Transcript: Mouse XM_006535661.3

PREDICTED: Mus musculus serine/arginine-rich protein specific kinase 2 (Srpk2), transcript variant X11, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Srpk2 (20817)
Length:
5202
CDS:
131..2212

Additional Resources:

NCBI RefSeq record:
XM_006535661.3
NBCI Gene record:
Srpk2 (20817)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006535661.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361471 GCTCGCCACAGGAGACTATTT pLKO_005 1873 CDS 100% 13.200 18.480 N Srpk2 n/a
2 TRCN0000023166 CCAAACAAAGACATGGTAGTT pLKO.1 575 CDS 100% 4.950 6.930 N Srpk2 n/a
3 TRCN0000023164 GCAGAGAGTGATTACACGTAT pLKO.1 1421 CDS 100% 4.950 6.930 N Srpk2 n/a
4 TRCN0000023165 GATCTCTTCAATGGTCGATAT pLKO.1 380 CDS 100% 10.800 8.640 N Srpk2 n/a
5 TRCN0000006277 CGTTGTGTGAAGAGTATCATT pLKO.1 719 CDS 100% 5.625 4.500 N SRPK2 n/a
6 TRCN0000277860 CGTTGTGTGAAGAGTATCATT pLKO_005 719 CDS 100% 5.625 4.500 N SRPK2 n/a
7 TRCN0000361474 ACATGGTAGTTCAGCTAATTG pLKO_005 585 CDS 100% 13.200 9.240 N Srpk2 n/a
8 TRCN0000361472 GCAAATCCCGAGGAGTATAAC pLKO_005 1385 CDS 100% 13.200 9.240 N Srpk2 n/a
9 TRCN0000361544 TGTCTTCAGCTAAGTAGTTTA pLKO_005 2402 3UTR 100% 13.200 9.240 N Srpk2 n/a
10 TRCN0000361473 TAGCATTCTGAGCTAGCAAAT pLKO_005 2228 3UTR 100% 10.800 7.560 N Srpk2 n/a
11 TRCN0000197040 GAGACAGCCTTGGATGAAATA pLKO.1 515 CDS 100% 13.200 9.240 N SRPK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006535661.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01594 pDONR223 100% 87.3% 91% None (many diffs) n/a
2 ccsbBroad304_01594 pLX_304 0% 87.3% 91% V5 (many diffs) n/a
3 ccsbBroadEn_14847 pDONR223 0% 87.3% 91% None (many diffs) n/a
4 ccsbBroad304_14847 pLX_304 0% 87.3% 91% V5 (many diffs) n/a
5 TRCN0000466082 GGAGGCGATTGTTTTCGAACATGA pLX_317 15.5% 87.3% 90.8% V5 (many diffs) n/a
6 TRCN0000489739 AGAACCGGCTTCTTCCAGTACGTG pLX_317 21.4% 87.3% 91% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000489410 TGGGACTGCATACCAAAGATACGG pLX_317 16.7% 87.3% 90.8% V5 (many diffs) n/a
8 ccsbBroadEn_06999 pDONR223 100% 87.2% 90.7% None (many diffs) n/a
9 ccsbBroad304_06999 pLX_304 0% 87.2% 90.7% V5 (many diffs) n/a
10 TRCN0000473240 CAAGGCTGTACCTCATATTCCTAA pLX_317 19% 87.2% 90.7% V5 (many diffs) n/a
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