Construct: ORF ccsbBroad304_01594

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000503.1_s304c1, BRDN0000409656
Derived from:
ccsbBroadEn_01594
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRPK2 (6733)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01594
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6733 SRPK2 SRSF protein kinase 2 NM_001278273.1 100% 100%
2 human 6733 SRPK2 SRSF protein kinase 2 NM_001350738.1 100% 100%
3 human 6733 SRPK2 SRSF protein kinase 2 NM_182691.2 100% 100%
4 human 6733 SRPK2 SRSF protein kinase 2 NM_001350739.1 98% 98.5% (many diffs)
5 human 6733 SRPK2 SRSF protein kinase 2 NM_182692.3 97.5% 96.7% (many diffs)
6 human 6733 SRPK2 SRSF protein kinase 2 NM_001350743.1 95.6% 95.6% 1881_1973del
7 human 6733 SRPK2 SRSF protein kinase 2 NM_001350744.1 95.6% 95.6% 1881_1973del
8 human 6733 SRPK2 SRSF protein kinase 2 XM_006716098.3 95.6% 95.6% 1881_1973del
9 human 6733 SRPK2 SRSF protein kinase 2 XM_011516538.3 95.6% 95.6% 1881_1973del
10 human 6733 SRPK2 SRSF protein kinase 2 NM_001350742.1 94.8% 94.8% 1_111del
11 human 6733 SRPK2 SRSF protein kinase 2 NM_001350741.1 93.3% 92.6% (many diffs)
12 human 6733 SRPK2 SRSF protein kinase 2 NM_001350745.1 93.3% 93.1% 616_617ins138
13 human 6733 SRPK2 SRSF protein kinase 2 XM_011516536.3 93% 92.7% (many diffs)
14 human 6733 SRPK2 SRSF protein kinase 2 XM_017012561.2 93% 93.5% (many diffs)
15 human 6733 SRPK2 SRSF protein kinase 2 NM_001350740.1 92.5% 91.8% (many diffs)
16 human 6733 SRPK2 SRSF protein kinase 2 XM_024446893.1 91% 91% 1_111del;1992_2084del
17 human 6733 SRPK2 SRSF protein kinase 2 XM_024446895.1 90.6% 90.4% (many diffs)
18 human 6733 SRPK2 SRSF protein kinase 2 XM_024446894.1 88.7% 88.1% (many diffs)
19 human 6733 SRPK2 SRSF protein kinase 2 NM_001350746.1 80.1% 78.2% 1_111del;1829_1830ins163;1854_1855ins158
20 human 6733 SRPK2 SRSF protein kinase 2 XR_001744860.2 60.5% 1_180del;2061_2153del;2338_3409del
21 human 6733 SRPK2 SRSF protein kinase 2 XR_001744863.2 49.7% 1_173del;2238_4151del
22 human 6733 SRPK2 SRSF protein kinase 2 XR_001744861.2 48.5% 1_180del;2061_2153del;2338_4251del
23 human 6733 SRPK2 SRSF protein kinase 2 XR_001744862.2 45.6% 1_180del;2061_2153del;2338_4518del
24 human 6733 SRPK2 SRSF protein kinase 2 XR_001744859.2 43.7% 1_180del;2061_2153del;2338_4714del
25 mouse 20817 Srpk2 serine/arginine-rich protei... NM_009274.2 89.8% 94.1% (many diffs)
26 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320769.1 89.8% 94.1% (many diffs)
27 mouse 20817 Srpk2 serine/arginine-rich protei... XM_006535661.3 87.3% 91% (many diffs)
28 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249755.2 86% 90.1% (many diffs)
29 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249756.2 86% 90.1% (many diffs)
30 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249757.2 86% 90.1% (many diffs)
31 mouse 20817 Srpk2 serine/arginine-rich protei... XM_006535660.3 85.3% 89.3% (many diffs)
32 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320767.1 84.1% 88.1% (many diffs)
33 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249753.2 83.6% 87.1% (many diffs)
34 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249754.2 83.6% 88% (many diffs)
35 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320768.1 82.4% 86.7% (many diffs)
36 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249751.2 81.8% 85.7% (many diffs)
37 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249752.2 80.7% 84.5% (many diffs)
38 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320771.1 76.5% 80.1% (many diffs)
