Transcript: Mouse NM_001162870.1

Mus musculus spastin (Spast), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Spast (50850)
Length:
4696
CDS:
51..1895

Additional Resources:

NCBI RefSeq record:
NM_001162870.1
NBCI Gene record:
Spast (50850)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001162870.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000311200 GCCCATAGAACATCGCACTTC pLKO_005 1916 3UTR 100% 4.050 5.670 N Spast n/a
2 TRCN0000101592 GCTGTTATAGTTACGGGCCAA pLKO.1 525 CDS 100% 2.160 3.024 N Spast n/a
3 TRCN0000304960 CAATCTTGCTAACCTTATAAT pLKO_005 1010 CDS 100% 15.000 12.000 N Spast n/a
4 TRCN0000421045 GATGAGAAATATTCGATTATC pLKO_005 1772 CDS 100% 13.200 10.560 N SPAST n/a
5 TRCN0000101591 CGTTTCATTAAACGGGTATAT pLKO.1 1539 CDS 100% 1.320 1.056 N Spast n/a
6 TRCN0000308338 CGTTTCATTAAACGGGTATAT pLKO_005 1539 CDS 100% 1.320 1.056 N Spast n/a
7 TRCN0000101594 CCCTTAACACATGCTAGTAAT pLKO.1 756 CDS 100% 13.200 9.240 N Spast n/a
8 TRCN0000308404 CCCTTAACACATGCTAGTAAT pLKO_005 756 CDS 100% 13.200 9.240 N Spast n/a
9 TRCN0000101593 CGAGAACTTCAACCATCTATA pLKO.1 1335 CDS 100% 13.200 7.920 N Spast n/a
10 TRCN0000308336 CGAGAACTTCAACCATCTATA pLKO_005 1335 CDS 100% 13.200 7.920 N Spast n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001162870.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01585 pDONR223 100% 83.7% 87.9% None (many diffs) n/a
2 ccsbBroad304_01585 pLX_304 0% 83.7% 87.9% V5 (many diffs) n/a
3 TRCN0000478063 CTGGGAGATCTCTGGGCAATCGTG pLX_317 25.4% 83.7% 87.9% V5 (many diffs) n/a
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