Construct: ORF ccsbBroad304_01467
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015278.1_s304c1, BRDN0000407521
- Derived from:
- ccsbBroadEn_01467
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTKN (6242)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01467
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6242 | RTKN | rhotekin | NM_033046.2 | 100% | 100% | |
| 2 | human | 6242 | RTKN | rhotekin | XM_017004635.2 | 100% | 100% | |
| 3 | human | 6242 | RTKN | rhotekin | NM_001015055.2 | 96.1% | 92.5% | (many diffs) |
| 4 | human | 6242 | RTKN | rhotekin | NM_001015056.1 | 93.2% | 93.2% | 0_1ins111 |
| 5 | human | 6242 | RTKN | rhotekin | XM_017004636.2 | 83.6% | 80.9% | (many diffs) |
| 6 | human | 6242 | RTKN | rhotekin | XM_005264478.3 | 80.2% | 74.2% | (many diffs) |
| 7 | human | 6242 | RTKN | rhotekin | XM_017004638.2 | 78.7% | 78.7% | 0_1ins351 |
| 8 | human | 6242 | RTKN | rhotekin | XM_005264479.4 | 78.2% | 74.3% | (many diffs) |
| 9 | human | 6242 | RTKN | rhotekin | XM_017004637.2 | 77% | 74.2% | (many diffs) |
| 10 | human | 6242 | RTKN | rhotekin | XM_017004639.1 | 74.9% | 74.3% | (many diffs) |
| 11 | human | 6242 | RTKN | rhotekin | XR_939706.1 | 70.3% | (many diffs) | |
| 12 | human | 6242 | RTKN | rhotekin | XR_939705.2 | 68.8% | (many diffs) | |
| 13 | human | 6242 | RTKN | rhotekin | XR_939704.3 | 68.4% | (many diffs) | |
| 14 | human | 6242 | RTKN | rhotekin | XM_017004640.2 | 62.6% | 59.7% | (many diffs) |
| 15 | human | 6242 | RTKN | rhotekin | XR_001738881.2 | 59.9% | 1_456del;1503_1504ins169;1938_2300del | |
| 16 | mouse | 20166 | Rtkn | rhotekin | NM_009106.2 | 87.7% | 86.9% | (many diffs) |
| 17 | mouse | 20166 | Rtkn | rhotekin | NM_133641.2 | 87.7% | 86.9% | (many diffs) |
| 18 | mouse | 20166 | Rtkn | rhotekin | NM_001136227.1 | 84.6% | 82.1% | (many diffs) |
| 19 | mouse | 20166 | Rtkn | rhotekin | XM_006505806.3 | 77.8% | 73.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1716
- ORF length:
- 1650
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca ggacagattg cacatcctgg aggacctgaa tatgctctac attcggcaga 121 tggcactcag cctggaggac acggagttgc agaggaagct agaccatgag atccggatga 181 gggaaggggc ctgtaagctg ctggcagcct gctcccagcg agagcaggct ctggaggcca 241 ccaagagcct gctagtgtgc aacagccgca tcctcagcta catgggcgag ctgcagcggc 301 gcaaggaggc gcaggtgctg gggaagacaa gccggcggcc ttctgacagt ggcccgcccg 361 ctgagcgctc cccctgccgc ggccgggtct gcatctctga cctccggatt ccactcatgt 421 ggaaggacac agaatatttc aagaacaaag gtgacttgca ccgctgggct gtgttcctgc 481 tgctgcagct gggggaacac atccaggaca cagagatgat cctagtggac aggaccctca 541 cagacatctc ctttcagagc aatgtgctct tcgctgaggc ggggccagac tttgaactgc 601 ggttagagct gtatggggcc tgtgtggaag aagagggggc cctgactggc ggccccaaga 661 ggcttgccac caaactcagc agctccctgg gccgctcctc agggaggcgt gtccgggcat 721 cgctggacag tgctgggggt tcagggagca gtcccatctt gctccccacc ccagttgttg 781 gtggtcctcg ttaccacctc ttggctcaca ccacactcac cctggcagca gtgcaagatg 841 gattccgcac acatgacctc acccttgcca gtcatgagga gaaccctgcc tggctgcccc 901 tttatggtag cgtgtgttgc cgtctggcag ctcagcctct ctgcatgact cagcccactg 961 caagtggtac cctcagggtg cagcaagctg gggagatgca gaactgggca caagtgcatg 1021 gagttctgaa aggcacaaac ctcttctgtt accggcaacc tgaggatgca gacactgggg 1081 aagagccgct gcttactatt gctgtcaaca aggagactcg agtccgggca ggggagctgg 1141 accaggctct aggacggccc ttcaccctaa gcatcagtaa ccagtatggg gatgatgagg 1201 tgacacacac ccttcagaca gaaagtcggg aagcactgca gagctggatg gaggctctgt 1261 ggcagctttt ctttgacatg agccaatgga agcagtgctg tgatgaaatc atgaaaattg 1321 aaactcctgc tccccggaaa ccaccccaag cactggcaaa gcaggggtcc ttgtaccatg 1381 agatggctat tgagccgctg gatgacatcg cagcggtgac agacatcctg acccagcggg 1441 agggcgcaag gctggagaca cccccaccct ggctggcaat gtttacagac cagcctgccc 1501 tgcctaaccc ctgctcgcct gcctcagtgg ccccagcccc agactggacc caccccctgc 1561 cctgggggag accccgaacc ttttccctgg atgctgtccc cccagaccac tcccctaggg 1621 ctcgctcggt tgcccccctc ccacctcagc gatccccacg gaccagaggc ctctgcagca 1681 aaggccaacc tcgcacttgg ctccagtcac cagtgtgccc aactttcttg tacaaagtgg 1741 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1801 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta