Transcript: Human XM_017004637.2

PREDICTED: Homo sapiens rhotekin (RTKN), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RTKN (6242)
Length:
2393
CDS:
307..1629

Additional Resources:

NCBI RefSeq record:
XM_017004637.2
NBCI Gene record:
RTKN (6242)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017004637.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000428085 GCATAGGATCTGCCCAGAAGA pLKO_005 2049 3UTR 100% 4.950 3.960 N RTKN n/a
2 TRCN0000048659 CCTAAGCATCAGTAACCAGTA pLKO.1 1296 CDS 100% 4.050 3.240 N RTKN n/a
3 TRCN0000413741 TTTCTTTGACATGAGCCAATG pLKO_005 1398 CDS 100% 6.000 4.200 N RTKN n/a
4 TRCN0000048658 CCCTTTATGGTAGCGTGTGTT pLKO.1 1028 CDS 100% 4.950 3.465 N RTKN n/a
5 TRCN0000048662 GCTGCTTACTATTGCTGTCAA pLKO.1 1218 CDS 100% 4.950 3.465 N RTKN n/a
6 TRCN0000425975 GGACACAGAGATGATCCTAGT pLKO_005 636 CDS 100% 4.050 2.835 N RTKN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017004637.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01467 pDONR223 100% 77% 74.2% None (many diffs) n/a
2 ccsbBroad304_01467 pLX_304 0% 77% 74.2% V5 (many diffs) n/a
3 TRCN0000473458 TCGCGGGCTACTGGTCGATTCCGG pLX_317 31.9% 77% 74.2% V5 (many diffs) n/a
4 ccsbBroadEn_01468 pDONR223 100% 75.2% 72.5% None (many diffs) n/a
5 TRCN0000467114 ATCCATATCAAGTGAAAACGTATG pLX_317 21.7% 75.2% 72.5% V5 (many diffs) n/a
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