39 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249758.2 73.2% 76.6% (many diffs)
40 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320770.1 73.2% 76.6% (many diffs)
41 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320774.1 69.3% 70.3% (many diffs)
42 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320776.1 68.1% 69% (many diffs)
43 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320775.1 68% 70.9% (many diffs)
44 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320777.1 66.8% 69.6% (many diffs)
45 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320773.1 66.3% 67.3% (many diffs)
46 mouse 20817 Srpk2 serine/arginine-rich protei... XM_017320772.1 65.2% 66.1% (many diffs)
47 mouse 20817 Srpk2 serine/arginine-rich protei... XM_011249759.2 65.1% 67.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2130
ORF length:
2064
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc agttaactct gagaagtcgt cctcttcaga aaggccggag cctcaacaga
121aagctccttt agttcctcct cctccaccgc caccaccacc accaccgcca cctttgccag
181accccacacc cccggagcca gaggaggaga tcctgggatc agatgatgag gagcaagagg
241accctgcgga ctactgcaaa ggtggatatc atccagtgaa aattggagac ctcttcaatg
301gccggtatca tgttattaga aagcttggat gggggcactt ctctactgtc tggctgtgct
361gggatatgca ggggaaaaga tttgttgcaa tgaaagttgt aaaaagtgcc cagcattata
421cggagacagc cttggatgaa ataaaattgc tcaaatgtgt tcgagaaagt gatcccagtg
481acccaaacaa agacatggtg gtccagctca ttgacgactt caagatttca ggcatgaatg
541ggatacatgt ctgcatggtc ttcgaagtac ttggccacca tctcctcaag tggatcatca
601aatccaacta tcaaggcctc ccagtacgtt gtgtgaagag tatcattcga caggtccttc
661aagggttaga ttacttacac agtaagtgca agatcattca tactgacata aagccggaaa
721atatcttgat gtgtgtggat gatgcatatg tgagaagaat ggcagctgag gccactgagt
781ggcagaaagc aggtgctcct cctccttcag ggtctgcagt gagtacggct ccacagcaga
841aacctatagg aaaaatatct aaaaacaaaa agaaaaaact gaaaaagaaa cagaagaggc
901aggctgagtt attggagaag cgcctgcagg agatagaaga attggagcga gaagctgaaa
961ggaaaataat agaagaaaac atcacctcag ctgcaccttc caatgaccag gatggcgaat
1021actgcccaga ggtgaaacta aaaacaacag gattagagga ggcggctgag gcagagactg
1081caaaggacaa tggtgaagct gaggaccagg aagagaaaga agatgctgag aaagaaaaca
1141ttgaaaaaga tgaagatgat gtagatcagg aacttgcgaa catagaccct acgtggatag
1201aatcacctaa aaccaatggc catattgaga atggcccatt ctcactggag cagcaactgg
1261acgatgaaga tgatgatgaa gaagactgcc caaatcctga ggaatataat cttgatgagc
1321caaatgcaga aagtgattac acatatagca gctcctatga acaattcaat ggtgaattgc
1381caaatggacg acataaaatt cccgagtcac agttcccaga gttttccacc tcgttgttct
1441ctggatcctt agaacctgtg gcctgcggct ctgtgctttc tgagggatca ccacttactg
1501agcaagagga gagcagtcca tcccatgaca gaagcagaac ggtttcagcc tccagtactg
1561gggatttgcc aaaagcaaaa acccgggcag ctgacttgtt ggtgaatccc ctggatccgc
1621ggaatgcaga taaaattaga gtaaaaattg ctgacctggg aaatgcttgt tgggtgcata
1681aacacttcac ggaagacatc cagacgcgtc agtaccgctc catagaggtt ttaataggag
1741cggggtacag cacccctgcg gacatctgga gcacggcgtg tatggcattt gagctggcaa
1801cgggagatta tttgtttgaa ccacattctg gggaagacta ttccagagac gaagaccaca
1861tagcccacat catagagctg ctaggcagta ttccaaggca ctttgctcta tctggaaaat
1921attctcggga attcttcaat cgcagaggag aactgcgaca catcaccaag ctgaagccct
1981ggagcctctt tgatgtactt gtggaaaagt atggctggcc ccatgaagat gctgcacagt
2041ttacagattt cctgatcccg atgttagaaa tggttccaga aaaacgagcc tcagctggcg
2101aatgccttcg gcatccttgg ttgaattctt acccaacttt cttgtacaaa gtggttggta
2161agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
2221ggtggcgcgt taagtcgaca atcaacctct ggatta

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