Transcript: Mouse XM_006513877.2

PREDICTED: Mus musculus small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha (Sgta), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Sgta (52551)
Length:
2198
CDS:
316..1260

Additional Resources:

NCBI RefSeq record:
XM_006513877.2
NBCI Gene record:
Sgta (52551)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006513877.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000190912 CCATCAGCCAATCCTAACTTT pLKO.1 1785 3UTR 100% 5.625 3.938 N Sgta n/a
2 TRCN0000293134 CCATCAGCCAATCCTAACTTT pLKO_005 1785 3UTR 100% 5.625 3.938 N Sgta n/a
3 TRCN0000189876 CCCTGACAACGACACATACAA pLKO.1 882 CDS 100% 5.625 3.938 N Sgta n/a
4 TRCN0000293131 CCCTGACAACGACACATACAA pLKO_005 882 CDS 100% 5.625 3.938 N Sgta n/a
5 TRCN0000189716 GCCACCAGAACCATCTTTGTT pLKO.1 1941 3UTR 100% 5.625 3.938 N Sgta n/a
6 TRCN0000345996 GCCACCAGAACCATCTTTGTT pLKO_005 1941 3UTR 100% 5.625 3.938 N Sgta n/a
7 TRCN0000189473 CCTCCTAAAGGGACTTGAGTA pLKO.1 1572 3UTR 100% 4.950 3.465 N Sgta n/a
8 TRCN0000190560 GCTGTCTACTTCTGTAACAGA pLKO.1 688 CDS 100% 3.000 2.100 N Sgta n/a
9 TRCN0000293068 GCTGTCTACTTCTGTAACAGA pLKO_005 688 CDS 100% 3.000 2.100 N Sgta n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006513877.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01528 pDONR223 100% 82.7% 88.5% None (many diffs) n/a
2 ccsbBroad304_01528 pLX_304 0% 82.7% 88.5% V5 (many diffs) n/a
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