Construct: ORF ccsbBroad304_01585

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016724.1_s304c1, BRDN0000462012
Derived from:
ccsbBroadEn_01585
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SPAST (6683)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01585
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6683 SPAST spastin NM_199436.1 100% 100%
2 human 6683 SPAST spastin NM_001363875.1 99.8% 99.8% 413_414insAGC
3 human 6683 SPAST spastin NM_014946.3 94.8% 94.8% 585_680del
4 human 6683 SPAST spastin NM_001363823.1 94.6% 94.6% 413_414insAGC;582_677del
5 human 6683 SPAST spastin XM_017004778.2 88.8% 87.1% 1518_1519ins71;1557_1558ins124
6 human 6683 SPAST spastin XM_011533067.2 84.2% 82.6% 585_680del;1614_1615ins71;1653_1654ins124
7 mouse 50850 Spast spastin XM_006524610.3 88.3% 92.8% (many diffs)
8 mouse 50850 Spast spastin XM_006524611.3 88.1% 92.6% (many diffs)
9 mouse 50850 Spast spastin NM_001162870.1 83.7% 87.9% (many diffs)
10 mouse 50850 Spast spastin NM_016962.2 83.5% 87.8% (many diffs)
11 mouse 50850 Spast spastin XM_011246530.2 52.3% 57.3% (many diffs)
12 mouse 50850 Spast spastin XR_001782088.1 24.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1821
ORF length:
1752
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaattctccg ggtggacgag ggaagaagaa aggctccggc ggcgccagca
121acccggtgcc tcccaggcct ccgccccctt gcctggcccc cgcccctccc gccgccgggc
181cggcccctcc gcccgagtcg ccgcataagc ggaacctgta ctatttctcc tacccgctgt
241ttgtaggctt cgcgctgctg cgtttggtcg ccttccacct ggggctcctc ttcgtgtggc
301tctgccagcg cttctcccgc gccctcatgg cagccaagag gagctccggg gccgcgccag
361cacctgcctc ggcctcggcc ccggcgccgg tgccgggcgg cgaggccgag cgcgtccgag
421tcttccacaa acaggccttc gagtacatct ccattgccct gcgcatcgat gaggatgaga
481aagcaggaca gaaggagcaa gctgtggaat ggtataagaa aggtattgaa gaactggaaa
541aaggaatagc tgttatagtt acaggacaag gtgaacagtg tgaaagagct agacgccttc
601aagctaaaat gatgactaat ttggttatgg ccaaggaccg cttacaactt ctagaaagtg
661gagctgttcc aaaaagaaaa gaccccttaa cacacactag taattcactg cctcgttcaa
721aaacagttat gaaaactgga tctgcaggcc tttcaggcca ccatagagca cctagttaca
781gtggtttatc catggtttct ggagtgaaac agggatctgg tcctgctcct accactcata
841agggtactcc gaaaacaaat aggacaaata aaccttctac ccctacaact gctactcgta
901agaaaaaaga cttgaagaat tttaggaatg tggacagcaa ccttgctaac cttataatga
961atgaaattgt ggacaatgga acagctgtta aatttgatga tatagctggt caagacttgg
1021caaaacaagc attgcaagaa attgttattc ttccttctct gaggcctgag ttgttcacag
1081ggcttagagc tcctgccaga gggctgttac tctttggtcc acctgggaat gggaagacaa
1141tgctggctaa agcagtagct gcagaatcga atgcaacctt ctttaatata agtgctgcaa
1201gtttaacttc aaaatacgtg ggagaaggag agaaattggt gagggctctt tttgctgtgg
1261ctcgagaact tcaaccttct ataattttta tagatgaagt tgatagcctt ttgtgtgaaa
1321gaagagaagg ggagcacgat gctagtagac gcctaaaaac tgaatttcta atagaatttg
1381atggtgtaca gtctgctgga gatgacagag tacttgtaat gggtgcaact aataggccac
1441aagagcttga tgaggctgtt ctcaggcgtt tcatcaaacg ggtatatgtg tctttaccaa
1501atgaggagac aagactactt ttgcttaaaa atctgttatg taaacaagga agtccattga
1561cccaaaaaga actagcacaa cttgctagaa tgactgatgg atactcagga agtgacctaa
1621cagctttggc aaaagatgca gcactgggtc ctatccgaga actaaaacca gaacaggtga
1681agaatatgtc tgccagtgag atgagaaata ttcgattatc tgacttcact gaatccttga
1741aaaaaataaa acgcagcgtc agccctcaaa ctttagaagc gtacatacgt tggaacaagg
1801actttggaga taccactgtt ttgccaactt tcttgtacaa agtggttggt aagcctatcc
1861ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1921ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)