Construct: ORF ccsbBroad304_01437
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014487.1_s304c1, BRDN0000405737
- Derived from:
- ccsbBroadEn_01437
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL36AL (6166)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01437
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6166 | RPL36AL | ribosomal protein L36a like | NM_001001.5 | 100% | 100% | |
| 2 | human | 6173 | RPL36A | ribosomal protein L36a | NM_021029.6 | 89.9% | 99% | (many diffs) |
| 3 | human | 100529097 | RPL36A-HNRNPH2 | RPL36A-HNRNPH2 readthrough | NM_001199973.2 | 78.8% | 83.3% | (many diffs) |
| 4 | mouse | 66483 | Rpl36al | ribosomal protein L36A-like | NM_025589.4 | 92.1% | 99% | (many diffs) |
| 5 | mouse | 19982 | Rpl36a | ribosomal protein L36A | NM_019865.5 | 88.3% | 99% | (many diffs) |
| 6 | mouse | 624713 | Gm6525 | predicted pseudogene 6525; ... | NR_036654.1 | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 384
- ORF length:
- 318
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tgggcatggt caacgtacct aaaacccgaa gaaccttctg taagaagtgt ggcaagcatc 121 agcctcacaa agtgacacag tataagaagg gcaaggattc tttgtatgcc cagggaagga 181 ggcgctatga tcggaagcag agtggctatg gtgggcagac aaagccaatt ttccggaaga 241 aggctaagac cacaaagaag attgtgctaa ggctggaatg tgttgagcct aactgcagat 301 ccaagaggat gctggccatt aagagatgca agcattttga actgggagga gataagaaga 361 gaaagggcca agtgatccag ttctacccaa ctttcttgta caaagtggtt ggtaagccta 421 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 481 gcgttaagtc gacaatcaac ctctggatta