosomal protein L23 (Mrpl23), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mrpl23 (19935)
Length:
1166
CDS:
653..1081

Additional Resources:

NCBI RefSeq record:
XM_006508517.2
NBCI Gene record:
Mrpl23 (19935)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006508517.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000104431 GCTCCGTGTATTTCGAACTAA pLKO.1 688 CDS 100% 5.625 7.875 N Mrpl23 n/a
2 TRCN0000352182 GCTCCGTGTATTTCGAACTAA pLKO_005 688 CDS 100% 5.625 7.875 N Mrpl23 n/a
3 TRCN0000104430 CGGAATTACCTTGAGCAGATT pLKO.1 800 CDS 100% 4.950 3.465 N Mrpl23 n/a
4 TRCN0000104434 GAGGATCAAGAAACCAGACTA pLKO.1 895 CDS 100% 4.950 3.465 N Mrpl23 n/a
5 TRCN0000352183 GAGGATCAAGAAACCAGACTA pLKO_005 895 CDS 100% 4.950 3.465 N Mrpl23 n/a
6 TRCN0000104432 GCCTGAAGATACCGTGCAGTT pLKO.1 745 CDS 100% 4.050 2.835 N Mrpl23 n/a
7 TRCN0000352257 GCCTGAAGATACCGTGCAGTT pLKO_005 745 CDS 100% 4.050 2.835 N Mrpl23 n/a
8 TRCN0000104433 TCGGAATTACCTTGAGCAGAT pLKO.1 799 CDS 100% 4.050 2.835 N Mrpl23 n/a
9 TRCN0000352258 TCGGAATTACCTTGAGCAGAT pLKO_005 799 CDS 100% 4.050 2.835 N Mrpl23 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006508517.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01426 pDONR223 100% 74.4% 81.2% None (many diffs) n/a
2 ccsbBroad304_01426 pLX_304 0% 74.4% 81.2% V5 (many diffs) n/a
3 TRCN0000466267 GAATAATCCGTTCCGCGCGCTTAT pLX_317 63.8% 74.4% 81.2% V5 (many diffs) n/a
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