Construct: ORF ccsbBroad304_01528
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010483.1_s304c1, BRDN0000406319
- Derived from:
- ccsbBroadEn_01528
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SGTA (6449)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01528
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6449 | SGTA | small glutamine rich tetrat... | NM_003021.4 | 100% | 100% | |
| 2 | human | 6449 | SGTA | small glutamine rich tetrat... | XM_011528178.3 | 100% | 100% | |
| 3 | mouse | 52551 | Sgta | small glutamine-rich tetrat... | NM_001358550.1 | 82.7% | 88.5% | (many diffs) |
| 4 | mouse | 52551 | Sgta | small glutamine-rich tetrat... | XM_006513877.2 | 82.7% | 88.5% | (many diffs) |
| 5 | mouse | 52551 | Sgta | small glutamine-rich tetrat... | NM_001358549.1 | 82.5% | 88.2% | (many diffs) |
| 6 | mouse | 52551 | Sgta | small glutamine-rich tetrat... | NM_024499.2 | 82.5% | 88.2% | (many diffs) |
| 7 | mouse | 52551 | Sgta | small glutamine-rich tetrat... | XM_030245176.1 | 56.1% | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1005
- ORF length:
- 939
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga caacaagaag cgcctggcct acgccatcat ccagttcctg catgaccagc 121 tccggcacgg gggcctctcg tccgatgctc aggagagctt ggaagtcgcc atccagtgcc 181 tggagactgc gtttggggtg acggtagaag acagtgacct tgcgctccct cagactctgc 241 cggagatatt tgaagcggct gccacgggca aggagatgcc gcaggacctg aggagccccg 301 cgcgaacccc gccttccgag gaggactcag cagaggcaga gcgcctcaaa accgaaggaa 361 acgagcagat gaaagtggaa aactttgaag ctgccgtgca tttctacgga aaagccatcg 421 agctcaaccc agccaacgcc gtctatttct gcaacagagc cgcagcctac agcaaactcg 481 gcaactacgc aggcgcggtg caggactgtg agcgggccat ctgcattgac ccggcctaca 541 gcaaggccta cggcaggatg ggcctggcgc tctccagcct caacaagcac gtggaggccg 601 tggcttacta caagaaggct ctggagctgg accccgacaa cgagacatac aagtccaacc 661 tcaagatagc ggagctgaag ctgcgggagg cccccagccc cacgggaggc gtgggcagct 721 tcgacatcgc cggcctgctg aacaaccctg gcttcatgag catggcttcg aacctaatga 781 acaatcccca gattcagcag ctcatgtccg gcatgatttc gggtggcaac aaccccttgg 841 gaactcccgg caccagcccc tcgcagaacg acctggccag cctcatccag gcgggccagc 901 agtttgccca gcagatgcag cagcagaacc cagagttgat agagcagctc aggagccaga 961 tccggagtcg gacgcccagc gccagcaacg acgaccagca ggagtgccca attttcttgt 1021 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1081 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a